Super-resolution fluorescence imaging of biocompatible carbon dots

Leménager, Godefroy; Luca, Elisa De; Sun, Ya‐Ping; Pompa, Pier Paolo

doi:10.1039/c4nr01970a

Cited by 102 publications

(81 citation statements)

References 22 publications

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“…The unique PL characteristics of C-dots have already attracted lots of interest for the labeling and imaging applications [40][41][42][43] . Here in this paper, we have demonstrated two typical and potential applications for labeling nanostructures and cells and their imaging under microscopy.…”

Section: Labeling and Imagingmentioning

confidence: 99%

One-step, green, and economic synthesis of water-soluble photoluminescent carbon dots by hydrothermal treatment of wheat straw, and their bio-applications in labeling, imaging, and sensing

Yuan

Zhong

Gao

et al. 2015

Applied Surface Science

144

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Section: Labeling and Imagingmentioning

confidence: 99%

One-step, green, and economic synthesis of water-soluble photoluminescent carbon dots by hydrothermal treatment of wheat straw, and their bio-applications in labeling, imaging, and sensing

Yuan

Zhong

Gao

et al. 2015

Applied Surface Science

144

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“…Among more significant recent successes have been the finding and subsequent development of carbon "quantum" dots or more appropriately called carbon dots (for the lack of the classical quantum confinement effect in these nanomaterials), [4][5][6][7][8][9][10][11] which have played a leading role in an emerging and rapidly -3 -expanding research field centered on the design, preparation, and potential biomedical uses of various carbon-based QDs. [12][13][14][15][16][17][18][19][20][21][22][23][24][25] Carbon dots are generally small carbon nanoparticles with various surface passivation schemes by organic or bio-molecules (Figure 1), 4,6,7,12 where the more effective surface passivation has been correlated with brighter fluorescence emissions from the corresponding dots. The optical absorption of carbon dots is assigned to π-plasmon transitions in the carbon nanoparticle core of the dots, while the fluorescence emissions in the visible to near-IR are attributed to photogenerated electrons and holes trapped at diverse surface sites and their associated radiative recombinations.…”

Section: Introductionmentioning

confidence: 99%

Carbon “Quantum” Dots for Fluorescence Labeling of Cells

Liu

Cao

LeCroy

et al. 2015

ACS Appl. Mater. Interfaces

Self Cite

150

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The specifically synthesized and selected carbon dots of relatively high fluorescence quantum yields were evaluated in their fluorescence labeling of cells. For the cancer cell lines, the cellular uptake of the carbon dots was generally efficient, resulting in the labeling of the cells with bright fluorescence emissions for both one- and two-photon excitations from predominantly the cell membrane and cytoplasm. In the exploration on labeling the live stem cells, the cellular uptake of the carbon dots was relatively less efficient, though fluorescence emissions could still be adequately detected in the labeled cells, with the emissions again predominantly from the cell membrane and cytoplasm. This combined with the observed more efficient internalization of the same carbon dots by the fixed stem cells might suggest some significant selectivity of the stem cells toward surface functionalities of the carbon dots. The needs and possible strategies for more systematic and comparative studies on the fluorescence labeling of different cells, including especially live stem cells, by carbon dots as a new class of brightly fluorescent probes are discussed.

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“…In biological experiments, the GQDs were found to be suitable for the imaging of T47D cells and showed a low cytotoxicity. 232 Toward this, CDs with diameters of approx. 223 In a first step, polymer-like CDs were prepared from citric acid and ethylenediamine, which were then converted in a hydrothermal reaction to CDs.…”

Section: Graphene Quantum Dots and Related Nanomaterialsmentioning

confidence: 99%

Carbon nanomaterials: multi-functional agents for biomedical fluorescence and Raman imaging

2015

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Carbon based nanomaterials have emerged over the last few years as important agents for biomedical fluorescence and Raman imaging applications. These spectroscopic techniques utilize either fluorescently labelled carbon nanomaterials or the intrinsic photophysical properties of the carbon nanomaterial. In this review article we present the utilization and performance of several classes of carbon nanomaterials, namely carbon nanotubes, carbon nanohorns, carbon nanoonions, nanodiamonds and different graphene derivatives, which are currently employed for in vitro as well as in vivo imaging in biology and medicine. A variety of different approaches, imaging agents and techniques are examined and the specific properties of the various carbon based imaging agents are discussed. Some theranostic carbon nanomaterials, which combine diagnostic features (i.e. imaging) with cell specific targeting and therapeutic approaches (i.e. drug delivery or photothermal therapy), are also included in this overview.

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Super-resolution fluorescence imaging of biocompatible carbon dots

Cited by 102 publications

References 22 publications

One-step, green, and economic synthesis of water-soluble photoluminescent carbon dots by hydrothermal treatment of wheat straw, and their bio-applications in labeling, imaging, and sensing

One-step, green, and economic synthesis of water-soluble photoluminescent carbon dots by hydrothermal treatment of wheat straw, and their bio-applications in labeling, imaging, and sensing

Carbon “Quantum” Dots for Fluorescence Labeling of Cells

Carbon nanomaterials: multi-functional agents for biomedical fluorescence and Raman imaging

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