Sunlight decreased genotoxicity of azadirachtin on root tip cells of Allium cepa and Eucrosia bicolor

Kwankua, W.; Sengsai, Supanyika; Kuleung, Chatuporn; Euawong, Nongnuch

doi:10.1016/j.ecoenv.2010.04.001

Cited by 18 publications

(12 citation statements)

References 25 publications

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“…The most common aberrations observed in all treatments of T. erecta and T. patula were the aberrations in anaphase cells, metaphase and telophase (Table 6). Reduction in the number of dividing cells was observed in the majority of the treatments performed with the different extracts of T. erecta and T. patula, indicating an inhibitory effect on mitosis of A. cepa root cells, with a higher incidence of aberrant cells (Tables 5 and 6), as found in the work of Soliman (2001) and Kwankua et al (2010), who found similar results with extracts of neem (Azadirachta indica). Knoll et al (2006) demonstrated the genotoxic potential of Pterocaulon polystachyum by mean of the A. cepa test, detecting the cell division inhibition capacity with increasing concentration of the aqueous extracts.…”

Section: Discussionsupporting

confidence: 82%

Phytotoxicity of Tagetes erecta L. and Tagetes patula L. on plant germination and growth

Santos

Mecina

et al. 2015

South African Journal of Botany

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This study evaluated the phytotoxic potential and antioxidant activity of Tagetes patula and Tagetes erecta, and total phenols and flavonoids of the extracts were quantified. Laboratory bioassays for both pre-and postemergence were performed in Lactuca sativa L. seeds and in the Allium cepa seeds test. The antioxidant activity was evaluated by DPPH radical scavenging and reducing power of iron, besides total phenols and flavonoids quantification in the extracts. Thus, was observed a reduction in the mitotic index when in compared with the negative control. Was observed also a reduction the germination and development of tested seedlings and was verified a considerable antioxidant potential and presence of flavonoids and phenolic compounds in the extracts. According to these results, it is possible to conclude that T. erecta and T. patula have phytotoxic compounds that may enhance and expand their use in the management of organic agriculture, mainly in vegetables.

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Section: Discussionsupporting

confidence: 82%

Phytotoxicity of Tagetes erecta L. and Tagetes patula L. on plant germination and growth

Santos

Mecina

et al. 2015

South African Journal of Botany

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“…Research have shown that inhibition of root growth of A. cepa is probably related to reduction of mitotic index (Akinboro and Bakare, 2007;Da Silva et al, 2012). In similarly way, reduction in number of dividing cells was observed in treatments performed with different extracts of T. procumbens, indicating an inhibitory effect in A. cepa root cells mitosis, with the highest incidence of aberrant cells (Table 1 and 2), as demonstrated in the work done by Kwankua et al (2010) and Timothy et al (2014). In accordance with reduction in mitotic index and increased frequency of aberrant cells, were found changes in treatments with T. procumbens in death index (Table 1) Time (min) WL = 614.00 nm WL = 332.00 nm Knoll et al (2006) evidenced potential genotoxic activity in Pterocaulon polystachyum through A. cepa test, detecting an inhibitory capacity in cell division related to increasing concentrations of aqueous extracts.…”

Section: Discussionsupporting

confidence: 62%

Cytotoxicity and Genotoxicity of Tridax procumbens L. in Allium cepa

Mecina¹,

Montenoti²,

Moraes³

et al. 2016

Research J. of Medicinal Plant

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With increased consumption of medicinal plants in alternative treatments and the search for new compounds with biological activity, the need arose to investigate the toxic, carcinogenic and teratogenic potential of these compounds. Among many species, Tridax procumbens stands out for being a plant commonly used in folk medicine, finding varying reports of its use. This study was conducted to investigate cytotoxic and genotoxic effects of ethanolic, aqueous and hydroethanolic extracts of this species and a phytochemical screening of different classes present in extract by HPLC-PAD. Allium cepa seedlings were exposed to extracts for 48 h. After this period, the seedlings were replaced in distilled water. Then the roots were hydrolyzed in hydrochloric acid (HCl) 1 N and stained with Schiff reactive. The roots were placed on slides, then was added a drop of 2% acetic carmine and covered with coverslips and were observed 5000 cells per treatment. It was found that different extracts led to a reduction in mitotic index when compared with negative control and still presented changes in death rate. In this context, results suggest that extracts of T. procumbens have inhibitory effect on mitosis and mutagens on cell division in root meristematic cells of A. cepa.

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“…Additionally, the highest AI value was obtained for the treatment with NC_20 at the highest concentration (20 mg/mL), similar to the findings of Kwankua et al . 43 .…”

Section: Resultsmentioning

confidence: 99%

Nanocapsules Containing Neem (Azadirachta Indica) Oil: Development, Characterization, And Toxicity Evaluation

Pasquoto-Stigliani

Campos

Oliveira

et al. 2017

Sci Rep

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In this study, we prepared, characterized, and performed toxicity analyses of poly(ε-caprolactone) nanocapsules loaded with neem oil. Three formulations were prepared by the emulsion/solvent evaporation method. The nanocapsules showed a mean size distribution around 400 nm, with polydispersity below 0.2 and were stable for 120 days. Cytotoxicity and genotoxicity results showed an increase in toxicity of the oleic acid + neem formulations according to the amount of oleic acid used. The minimum inhibitory concentrations demonstrated that all the formulations containing neem oil were active. The nanocapsules containing neem oil did not affect the soil microbiota during 300 days of exposure compared to the control. Phytotoxicity studies indicated that NC_20 (200 mg of neem oil) did not affect the net photosynthesis and stomatal conductance of maize plants, whereas use of NC_10 (100:100 of neem:oleic acid) and NC_15 (150:50 of neem:oleic acid) led to negative effects on these physiological parameters. Hence, the use of oleic acid as a complement in the nanocapsules was not a good strategy, since the nanocapsules that only contained neem oil showed lower toxicity. These results demonstrate that evaluation of the toxicity of nanopesticides is essential for the development of environmentally friendly formulations intended for applications in agriculture.

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Sunlight decreased genotoxicity of azadirachtin on root tip cells of Allium cepa and Eucrosia bicolor

Cited by 18 publications

References 25 publications

Phytotoxicity of Tagetes erecta L. and Tagetes patula L. on plant germination and growth

Phytotoxicity of Tagetes erecta L. and Tagetes patula L. on plant germination and growth

Cytotoxicity and Genotoxicity of Tridax procumbens L. in Allium cepa

Nanocapsules Containing Neem (Azadirachta Indica) Oil: Development, Characterization, And Toxicity Evaluation

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