Sulfate reduction by a syntrophic propionate-oxidizing bacterium

Kuijk, B.L.M. van; Stams, A.J.M.

doi:10.1007/bf00874139

Cited by 41 publications

(28 citation statements)

References 22 publications

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“…hungateii, strain MPOBT had a growth rate on propionate of approximately 0.17 d-l. In pure culture, lower growth rates were observed; 0.09 d-' during fermentative growth on fumarate and 0.024 d-' during growth on propionate and sulfate (15). Both c-and b-type cytochromes as well as the menaquinones MK-6 and MK-7 were present in fumarate-grown cells.…”

Section: Physiological Characterizationmentioning

confidence: 92%

“…A mesophilic bacterium (strain MPOB') enriched by us on propionate was able to ferment fumarate to succinate and carbon dioxide without a syntrophic partner (12). This strain could oxidize propionate by using fumarate or sulfate as electron acceptors (12,15 pionate by the use of HS-CoA transferase (1 1). 16s rRNA sequence analysis of S. wolinii, S. pfennigii, strain HP1.l and strain MPOBT revealed that these syntrophic bacteria are closely related and belong to the delta subclass of Proteobacteria (3,4,19).…”

Section: Introductionmentioning

confidence: 99%

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Syntrophobacter fumaroxidans sp. nov., a syntrophic propionate-degrading sulfate-reducing bacterium

Harmsen

Kuijk²,

Plugge³

et al. 1998

International Journal of Systematic Bacteriology

192

125

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A syntrophic propionate-oxidizing bacterium, strain MPOBT, was isolated from a culture enriched from anaerobic granular sludge. It oxidized propionate syntrophically in co-culture with the hydrogen-and formate-utilizing Methanospirillurn hungateii, and was able to oxidize propionate and other organic compounds in pure culture with sulfate or fumarate as the electron acceptor. Additionally, it fermented f umarate. 16s rRNA sequence analysis revealed a relationship with Syn trophobacter wolinii and Syn trophobacter pfennigii. The G+C content of i t s DNA was 60.6 mol%, which is in the same range as that of other Syntrophobacter species. DNA-DNA hybridization studies showed less than 26% hybridization among the different genomes of Syntrophobacter species and strain MPOBT. This justifies the assignment of strain MPOBT to the genus Syntrophobacter as a new species. The name Syntrophobacter fumaroxidans is proposed; strain MPOBT (= DSM 10017T) is the type strain. INTRODUCTIONFor a long time, Syntrophobacter niolinii was the only described bacterium which could oxidize propionate syntrophically in co-culture with the hydrogen-consuming Desulfovibrio G11 (1). Several methanogenic syntrophic co-cultures were enriched, but obtaining defined co-cultures remained difficult. S. wolinii was only recently obtained in pure culture, and found to be able to grow on pyruvate or on propionate and sulfate (1 7). Two related syntrophic propionate-oxidizing bacteria, Sjwtrophobacter pfennigii, previously known as KOPROPl, and strain HP1.l were isolated with propionate and sulfate (1 8,19). A mesophilic bacterium (strain MPOB') enriched by us on propionate was able to ferment fumarate to succinate and carbon dioxide without a syntrophic partner (12). This strain could oxidize propionate by using fumarate or sulfate as electron acceptors (12,15 pionate by the use of HS-CoA transferase (1 1). 16s rRNA sequence analysis of S. wolinii, S. pfennigii, strain HP1.l and strain MPOBT revealed that these syntrophic bacteria are closely related and belong to the delta subclass of Proteobacteria (3,4,19). Remarkably, it was observed that another bacterium was related to this group : Desulforhabdus amnigenus, a sulfate-reducing bacterium which is not able to grow syntrophically on propionate (10).Recently, we obtained a pure culture of strain MPOBT. Its morphological and physiological characterization are presented here, and its taxonomic position within the genus Syntrophobacter is discussed. University, The Netherlands). A previously described bicarbonate-buffered medium was used for isolation and cultivation (1 2). For isolation of strain MPOB' the roll-tube-dilution method ( 5 ) and direct dilution series in liquid media with fumarate as carbon and energy sources were used. Purity was checked by growth in Wilkins-Chalgren anaerobe broth (Oxoid), and in media containing 1 % yeast extract and 20 mM glucose, and by microscopy. METHODSPhylogeny and DNA analysis. Phylogenetic analysis of the strain has been described previously (4, 10). The nucleo...

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Section: Physiological Characterizationmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Syntrophobacter fumaroxidans sp. nov., a syntrophic propionate-degrading sulfate-reducing bacterium

Harmsen

Kuijk²,

Plugge³

et al. 1998

International Journal of Systematic Bacteriology

192

125

View full text Add to dashboard Cite

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“…(j) At day 43 of the experiment reported by Kuijk and Stams (1995, their fig. 1); the growth medium has a pH of 7 and contains the following ions: 5.6 mmolal ammonium, 47.6 mmolal bicarbonate, 0.8 mmolal Ca 2ϩ , 13.2 mmolal Cl Ϫ , 3.0 mmolal K ϩ , 0.5 mmolal Mg 2ϩ , 62.7 mmolal Na ϩ , and 6.0 mmolal phosphate.…”

Section: Available Energymentioning

confidence: 98%

Energy conservation of anaerobic respiration

Jin

2012

American Journal of Science

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Microbes save energy from the environment by synthesizing ATP. Understanding microbial energy conservation is important for both theoretical and practical applications. This paper focuses on the common metabolism of anoxic environments-ferric iron respiration, sulfate respiration, and methanogenesis-and analyzes microbial energy conservation on the basis of the thermodynamics as well as physiological models of respiratory reactions. The results of the analysis show that iron respiration synthesizes 1 to 4 ATPs by transferring eight electrons from H 2 , acetate, lactate, and ethanol to ferric minerals; sulfate respiration makes 0.25 to more than 3 ATPs by transferring eight electrons from the same suite of electron donors to sulfate; methanogenesis yields 0 to 1 ATP by oxidizing four H 2 and by disproportionating one acetate. The ATP yields are compared to growth yields and energy thresholds of anaerobic metabolism to explore the impact of energy conservation. Specifically, energy conservation controls microbial growth: in geochemical systems, respiring microbes synthesize up to 5 g biomass per mole of ATP. Energy conservation also requires the environment to supply chemical energy at quantities greater than the energy saved by microbes. These results unify our view of microbial metabolism, and can be applied to evaluating the occurrence and significance of microbial life in natural environments.

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“…The ability to use sulphate as a terminal electron acceptor is a characteristic that strain TPO shares with other mesophilic, nonsporulating, syntrophic, propionate-oxidizers (Wallrabenstein et al, 1995 ;Van Kuijk & Stams, 1995). On the basis of the enzyme measurements shown in Table 3, it seems that strain TPO uses the methylmalonylCoA pathway for syntrophic propionate oxidation.…”

Section: Comparison Of Strain Tpo With Other Syntrophic Organismsmentioning

confidence: 99%