Super resolution microscopy reveals altered desmosome organization, endocytosis and desmosome splitting in pemphigus vulgaris epidermis

Stahley, Sara N.; Warren, Maxine; Feldman, Ron J.; Mattheyses, Alexa L.; Kowalczyk, Andrew P.

doi:10.1016/j.jdermsci.2016.08.086

Cited by 2 publications

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“…The high resolution of dSTORM is ideal to identify and quantify the protein organization of desmosomes. SIM and dSTROM elucidate the plaque mirror symmetry, desmosomal plaque length and plaque-to-plaque distance in epithelial cells ( Figure 3C2 ) ( Stahley S. N. et al, 2016 ). Results show that desmoplakin is further localized from the plasma membrane than then previously observed with immunogold studies; i.e., desmoplakin is oriented with its long axis at an angle in the plaque and not perpendicular to the plasma membrane.…”

Section: Light-based Microscopy Techniquesmentioning

confidence: 99%

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Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale

Vanslembrouck

Chen

Larabell

et al. 2022

Front. Cell Dev. Biol.

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Considerable progress has been made in our knowledge of the morphological and functional varieties of anchoring junctions. Cell-cell adhesion contacts consist of discrete junctional structures responsible for the mechanical coupling of cytoskeletons and allow the transmission of mechanical signals across the cell collective. The three main adhesion complexes are adherens junctions, tight junctions, and desmosomes. Microscopy has played a fundamental role in understanding these adhesion complexes on different levels in both physiological and pathological conditions. In this review, we discuss the main light and electron microscopy techniques used to unravel the structure and composition of the three cell-cell contacts in epithelial and endothelial cells. It functions as a guide to pick the appropriate imaging technique(s) for the adhesion complexes of interest. We also point out the latest techniques that have emerged. At the end, we discuss the problems investigators encounter during their cell-cell adhesion research using microscopic techniques.

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Section: Light-based Microscopy Techniquesmentioning

confidence: 99%

“…Region also imaged with WF (wide field) microscopy, SIM and dSTORM. Bottom part: dSTORM image of keratinocyte cell-cell border labeled for the desmoglein-3 (Dsg3) N terminal (green) and desmoplakin C-terminal (red) domains ( Stahley S. N. et al, 2016 ). (C2) : Localization of E-cadherin with actin visible by SIM.…”

Section: Introductionmentioning

confidence: 99%

Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale

Vanslembrouck

Chen

Larabell

et al. 2022

Front. Cell Dev. Biol.

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“…In PV patient tissue desmosome size was reduced and Dsg3 was found to be aberrantly clustered and localized in "linear arrays". 103 The mirror symmetry created by desmosomal plaques was visible in the SIM images, which allowed identification of desmosomes "split" along the adhesive interface at blister sites. Desmosome splitting was recapitulated in vitro by exposing cultured keratinocytes to PV IgG and to mechanical stress, demonstrating that mechanical stress can lead to desmosome splitting.…”

Section: Desmosomesmentioning

confidence: 98%

Bridging the gap: Super-resolution microscopy of epithelial cell junctions

et al. 2018

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Cell junctions are critical for cell adhesion and communication in epithelial tissues. It is evident that the cellular distribution, size, and architecture of cell junctions play a vital role in regulating function. These details of junction architecture have been challenging to elucidate in part due to the complexity and size of cell junctions. A major challenge in understanding these features is attaining high resolution spatial information with molecular specificity. Fluorescence microscopy allows localization of specific proteins to junctions, but with a resolution on the same scale as junction size, rendering internal protein organization unobtainable. Super-resolution microscopy provides a bridge between fluorescence microscopy and nanoscale approaches, utilizing fluorescent tags to reveal protein organization below the resolution limit. Here we provide a brief introduction to super-resolution microscopy and discuss novel findings into the organization, structure and function of epithelial cell junctions.

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Super resolution microscopy reveals altered desmosome organization, endocytosis and desmosome splitting in pemphigus vulgaris epidermis

Cited by 2 publications

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Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale

Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale

Bridging the gap: Super-resolution microscopy of epithelial cell junctions

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