Sucrose phosphate synthase (SPS), sucrose synthase (SUS) and their products in the leaves of Miscanthus × giganteus and Zea mays at low temperature

Bilska-Kos, Anna; Mytych, Jennifer; Suski, Szymon; Magoń, Justyna; Ochodzki, Piotr; Żebrowski, Jacek

doi:10.1007/s00425-020-03421-2

Cited by 47 publications

(38 citation statements)

References 61 publications

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“…It is possible that stress due to the supply of Cu 2+ and Ag + ions in the regeneration medium affected the status of cellulose primarily in a relatively more amorphic phase. In Miscanthus giganteum, a slight shift in the cellulose peak in this region of frequency has been observed as a result of mild chilling stress [63], which was associated with the formation of a more compact cellulose structure. The band at 700 cm −1 may be assigned not only to cellulose but also to the C-S stretching vibrations, which may be attributed to thiol or thioester functional groups.…”

Section: Discussionmentioning

confidence: 92%

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

Bednarek

Żebrowski

Orłowska

2020

IJMS

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Tissue culture is an essential tool for the regeneration of uniform plant material. However, tissue culture conditions can be a source of abiotic stress for plants, leading to changes in the DNA sequence and methylation patterns. Despite the growing evidence on biochemical processes affected by abiotic stresses, how these altered biochemical processes affect DNA sequence and methylation patterns remains largely unknown. In this study, the methylation-sensitive Amplified Fragment Length Polymorphism (metAFLP) approach was used to investigate de novo methylation, demethylation, and sequence variation in barley regenerants derived by anther culture. Additionally, we used Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy to identify the spectral features of regenerants, which were then analyzed by mediation analysis. The infrared spectrum ranges (710–690 and 1010–940 cm−1) identified as significant in the mediation analysis were most likely related to β-glucans, cellulose, and S-adenosyl-L-methionine (SAM). Additionally, the identified compounds participated as predictors in moderated mediation analysis, explaining the role of demethylation of CHG sites (CHG_DMV) in in vitro tissue culture-induced sequence variation, depending on the duration of tissue culture. The data demonstrate that ATR-FTIR spectroscopy is a useful tool for studying the biochemical compounds that may affect DNA methylation patterns and sequence variation, if combined with quantitative characteristics determined using metAFLP molecular markers and mediation analysis. The role of β-glucans, cellulose, and SAM in DNA methylation, and in cell wall, mitochondria, and signaling, are discussed to highlight the putative cellular mechanisms involved in sequence variation.

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Section: Discussionmentioning

confidence: 92%

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

Bednarek

Żebrowski

Orłowska

2020

IJMS

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“…There is still evidence that they play an important role in stress resistance. For example, Glyma.09g073600 and Glyma.18g108100 regulate sucrose-related enzymes to increase resistance to low temperature stress [42]; Glyma.04g119700 regulates maltooligosyltrehalose under salt stress [43]; Glyma.12g054200 is considered to be a factor potentially involved in regulating the salt adaptation of α -galactosidase production [44]. In addition, Baud et al [45] and Santaniello et al [46] found that the Arabidopsis thaliana At3G43190 gene homologous to Glyma.09G073600, plays an important role in the regulation of sucrose synthase activity in plant sugar metabolism, whereas Chary et al [47] found that the At1G68020 homolog of Glyma.04G119700 affects Arabidopsis cellular morphology and plant structure via the activity of trehalose -6-phosphate synthase/phosphatase.…”

Section: Discussionmentioning

confidence: 99%

Exogenous application of melatonin may contribute to enhancement of soybean drought tolerance via its effects on glucose metabolism

Cao

Qin

Zhang

2021

Biotechnology & Biotechnological Equipment

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Sugar metabolism is among the most important metabolic processes in soybean; it not only affects growth and development, but also plays a role in drought resistance. As a plant biological regulator, exogenous melatonin is assumed to regulate sugar metabolism. Here, physiological and transcriptomic approaches were used to determine the effects of melatonin on physiological indices and key genes and pathways associated with soybean sugar metabolism under drought stress. In plants subjected to drought stress, melatonin treatment increased dry matter accumulation, reduced malondialdehyde content, and increased the content of metabolized sugars in leaves, thereby enhancing soybean yield. Transcriptome analysis revealed that the genes differentially expressed in plants subjected to melatonin treatment and drought stress were enriched in the KEGG "sucrose and starch metabolism" pathway related to glucose metabolism. Among the differentially expressed genes, we identified 12 significantly altered genes associated with glucose metabolism that played roles in regulating the activities of glucose metabolism-related enzymes. Collectively, our results indicated that melatonin can enhance the output of glucose metabolites and tolerance of soybean subjected to drought stress by regulating the genes associated with glucose metabolism and activation of the related enzymes. Our findings provide a detailed theoretical basis for analyzing the regulatory effects of melatonin on soybean sugar metabolism under conditions of drought stress.

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“…Sucrose phosphate synthase (SPS) catalyzes the conversion of fructose-6-phosphate and uridine diphosphate-glucose into sucrose-6phosphate. The activity and localization of these enzymes can be controlled by reversibility phosphorylation process controlled by calcium-dependent kinases (Bilska-Kos et al, 2020). In turn, sucrose-phosphate phosphatase (SPP) catalyzes the final step of the sucrose biosynthesis pathway.…”

Section: Reconstruction and Comparative Analysis Of Sucrose Metabolism Pathwaymentioning

confidence: 99%

Unraveling the Genome of a High Yielding Colombian Sugarcane Hybrid

et al. 2021

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Recent developments in High Throughput Sequencing (HTS) technologies and bioinformatics, including improved read lengths and genome assemblers allow the reconstruction of complex genomes with unprecedented quality and contiguity. Sugarcane has one of the most complicated genomes among grassess with a haploid length of 1Gbp and a ploidies between 8 and 12. In this work, we present a genome assembly of the Colombian sugarcane hybrid CC 01-1940. Three types of sequencing technologies were combined for this assembly: PacBio long reads, Illumina paired short reads, and Hi-C reads. We achieved a median contig length of 34.94 Mbp and a total genome assembly of 903.2 Mbp. We annotated a total of 63,724 protein coding genes and performed a reconstruction and comparative analysis of the sucrose metabolism pathway. Nucleotide evolution measurements between orthologs with close species suggest that divergence between Saccharum officinarum and Saccharum spontaneum occurred <2 million years ago. Synteny analysis between CC 01-1940 and the S. spontaneum genome confirms the presence of translocation events between the species and a random contribution throughout the entire genome in current sugarcane hybrids. Analysis of RNA-Seq data from leaf and root tissue of contrasting sugarcane genotypes subjected to water stress treatments revealed 17,490 differentially expressed genes, from which 3,633 correspond to genes expressed exclusively in tolerant genotypes. We expect the resources presented here to serve as a source of information to improve the selection processes of new varieties of the breeding programs of sugarcane.

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Sucrose phosphate synthase (SPS), sucrose synthase (SUS) and their products in the leaves of Miscanthus × giganteus and Zea mays at low temperature

Abstract: Main conclusion The changes in the expression of key sugar metabolism enzymes (SPS and SUS), sucrose content and arrangement of chloroplast starch may play a significant role in the cold response in M. giganteus and maize plants.

Cited by 47 publications

References 61 publications

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

Exogenous application of melatonin may contribute to enhancement of soybean drought tolerance via its effects on glucose metabolism

Unraveling the Genome of a High Yielding Colombian Sugarcane Hybrid

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