Sugarcane pre-sprouted seedlings: A novel method for sugarcane establishment

Otto, Rafael; Machado, Bianca A.; Silva, Ana Carolina Melo da; Castro, Sérgio Gustavo Quassi de; Lisboa, Izaías Pinheiro

doi:10.1016/j.fcr.2021.108336

Cited by 10 publications

(9 citation statements)

References 45 publications

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“…Different SIGs contain antagonistic mechanism resisting PTGS. Hence transgene stability is not compromised and remains intact as reported in previous studies [32][33][34] . Gene silencing was the potential threat in present work as it exploited pUbi promoter, a partial target of gene silencing 35 but not a single observation related to silencing event, transcriptional or post transcriptional gene silencing was seen.…”

Section: Discussionsupporting

confidence: 60%

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

Awan

Ali

Iqbal

et al. 2022

Sci Rep

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Enhancement of sugar contents and yielding healthful sugar products from sugarcane demand high profile scientific strategies. Previous efforts to foster manipulation in metabolic pathways or triggering sugar production through combating abiotic stresses fail to yield high sugar recovery in Saccharum officinarum L. Novel sucrose isomers trehalulose (TH) and isomaltulose (IM) are naturally manufactured in microbial sources. In pursuance of novel scientific methodology, codon optimized sucrose isomerase gene, Trehalulose synthase gene II(CEMB-SIG2) cloned under dual combined stem specific constitutive promoters in pCAMBIA1301 expression vector integrated with Vacuole targeted signal peptide (VTS) to concentrate gene product into the vacuole. The resultant mRNA expression obtained by Real Time PCR validated extremely increased transgene expression in sugarcane culms than leaf tissues. Overall sugar estimation from transgenic sugarcane lines was executed through refractometer. HPLC based quantifications of Trehalulose (TH) alongside different internodes of transgenic sugarcane confirmed the enhancement of boosted sugar concentrations in mature sugarcane culms. Trehalulose synthase gene II receptive sugarcane lines indicated the unprecedented impressions of duly combined constitutive stem regulated promoters. Transgenic sugarcane lines produce highest sugar recovery percentages, 14.9% as compared to control lines (8.5%). The increased sugar recovery percentage in transgenic sugarcane validated the utmost performance and expression of ThSyGII gene .High Profile Liquid chromatography based sugar contents estimation of Trehalulose (TH) and Isomaltulose (IM) yielded unprecedented improvement in the whole sugar recovery percentage as compared to control lines.⁠

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Section: Discussionsupporting

confidence: 60%

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

Awan

Ali

Iqbal

et al. 2022

Sci Rep

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“…However, PSS have reduced nutrient and water reserves compared to the conventional method and, consequently, are more susceptible to abiotic stresses, especially water restriction [17]. Therefore, water supplementation is recommended after transplanting PSS from plant seedling nurseries to the field since there is high plant mortality in planting seasons with low rainfall [10,17,18].…”

Section: Introductionmentioning

confidence: 99%

Plant Growth-Promoting Bacteria Enhance Survival, Growth, and Nutritional Content of Sugarcane Propagated through Pre-Sprouted Seedlings under Water Deficit

Almeida,

Santos,

Nogueira

et al. 2024

Agriculture

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Sugarcane plays a crucial role in global sugar and ethanol production. Conventionally, sugarcane propagation involves planting billets. However, Brazilian researchers have introduced the innovative pre-sprouted seedlings (PSS) method, widely used in the MEIOSI (Simultaneously Occurring Interrotational Method) system. Although PSS has several advantages over the conventional method, its sensitivity to water scarcity is a challenge. This study aimed to evaluate the survival and growth of PSS inoculated with Bacillus licheniformis and Bacillus subtilis (PGPB) under different water regimes. The experiment was conducted in the field in a randomized block in strips (split-block) using a 2 × 4 factorial scheme consisting of two inoculation conditions (with and without PGPB) and four water regimes (WR) (0%, 33%, 66%, and 100% of the ideal irrigation). PGPB increased PSS survival (4.17%) and water use efficiency (185.10% under the 0% WR). In addition, inoculation increased root (12.5%, 7.7%, and 16.2% for surface area, area projection, and root volume, respectively) and shoot growth. PGPB also increased nutrient uptake, such as N (60.8% and 18.3% under 0% and 66% WR), P (on average 11.7%), Mn (27.6% and 28.7% under 66% and 100% WR), Zn (111.4% under 0% WR), and Cu accumulation (27.17%), which provided a higher number of stalks per meter and, therefore, a higher multiplication rate in the MEIOSI system. Inoculation proved to be a promising alternative for PSS establishing under water restriction.

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“…Recent studies indicate that the adjustment of the fertilizer rate according to the sugarcane harvest season (i.e., autumn, winter, or spring) promotes production gains ( Castro et al., 2022 ) and increases nutrient use efficiency by the plant ( Bowles et al., 2018 ; Congreves et al., 2021 ; Farzadfar et al., 2021 ). These increments are obtained when soil fertilization has been carried out under conditions of adequately high soil moisture, which favors nutrient availability for plant uptake ( Castro et al., 2022 ; Otto et al., 2022b ). However, under excessively high soil moisture and water logging conditions, nutrient losses might increase due to denitrification and leaching ( Otto et al., 2016 ; Bowles et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Fertilizer source and application method influence sugarcane production and nutritional status

Castro

Coelho²,

Castro³

et al. 2023

Front. Plant Sci.

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IntroductionThe contrasting weather conditions throughout the sugarcane harvest period in south-central Brazil (April to November) influence fertilization management in sugarcane ratoon.MethodsThrough field studies carried out over two cropping seasons, we aimed to compare the performance of sugarcane at sites harvested in the early and late periods of the harvest season as a function of fertilizer sources associated with application methods. The design used in each site was a randomized block in a 2 x 3 factorial scheme; the first factor consisted of fertilizer sources (solid and liquid), and the second factor consisted of application methods (above the straw, under the straw, and incorporated into the middle of the sugarcane row).ResultsThe fertilizer source and application method interacted at the site harvested in the early period of the sugarcane harvest season. Overall, the highest sugarcane stalk and sugar yields at this site were obtained with the incorporated application applying liquid fertilizer and under straw applying solid fertilizer, with increments of up to 33%. For the site harvested in the late period of the sugarcane harvest season, the liquid fertilizer promoted a 25% higher sugarcane stalk yield compared to the solid fertilizer in the crop season with low rainfall in the spring, while in the crop season with normal rainfall, there were no differences between treatments.DiscussionThis demonstrates the importance of defining fertilization management in sugarcane as a function of harvest time, thereby promoting greater sustainability in the production system

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Sugarcane pre-sprouted seedlings: A novel method for sugarcane establishment

Cited by 10 publications

References 45 publications

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

Plant Growth-Promoting Bacteria Enhance Survival, Growth, and Nutritional Content of Sugarcane Propagated through Pre-Sprouted Seedlings under Water Deficit

Fertilizer source and application method influence sugarcane production and nutritional status

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