Successful Manipulation of the Gut Microbiome to Treat Spontaneous and Induced Murine Models of Colitis

Movva, Ramya; Murtaza, Nida; Giri, Rabina; Png, Chin Wen; Davies, Joan E.; Alabbas, Saleh; Oancea, Iulia; O'Cuiv, Páraic; Morrison, Mark; Begun, Jakob; Florin, Timothy H.

doi:10.1016/j.gastha.2021.12.008

Cited by 2 publications

(1 citation statement)

References 49 publications

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“…The manipulation of the gut microbiota (GM) through the addition of preoperative oral antibiotics and probiotics to reduce infectious complications in colorectal surgery may be the key to clinical success in AL and SSI prevention 14 . In mouse models, reproducible GM changes through different regimens of antibiotic cocktails have been frequently used 15,16 to deplete normal GM and mimic the sterile gut of germ-free mice, to explore the effects of GM in pathological conditions [17][18][19][20][21][22][23][24] , such as Clostridium di cile infection 18,19 , ulcerative colitis 23 , and colon tumorigenesis 24 .…”

Section: Introductionmentioning

confidence: 99%

Antibiotic cocktail followed by Clostridium butyricum (CBM588) supplementation improves colonic anastomotic healing in mice

Chen

Lai

et al. 2023

Preprint

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Background Microbiota manipulation through preoperative probiotic administration with Clostridium butyricum (CBM588) has shown promising results in reducing surgical site infections, but the resulting changes in gut microbiota are unclear. This study, using 16S ribosomal RNA metagenomic analysis, aimed to observe microbiome changes after CBM588 administration before murine colonic anastomosis. Materials and methods Eighteen C57BL/6 mice were divided into three groups. All mice, including group A as the control group, received oral neomycin and metronidazole before surgery. Group B received six consecutive days of CBM588 before surgery. Group C received kanamycin, gentamycin, colistin, metronidazole, and vancomycin, followed by six consecutive days of CBM588 before surgery. Results Compared with groups A and B, group C had significantly lower adhesion scores (p < 0.001), better postoperative performance (p < 0.05) and anastomotic healing scores (p < 0.001), and lower anastomotic necrosis areas (p < 0.05). Metagenomic analysis revealed that group C was enriched with the order Clostridiales, family Clostridiaceae, and genus Clostridium, with low bacterial diversity and composition. Conclusions Using a murine colonic anastomosis model, a preoperative antibiotic cocktail followed by CBM588 supplementation effectively decreased bacterial diversity and increased the percentage of Clostridiaceae. Associated outcomes included lower intra-abdominal adhesion scores, better postoperative performance and anastomotic healing scores, and lower anastomotic necrosis areas.

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