Substrate specificity of pullulanase

Purification and Some Properties of a Novel α-Amylase Produced by a Strain of Thermoactinomyces vulgaris

“…DEAE-cellulose (DE 32) and CMcellulose (CM-23) were purchased from Whatman Ltd. Isomaltose, maltotriose, pull ulan, and the pullulanase of Klebsiella aerogenes 3 ) were purchased from Hayashibara Biochemical Laboratories, Inc. Ampholine was purchased from LKB Produkter Co. Panose, isopanose, and the isopullulanase of Asp. niger 4 ) were kindly supplied by Dr. T. Kobayashi of Tokyo Noko University.…”

Sugarcane starch: quantitative determination and characterization

“…The pullulanase is an enzyme that hydrolyzes specifically the α-1,6 glycosidic bonds of starch and its by-products that contain at least two glucose units in the lateral branched chains (ABDULLAH;FRENCH, 1970). Whayman and Willesdorf (1976) reported that N:Co310 and Q58 sugarcane starch varieties presented 15% amylose, while potato starch contained 23% amylose.…”

Isoamylase and its Industrial Significance in the Production of Sugars from Starch

“….reversible, whereas that of pullulanase is reversible. Pullulanase polymerizes maltose~forming a tetrasaccharide, and links maltose with amylose (Abdullah and French, 1970); the yield of maltose is therefore much higher with isoamylase than with pullu w lanase.…”