Successful pregnancy after oocyte activation by a calcium ionophore for a patient with recurrent intracytoplasmic sperm injection failure, with an assessment of oocyte activation and sperm centrosomal function using bovine eggs

Terada, Yukihiro; Hasegawa, Hisataka; Takahashi, Aiko; Ugajin, Tomohisa; Yaegashi, Nobuo; Okamura, Koji

doi:10.1016/j.fertnstert.2008.09.043

Cited by 40 publications

(26 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increase of cytosolic free Ca 2+ (due to burst from internal stores) induces EA during fertilization [5,38,39]. Further, CI increases cytosolic free Ca 2+ level [35] and induces EA in several mammalian species [11,14,16,32,[40][41][42]. In the present study, we used CI as a positive control and data suggest that CI induced morphological features characteristics of EA in a dose-dependent manner.…”

Section: Discussionmentioning

confidence: 64%

An insufficient increase of cytosolic free calcium level results postovulatory aging-induced abortive spontaneous egg activation in rat

Premkumar

Chaube

2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The present study was aimed to find out whether postovulatory aging-induced abortive spontaneous egg activation (SEA) is due to insufficient increase of cytosolic free Ca 2+ level. Methods Immature female rats (22-24 days old) were subjected to superovulation induction protocol. Eggs were collected 14, 17 and 19 h post-hCG surge to induce in vivo egg aging. The eggs were collected 14 h post-hCG surge and cultured in vitro for 3, 5 and 7 h to induce in vitro egg aging. The morphological changes, rate of abortive SEA, chromosomal status and cytosolic free Ca 2+ levels were analyzed. Results Postovulatory aging induced morphological features characteristics of abortive SEA in a time-dependent manner in vivo as well as in vitro. The extracellular Ca 2+ increased rate of abortive SEA during initial period of culture, while coaddition of a nifedipine (L-type Ca 2+ channel blocker) protected against postovulatory aging-induced abortive SEA. However, CI induced morphological features characteristics of egg activation (EA) in a dose-dependent manner. As compare to control, an increase of cytosolic free Ca 2+ level (1.42 times) induced abortive SEA, while further increase of cytosolic free Ca 2+ level (2.55 times) induced EA. Conclusion Our results show that an insufficient cytosolic free Ca 2+ level is associated with postovulatory aginginduced abortive SEA, while furthermore increase is required to induce EA in rat.

show abstract

Section: Discussionmentioning

confidence: 64%

An insufficient increase of cytosolic free calcium level results postovulatory aging-induced abortive spontaneous egg activation in rat

Premkumar

Chaube

2012

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Nevertheless, low fertilization or fertilization failures after ICSI occur in~4 % of ICSI cases [12,17], and the great majority of those cases show a consistent phenotype; namely, absence of egg activation [25]. ICSI failure is also observed in a disproportionate number of patients with globoozoospermia [17,26].…”

Section: Discussionmentioning

confidence: 99%

Protein phospholipase C Zeta1 expression in patients with failed ICSI but with normal sperm parameters

Lee

Arny

Grow

et al. 2014

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose This study was conducted to determine if expression of the testis-specific phospholipase C Zeta1 (PLCZ1) correlated with low success or fertilization failure after ICSI in patients with normal parameters after standard semen analysis (SA). Methods Couples <43 years with one or two failed or low fertilization ICSI cycles. Standard Semen Analysis (SA) was performed to determine sperm parameters in male partners, whereas females were evaluated for antral follicle counts (AFC), day 3 FSH levels and peak Estradiol (E2) levels. The presence of PLCZ1 in sperm was ascertained using Western blotting and Immunofluorescence (IF) analysis. The ability of sperm to initiate changes in the intracellular concentrations of free calcium ([Ca 2+ ] i ), which is characteristic of mammalian sperm, was performed after injection of human sperm into mouse eggs loaded with the Ca 2+ sensitive dye fura-2 AM. Results Male partners of couples with failed or low success ICSI fertilization but with normal SA parameters showed low expression levels of PLCZ1 as determined by western blotting and reduced fluorescent signal during IF studies. In addition, fewer of these males' sperm showed PLCZ1 expression and were able to initiate robust [Ca 2+ ] i oscillations upon injection into eggs. Conclusion Our data suggest that in patients with normal SA parameters but with repeated low fertilization or outright failed fertilization results after ICSI, abnormal PLCZ1 function should be considered as the underlying mechanism responsible for the failure of fertilization.

show abstract

“…1). Among the twelve oocytes examined, two (16.7%) showed pronuclear formation; however, the sperm aster was not observed (Terada et al 2009a). While not a universal finding, it is possible to identify those oocytes that fail to fertilize secondary to sperm aster dysplasia and sperm centrosomal dysfunction.…”

Section: Fertilization Failures After Icsimentioning

confidence: 88%

“…However, the occurrence of fertilization, itself, can be confirmed following IVF. Several interventions for IVF fertilization failure, including sperm manipulation, have been investigated (Yanagida et al 1999;Murase et al 2004;Tomashov-Matar et al 2005;Nasr-Esfahani et al 2008;Terada et al 2009a). However, most did not develop sufficiently well so as to gain acceptance into standard ART protocols.…”

Section: Post-icsi Events In Fertilization: Role Of the Sperm Centrosmentioning

confidence: 99%

Essential Roles of the Sperm Centrosome in Human Fertilization: Developing the Therapy for Fertilization Failure due to Sperm Centrosomal Dysfunction

Terada

Schatten

Hasegawa

et al. 2010

Tohoku J. Exp. Med.

Self Cite

View full text Add to dashboard Cite

Successful pregnancy after oocyte activation by a calcium ionophore for a patient with recurrent intracytoplasmic sperm injection failure, with an assessment of oocyte activation and sperm centrosomal function using bovine eggs

Cited by 40 publications

References 15 publications

An insufficient increase of cytosolic free calcium level results postovulatory aging-induced abortive spontaneous egg activation in rat

An insufficient increase of cytosolic free calcium level results postovulatory aging-induced abortive spontaneous egg activation in rat

Protein phospholipase C Zeta1 expression in patients with failed ICSI but with normal sperm parameters

Essential Roles of the Sperm Centrosome in Human Fertilization: Developing the Therapy for Fertilization Failure due to Sperm Centrosomal Dysfunction

Contact Info

Product

Resources

About