Super‐Resolution Microscopy: Going Live and Going Fast

Lakadamyali, Melike

doi:10.1002/cphc.201300720

Cited by 39 publications

(32 citation statements)

References 66 publications

(153 reference statements)

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“…Although we have implemented this protocol on fixed brain samples, a number of alternative methods are viable on live samples 23 . Importantly, the STORM method itself has been demonstrated to be suitable for multichannel detection 24,25 , and for live-cell imaging 26 of labeled proteins 27 , membrane dyes 28 or fluorescent proteins 29 . Detailed protocols for imaging of cell cultures have been published for both dSTORM and PALM imaging methods [30][31][32] .…”

Section: Applications Of the Methodsmentioning

confidence: 99%

Correlated confocal and super-resolution imaging by VividSTORM

et al. 2015

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Single-molecule localization microscopy (SMLM) is rapidly gaining popularity in the life sciences as an efficient approach to visualize molecular distribution with nanoscale precision. However, it has been challenging to obtain and analyze such data within a cellular context in tissue preparations. Here we describe a 5-d tissue processing and immunostaining procedure that is optimized for SMLM, and we provide example applications to fixed mouse brain, heart and kidney tissues. We then describe how to perform correlated confocal and 3D-superresolution imaging on these sections, which allows the visualization of nanoscale protein localization within labeled subcellular compartments of identified target cells in a few minutes. Finally, we describe the use of VividSTORM (http://katonalab.hu/index.php/vividstorm), an open-source software for correlated confocal and SMLM image analysis, which facilitates the measurement of molecular abundance, clustering, internalization, surface density and intermolecular distances in a cell-specific and subcellular compartment-restricted manner. The protocol requires only basic skills in tissue staining and microscopy.

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Section: Applications Of the Methodsmentioning

confidence: 99%

Correlated confocal and super-resolution imaging by VividSTORM

et al. 2015

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“…At this point in time, nanoscopy experiments are still quite challenging to perform and, despite the recent availability of commercial equipment, it will take time before they will turn into standard approaches [12,13].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Where Do We Stand with Super-Resolution Optical Microscopy?

Nienhaus

2016

Journal of Molecular Biology

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“…3D STORM also plays an important role in analyzing the organization of chromatin in transcriptional active state. 47 When it comes to living cells, it is shown that the spatial resolution could achieve 20-40 nm while keeping the temporal resolution at 0.5-10 s. 48 As (F)PALM/STORM greatly depends on°uorophores, by combing biomolecular°uores-cence complementation and PALM, Liu et al successfully imaged the location and dynamics of MreB and Ef-Tu in Escherichia coli cell. 49 Li et al reported a trident perylenemonoimide dye modi¯ed by triple dithienylethenes which has high sensitivity of on/o® switching and with its help, images of vesicles within sub-100 nm resolution can be achieved.…”

Section: Photoactivation Localization Microscopy (Palm)/stochastic Opmentioning

confidence: 99%

Super-resolution microscopy and its applications in neuroscience

Wang

Zhu

et al. 2017

J. Innov. Opt. Health Sci.

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Optical microscopy promises researchers to see most tiny substances directly. However, the resolution of conventional microscopy is restricted by the di®raction limit. This makes it a challenge to observe subcellular processes happened in nanoscale. The development of superresolution microscopy provides a solution to this challenge. Here, we brie°y review several commonly used super-resolution techniques, explicating their basic principles and applications in biological science, especially in neuroscience. In addition, characteristics and limitations of each technique are compared to provide a guidance for biologists to choose the most suitable tool.

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Super‐Resolution Microscopy: Going Live and Going Fast

Cited by 39 publications

References 66 publications

Correlated confocal and super-resolution imaging by VividSTORM

Correlated confocal and super-resolution imaging by VividSTORM

Where Do We Stand with Super-Resolution Optical Microscopy?

Super-resolution microscopy and its applications in neuroscience

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