Supercontinuum source-based multi-contrast optical coherence tomography for rat retina imaging

Syu, Jia Pu; Buddhakosai, Waradee; Chen, Shih‐Jen; Ke, Chang Chih; Chiou, Shih Hwa; Kuo, Wen Chuan

doi:10.1364/boe.9.006132

Cited by 14 publications

(10 citation statements)

References 43 publications

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“…After anesthesia with a mixed solution of Zoletil (50 mg/kg) and Ropum (10 mg/kg), a mouse retinal structure image was obtained with multi-contrast optical coherence tomography (OCT), according to previous reports [ 23 ]. The fundus photography images were acquired with a retinal imaging camera (Nikon, Shinagawa, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Inhibition of DUSP6 Activates Autophagy and Rescues the Retinal Pigment Epithelium in Sodium Iodate-Induced Retinal Degeneration Models In Vivo and In Vitro

et al. 2022

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Autophagy plays a protective role in the retinal pigment epithelium (RPE) by eliminating damaged organelles in response to reactive oxygen species (ROS). Dual-specificity protein phosphatase 6 (DUSP6), which belongs to the DUSP subfamily, works as a negative-feedback regulator of the extracellular signal-regulated kinase (ERK) pathway. However, the complex interplay between DUSP6 and autophagy induced by ROS in RPE is yet to be investigated. To investigate the relationship between DUSP6 and autophagy, we exposed the ARPE-19 cell line and C57BL/6N mice to sodium iodate (NaIO3) as an oxidative stress inducer. Our data showed that the inhibition of DUSP6 activity promotes autophagy flux through the ERK pathway via the upregulation of immunoblotting expression in ARPE-19 cells. Live imaging showed a significant increase in autophagic flux activities, which suggested the restoration autophagy after treatment with the DUSP6 inhibitor. Furthermore, the mouse RPE layer exhibited an irregular structure and abnormal deposits following NaIO3 injection. The retina layer was recovered after being treated with DUSP6 inhibitor; this suggests that DUSP6 inhibitor can rescue retinal damage by restoring the mouse retina’s autophagy flux. This study suggests that the upregulation of DUSP6 can cause autophagy flux malfunctions in the RPE. The DUSP6 inhibitor can restore autophagy induction, which may serve as a potential therapeutic approach for retinal degeneration disease.

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Section: Methodsmentioning

confidence: 99%

Inhibition of DUSP6 Activates Autophagy and Rescues the Retinal Pigment Epithelium in Sodium Iodate-Induced Retinal Degeneration Models In Vivo and In Vitro

et al. 2022

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“…A fiber polarization beam-splitter (FPBS) with a light polarization controller (PC), PC1, was used to get pure, linearly polarized light and maximize its output power. The SS PS-OCT system was constructed with all single mole fibers, as proposed by our lab in 2014 and 2018 [ 38 , 39 ]. A 50/50 coupler was arranged to split the light power into 50% incident power for the reference mirror (with an attenuator), with the remaining 50% incident power (~7 mW) focused on a scanning spot.…”

Section: Methodsmentioning

confidence: 99%

Detection of Oral Dysplastic and Early Cancerous Lesions by Polarization-Sensitive Optical Coherence Tomography

Chen

Lee²,

Chen³

et al. 2020

Cancers

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Detection of oral dysplastic and early-stage cancerous lesions is difficult with the current tools. Half of oral cancers are diagnosed in a late stage. Detection of early stromal change to predict malignant transformation is a new direction in the diagnosis of early-stage oral cancer. The application of new optical tools to image stroma in vivo is under investigation, and polarization-sensitive optical coherence tomography (PS-OCT) is potentially one of those tools. This is a preliminary study to sequentially image oral stromal changes from normal, hyperplasia, and dysplasia to early-stage cancer by PS-OCT in vivo. We used 4-Nitroquinoline-1-oxide drinking water to induce dysplasia and early-stage oral cancer in 19 K14-EGFP-miR-211-GFP transgenic mice. A total of 8 normal, 12 hyperplastic, 11 dysplastic, and 4 early-stage cancerous lesions were enrolled. A new analytic process of PS-OCT imaging was proposed, called an en-face birefringence map. From the birefringence map, the sensitivity, specificity, positive predictive value, and negative predictive values to detect dysplasia and early-stage cancer were 100.00%, 95.00%, 93.75%, and 100.00%, respectively, and the kappa value of these images between two investigators was 0.942. The mean size of malignant lesions detected in this study is 1.66 ± 0.93 mm. This pilot animal study validates the use of PS-OCT to detect small and early-stage oral malignancy with high accuracy and consistency.

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“…Supercontinuum (SC), which covers broad spectral band, offers an attractive optical source in diverse applications like metrology [1,2], biomedical imaging [3][4][5], and astronomy [6]. The SC in the ultraviolet-blue region is highly useful for fluorescence microscopy because many fluorescent molecules are excited in the wavelength range from ~600 to ~350 nm [7,8].…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet-Extended Supercontinuum Generation in Zero-Dispersion Wavelength Decreasing Photonic Crystal Fibers

Liao

et al. 2020

IEEE Photonics J.

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Supercontinuum covering the ultraviolet-blue region is highly useful for fluorescence microscopy. Four zero-dispersion wavelength decreasing photonic crystal fibers with different fiber cross structures and taper profiles are fabricated to extend the short wavelength edge of supercontinuum. Both nanosecond and picosecond pump pulses at 1 μm are used to generate supercontinuum. With a 3 ns pump pulse, the short wavelength edge of supercontinuum is extended to below 400 nm in a fiber with high air-hole ratio (named T3). The underlying mechanism of supercontinuum generation is explored. The short and long wavelength edges of supercontinuum are highly related with the phase-matching condition which decided by the group velocity curve of fiber small core end. With a 10 ps pump pulse, the spectral intensity around ~800 nm increases in all four fibers. However, the intensity in shorter wavelength band decreased in fibers with a high air-hole ratio (named T3, T4). The experimental results imply that a zero-dispersion wavelength decreasing photonic crystal fiber suitable for nanosecond pulse pumping is not necessarily suitable for picosecond pulse pumping, especially for fibers with high air-hole ratio.

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Supercontinuum source-based multi-contrast optical coherence tomography for rat retina imaging

Cited by 14 publications

References 43 publications

Inhibition of DUSP6 Activates Autophagy and Rescues the Retinal Pigment Epithelium in Sodium Iodate-Induced Retinal Degeneration Models In Vivo and In Vitro

Inhibition of DUSP6 Activates Autophagy and Rescues the Retinal Pigment Epithelium in Sodium Iodate-Induced Retinal Degeneration Models In Vivo and In Vitro

Detection of Oral Dysplastic and Early Cancerous Lesions by Polarization-Sensitive Optical Coherence Tomography

Ultraviolet-Extended Supercontinuum Generation in Zero-Dispersion Wavelength Decreasing Photonic Crystal Fibers

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