Sulfur-containing amino acid supplementation to gilts from late pregnancy to lactation altered offspring’s intestinal microbiota and plasma metabolites

Azad, Md. Abul Kalam; Liu, Gang; Peng, Bin; Kong, Xiangfeng; Guan, Guijian; Yin, Yulong

doi:10.1007/s00253-019-10302-6

Cited by 20 publications

(14 citation statements)

References 55 publications

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“…Thus, the observed higher abundance of Spirochaetaes in the amylose/amylopectin ratio groups could be associated with the level of fiber in the diet. It has been widely reported that Proteobacteria consists of well-known pathogens such as Escherichia coli and Salmonell ( 43 ), while Verrucomicrobia consists of commensal bacteria such as Akkermansia ( 39 ). The present results showed that feeding a diet containing 50% high amylose decreased Proteobacteria and increased Verrucomicrobia abundances in cecal mucosa, showing that enrichments of commensal bacteria, which contributes to developing a healthier pattern of cecal environment.…”

Section: Discussionmentioning

confidence: 99%

Dietary Amylose/Amylopectin Ratio Modulates Cecal Microbiota and Metabolites in Weaned Goats

et al. 2021

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Increasing the ratio of amylose in the diet can increase the quantity of starch that flows to the large intestine for microbial fermentation. This leads to the alteration of microbiota and metabolite of the hindgut, where the underlying mechanism is not clearly understood. The present study used a combination of 16S amplicon sequencing technology and metabolomics technique to reveal the effects of increasing ratios of amylose/amylopectin on cecal mucosa- and digesta-associated microbiota and their metabolites in young goats. Twenty-seven Xiangdong black female goats with average body weights (9.00 ± 1.12 kg) were used in this study. The goats were randomly allocated to one of the three diets containing starch with 0% amylose corn (T1), 50% high amylose corn (T2), and 100% high amylose corn (T3) for 35 days. Results showed that cecal valerate concentration was higher (P < 0.05) in the T2 group than those in the T1 and T3 groups. The levels of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 were decreased (P < 0.05) in cecal tissue while IL-10 was increased (P < 0.05) in the T2 group when compared with T1 or T3 groups. At the phylum level, the proportion of mucosa-associated Spirochaetes was increased (P < 0.05), while Proteobacteria was deceased by feeding high amylose ratios (P < 0.05). The abundance of Verrucomicrobia was decreased (P < 0.05) in the T3 group compared with the T1 and T2 groups. The abundance of digesta-associated Firmicutes was increased (P < 0.05) while Verrucomicrobia and Tenericutes were deceased (P < 0.05) with the increment of amylose/amylopectin ratios. The LEfSe analysis showed that a diet with 50% high amylose enriched the abundance of beneficial bacteria such as Faecalibacterium and Lactobacillus in the digesta and Akkermansia in the mucosa compared with the T1 diet. The metabolomics results revealed that feeding a diet containing 50% high amylose decreased the concentration of fatty acyls-related metabolites, including dodecanedioic acid, heptadecanoic acid, and stearidonic acid ethyl ester compared with the T1 diet. The results suggested that a diet consisting of 50% high amylose could maintain a better cecal microbiota composition and host immune function.

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Section: Discussionmentioning

confidence: 99%

Dietary Amylose/Amylopectin Ratio Modulates Cecal Microbiota and Metabolites in Weaned Goats

et al. 2021

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“…Several studies have demonstrated that the gut microbial butanoate metabolic pathway exerts beneficial effects on body weight of livestock due to its potential impact on increasing energy intake, improving intestinal histomorphometric characteristics (e.g., villi height and crypt depth), and modulating the immune system (Ndou et al, 2018;Che et al, 2019;Jacquier et al, 2019). Similar to the abovementioned cysteine, methionine is crucial in maintaining the functions of intestinal cells by regulating the redox status (Azad et al, 2019). Thus, enhanced methionine metabolism in the gut microbial communities contributed to bovine body weight gain (Jiao et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Effects of Gut Microbiome and Short-Chain Fatty Acids (SCFAs) on Finishing Weight of Meat Rabbits

Fang

Chen

et al. 2020

Front. Microbiol.

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Understanding how the gut microbiome and short-chain fatty acids (SCFAs) affect finishing weight is beneficial to improve meat production in the meat rabbit industry. In this study, we identified 15 OTUs and 23 microbial species associated with finishing weight using 16S rRNA gene and metagenomic sequencing analysis, respectively. Among these, butyrate-producing bacteria of the family Ruminococcaceae were positively associated with finishing weight, whereas the microbial taxa related to intestinal damage and inflammation showed opposite effects. Furthermore, interactions of these microbial taxa were firstly found to be associated with finishing weight. Gut microbial functional capacity analysis revealed that CAZymes, such as galactosidase, xylanase, and glucosidase, could significantly affect finishing weight, given their roles in regulating nutrient digestibility. GOs related to the metabolism of several carbohydrates and amino acids also showed important effects on finishing weight. Additionally, both KOs and KEGG pathways related to the membrane transportation system and involved in aminoacyl-tRNA biosynthesis and butanoate metabolism could act as key factors in modulating finishing weight. Importantly, gut microbiome explained nearly 11% of the variation in finishing weight, and our findings revealed that a subset of metagenomic species could act as predictors of finishing weight. SCFAs levels, especially butyrate level, had critical impacts on finishing weight, and several finishing weight-associated species were potentially contributed to the shift in butyrate level. Thus, our results should give deep insights into how gut microbiome and SCFAs influence finishing weight of meat rabbits and provide essential knowledge for improving finishing weight by manipulating gut microbiome.

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“…Research on fetal programming has been conducted on dairy cattle (30), sheep (31,32), and non-ruminants (33)(34)(35), with a particular focus on nutrient manipulation during late pregnancy and its impact on the fetus and postnatal development of the offspring. Therefore, it was hypothesized that maternal supplementation of late gestation dairy cows with ruminally protected Lys (RPL), RPM, or the two in combination would, in addition to benefitting the cows themselves, increase the supply of metabolizable Met and Lys and other essential AAs that reach the calf through uteroplacental transfer, thereby aiding fetal development, contributing to increased bovine colostrum IgG, maximizing passive immunity transfer from cow to calf via colostrum, and enhancing the health status of calves.…”

Section: Introductionmentioning

confidence: 99%

Maternal Supply of Ruminally-Protected Lysine and Methionine During Close-Up Period Enhances Immunity and Growth Rate of Neonatal Calves

Wang

Elsaadawy

et al. 2021

Front. Vet. Sci.

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The objective of this study was to evaluate the effect of supplying ruminally-protected lysine (RPL), methionine (RPM), or the two in combination (RPML) to transition dairy cows on the immunity and performance of their offspring. Eighty heifer calves (n = 20 calves per group) were assigned to four treatments based on their dam diet; basal diet (CON), a basal diet with lysine [RPL, 0.33% of dry matter (DM)], a basal diet with methionine (RPM, 0.16% DM), or with the combination (RPML). Calves were fed colostrum from their dams within 2 h of birth. Calves were then fed milk only (d 2–22), a combination of milk and milk replacer (d 23–25), and milk replacer (d 25–60). Starter feed was fed to the calves twice daily after liquid feeding. Calves blood samples were collected after calving on 0, 12, 24, and 48 h and 5 and 7 d after birth. Data were analyzed by SAS software v9.4. Providing ruminally-protected amino acids (RPAA) to transition cows improved colostrum quality compared to the CON (Brix; P < 0.01). Serum total protein concentrations were higher in calves from supplemented cows than in calves from unsupplemented cows (P < 0.01). Calves born to dams in the RPM, RPL, and RPML groups had higher plasma immunoglobulin G (IgG) concentrations 0, 12, 24, and 48 h and 7 d after birth than those born to dams in the CON group (P < 0.05). The percentage of calves with adequate passive immunity transfer was increased with RPM and RPL or the two in combination (P < 0.01). However, there was no difference in the percentage of calves with adequate passive immunity transfer between the RPM and RPL groups (P = 0.21). Calves from cows that receive supplemental RPAA have a greater average daily gain (ADG) than those born to cows in the CON group (P < 0.01). These results indicate that maternal supplementation with RPM or RPL or the two in combination during the periparturient period could be an alternative strategy to improve the performance of calves, especially in accelerated growth programs in calves.

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Sulfur-containing amino acid supplementation to gilts from late pregnancy to lactation altered offspring’s intestinal microbiota and plasma metabolites

Cited by 20 publications

References 55 publications

Dietary Amylose/Amylopectin Ratio Modulates Cecal Microbiota and Metabolites in Weaned Goats

Dietary Amylose/Amylopectin Ratio Modulates Cecal Microbiota and Metabolites in Weaned Goats

Effects of Gut Microbiome and Short-Chain Fatty Acids (SCFAs) on Finishing Weight of Meat Rabbits

Maternal Supply of Ruminally-Protected Lysine and Methionine During Close-Up Period Enhances Immunity and Growth Rate of Neonatal Calves

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