Super long-term glycemic control in diabetic rats by glucose-sensitive LbL films constructed of supramolecular insulin assembly

Luo, Jun; Cao, Shuqin; Chen, Xingyu; Liu, Shuning; Tan, Hong; Wu, Wei; Li, Jianshu

doi:10.1016/j.biomaterials.2012.08.041

Cited by 75 publications

(62 citation statements)

References 46 publications

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“…31 The electrostatically anchored multilayer structure provided a controlled release of the loading cargo, which is pivotal for local drug delivery in order to obtain a sustained effect. [32][33][34] H1299 originates from epithelial lung cancer and has a prominent ability of endocytosis. Therefore, it is an ideal cell line for validating CS parameters for siRNA delivery.…”

Section: Discussionmentioning

confidence: 99%

Chitosan/siRNA functionalized titanium surface via a layer-by-layer approach for in vitro sustained gene silencing and osteogenic promotion

Song¹,

Song²,

Yang³

et al. 2015

IJN

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Titanium surface modification is crucial to improving its bioactivity, mainly its bone binding ability in bone implant materials. In order to functionalize titanium with small interfering RNA (siRNA) for sustained gene silencing in nearby cells, the layer-by-layer (LbL) approach was applied using sodium hyaluronate and chitosan/siRNA (CS/siRNA) nanoparticles as polyanion and polycation, respectively, to build up the multilayered film on smooth titanium surfaces. The CS/siRNA nanoparticle characterization was analyzed first. Dynamic contact angle, atomic force microscopy, and scanning electron microscopy were used to monitor the layer accumulation. siRNA loaded in the film was quantitated and the release profile of film in phosphate-buffered saline was studied. In vitro knockdown effect and cytotoxicity evaluation of the film were investigated using H1299 human lung carcinoma cells expressing green fluorescent protein (GFP). The transfection of human osteoblast-like cell MG63 and H1299 were performed and the osteogenic differentiation of MG63 on LbL film was analyzed. The CS/siRNA nanoparticles exhibited nice size distribution. During formation of the film, the surface wettability, topography, and roughness were alternately changed, indicating successful adsorption of the individual layers. The scanning electron microscope images clearly demonstrated the hybrid structure between CS/siRNA nanoparticles and sodium hyaluronate polymer. The cumulated load of siRNA increased linearly with the bilayer number and, more importantly, a gradual release of the film allowed the siRNA to be maintained on the titanium surface over approximately 1 week. In vitro transfection revealed that the LbL film-associated siRNA could consistently suppress GFP expression in H1299 without showing significant cytotoxicity. The LbL film loading with osteogenic siRNA could dramatically increase the osteogenic differentiation in MG63. In conclusion, LbL technology can potentially modify titanium surfaces with specific gene-regulatory siRNAs to enhance biofunction.

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Section: Discussionmentioning

confidence: 99%

Chitosan/siRNA functionalized titanium surface via a layer-by-layer approach for in vitro sustained gene silencing and osteogenic promotion

Song¹,

Song²,

Yang³

et al. 2015

IJN

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show abstract

“…Luo and co-workers combined the SIA in Layer by Layer (LbL) films in order to obtain an insulin reservoir with very tight control of the molecule release. Subcutaneous implants of SIA structured in LbL films were applied to diabetic rats allowing the control of the glucose levels in an accurate manner during 295 days (125) . Similarly, analogues of the GnRH can be used for the control of numerous sex steroid dependent pathophysiologies.…”

Section: Hydrogels For Nerve Tissue Regenerationmentioning

confidence: 99%

“…LbL films Long term control of glucose levels (125) GnRH analogues Amyloid fibers Treatment of sex-steroid dependent pathophysiologies (126) Retinoic Acid -Κ-Casein RA functionalizing amyloid K casein hydrogel Neuronal differentiation (136) IGF-1 Functionalizing amyloid fibrils Improve cardiac myocyte survival and growth in (137) infarctation rat models Bacterial inclusion bodies Functionalizing 2D and 3D cell culture scaffolds Show promise as protein drug delivery platform (127) IKVAV or RGD Functionalizing PA fibers In vitro and in vivo neurite growth stimulation (142) IKVAV Functionalizing PA fibers Restores cognitive impairment in Alzheimer's disease (143) …”

Section: Hydrogels For Nerve Tissue Regenerationmentioning

confidence: 99%

Integrating Mechanical and Biological Control of Cell Proliferation Through Bioinspired Multieffector Materials

Seras‐Franzoso

Tatkiewicz

Vázquez

et al. 2015

Nanomedicine (Lond.)

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In nature, cells respond to complex mechanical and biological stimuli whose understanding is required for tissue construction in regenerative medicine. However, the full replication of such bimodal effector networks is far to be reached. Engineering substrate roughness and architecture allows regulating cell adhesion, positioning, proliferation, differentiation and survival, and the external supply of soluble protein factors (mainly growth factors and hormones) has been long applied to promote growth and differentiation. Further, bio-inspired scaffolds are progressively engineered as reservoirs for the in situ sustained release of soluble protein factors from functional topographies. We review here how research progresses towards the design of integrative, holistic scaffold platforms based on the exploration of individual mechanical and biological effectors and their further combination.

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“…The worldwide prevalence of diabetes is recently made in the fi eld of pharmacy, biology, biochemistry, and biomaterials, [5][6][7][8][9][10][11] to realize the desired glycemic control effect by delivering an accurate dose of insulin in response to glucose level changes. Among them, one expedient strategy is to develop glucose-sensitive materials to be utilized in a self-regulated insulin delivery system, [ 12,13 ] which has a promising future within the application of diabetes treatment.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Y‐Shaped Copolymers Containing Phenylborate Ester and Biodegradable Poly(lactic acid) Blocks and Their Glucose‐Sensitive Behavior for Controlled Insulin Release

Zhao

Zhang

Yao

et al. 2014

Macro Chemistry & Physics

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This study is devoted to developing amphiphilic, biodegradable, Y-shaped block polymers, which can self-assemble to form nanoparticles, as a glucose-sensitive drug carrier. Y-shaped poly(ethylene glycol)-block -[poly(lactic acid)-block -poly (2-phenylborate esters-1,3-dioxane-5-methyl) methylacrylate)] 2 (MPEG-(PLA-block -PPBDMMA) 2 ) polymers are constructed via a combination of ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP) with MPEG-(OH) 2 , having double initiation endings, as starting material. These Y-shaped block polymers can disperse in an aqueous medium and self-assemble into micellar aggregates with a spherical core-shell structure. Dynamic laser scattering (DLS), zeta potential measurements, and transmission electron microscopy (TEM) show the solution properties of these polymeric micelles with or without insulin loading. Compared with MPEG-PLA-block -PPBDMMA linear polymer, in vitro insulin release at different glucose concentrations, revealed by the fl uorescence technique, shows that the studied Y-shaped polymers have intelligent glucoseconcentration-dependent manner of insulin release at pH 7.4 and 37 °C. The bulk swelling phenomenon of the polymeric micelles formed from the Y-shaped polymers is detected by DLS in the process of their glucose-responsiveness. Such a Y-shaped glucose-responsive polymer is a promising candidate that holds great potential in the treatment of diabetes. predicted to drastic increase to 440 million by 2030, [ 1 ] and it has become one of the most important health concerns we ought to confront. Currently, the common treatment involves frequent blood glucose monitoring and multi ple daily insulin injections. [ 2 ] This provides some control of the diabetic state, but this is far from the physiologic regulation achieved by a normally functioning pancreas and results in unexpected fl uctuations in blood glucose and long-term complications. [ 3,4 ] Great effort has been 0 1 0 2 0 3 0 4 0 5 0 0 20 40 60 80 100 Cumulative Release % Time (h) [Glc]= 0 mg/mL [Glc]=1.0 mg/mL [Glc]=3.0 mg/mL [Glc]=5.0 mg/mL

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Super long-term glycemic control in diabetic rats by glucose-sensitive LbL films constructed of supramolecular insulin assembly

Cited by 75 publications

References 46 publications

Chitosan/siRNA functionalized titanium surface via a layer-by-layer approach for in vitro sustained gene silencing and osteogenic promotion

Chitosan/siRNA functionalized titanium surface via a layer-by-layer approach for in vitro sustained gene silencing and osteogenic promotion

Integrating Mechanical and Biological Control of Cell Proliferation Through Bioinspired Multieffector Materials

Synthesis of Y‐Shaped Copolymers Containing Phenylborate Ester and Biodegradable Poly(lactic acid) Blocks and Their Glucose‐Sensitive Behavior for Controlled Insulin Release

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