Subrenal capsule assay for fresh human tumors in immunocompetent mice; an inappropriate technique for non-small cell lung cancer

Human Colon Adenocarcinoma in the SCID/CB6 Radiation Chimera Is Susceptible to Adoptive Transfer of Allogeneic Human Peripheral Blood Mononuclear Cells

“…hCEA was detected in situ and its level in mouse sera was correlated to tumor size and significantly decreased following tumor resection by means of nephrectomy or allogeneic human PBMC administration. Thus, while hCC has been previously established in mice (2,(8)(9)(10)(19)(20)(21)(22), our experimental design has several advantages over previous attempts. First, we have utilized direct transfer of surgical samples from patients rather than cell lines of colorectal carcinoma; second, engraftment of tumor fragments at the subcapsular space, enabled us to practice a complete tumor resection, by Mice were bled under anesthesia from the retro-orbital sinus 1 week before tumor resection by nephrectomy and subsequently were bled periodically.…”

“…Mice with internal tumors are generally killed at the end of an experimental protocol to determine how the tumors have responded. Only a single time point can be assessed through this approach, and multiple animals at each time point must be assessed to evaluate the kinetics of tumor growth and the effects of treatment (8)(9)(10). To overcome these problems, we sought to develop a model system of hCC that would recapitulate its clinical course in humans, and also define a serum marker that would provide a quantitative measure of neoplastic growth and an accurate measurement of the response of the tumor to a given treatment.…”

Human Colon Adenocarcinoma in the SCID/CB6 Radiation Chimera Is Susceptible to Adoptive Transfer of Allogeneic Human Peripheral Blood Mononuclear Cells

“…hCEA was detected in situ and its level in mouse sera was correlated to tumor size and significantly decreased following tumor resection by means of nephrectomy or allogeneic human PBMC administration. Thus, while hCC has been previously established in mice (2,(8)(9)(10)(19)(20)(21)(22), our experimental design has several advantages over previous attempts. First, we have utilized direct transfer of surgical samples from patients rather than cell lines of colorectal carcinoma; second, engraftment of tumor fragments at the subcapsular space, enabled us to practice a complete tumor resection, by Mice were bled under anesthesia from the retro-orbital sinus 1 week before tumor resection by nephrectomy and subsequently were bled periodically.…”

“…Mice with internal tumors are generally killed at the end of an experimental protocol to determine how the tumors have responded. Only a single time point can be assessed through this approach, and multiple animals at each time point must be assessed to evaluate the kinetics of tumor growth and the effects of treatment (8)(9)(10). To overcome these problems, we sought to develop a model system of hCC that would recapitulate its clinical course in humans, and also define a serum marker that would provide a quantitative measure of neoplastic growth and an accurate measurement of the response of the tumor to a given treatment.…”

Mouse Subrenal Capsule Assay Chemosensitivity and DNA Flow Cytometry Parameters of Human Melanoma Metastases

“…In studies on non-small-cell lung carcinomas (29) and on colorectal carcinomas (30) a high proportion of the tumor specimens contained no viable tumor cells after the 6-day assay despite the fact that they were optically measured to have grown in size under the renal capsule during the assay. In contrast, in the study by Dumont et al (31) all the melanoma specimens in the control group were confirmed to contain tumor cells at the end of the 6-day assay.…”

Tumour growth rate and DNA flow cytometry parameters as prognostic factors in metastatic melanoma

“…In studies on non-small cell lung carcinomas (Tueni et al, 1987) and on colorectal carcinoma (Kouri et al, 1989) a high proportion of the tumour specimens contained no viable tumour cells after the 6 day assay despite the fact that they were optically measured to have grown under the renal capsule during the assay. In contrast, in the study by Dumont and coworkers all the melanoma specimens in the control group were confirmed to contain tumour cells at the end of the 6-day assay (Dumont et al, 1984).…”

Human Colon Adenocarcinoma in the SCID/CB6 Radiation Chimera Is Susceptible to Adoptive Transfer of Allogeneic Human Peripheral Blood Mononuclear Cells

“…hCEA was detected in situ and its level in mouse sera was correlated to tumor size and significantly decreased following tumor resection by means of nephrectomy or allogeneic human PBMC administration. Thus, while hCC has been previously established in mice (2,(8)(9)(10)(19)(20)(21)(22), our experimental design has several advantages over previous attempts. First, we have utilized direct transfer of surgical samples from patients rather than cell lines of colorectal carcinoma; second, engraftment of tumor fragments at the subcapsular space, enabled us to practice a complete tumor resection, by Mice were bled under anesthesia from the retro-orbital sinus 1 week before tumor resection by nephrectomy and subsequently were bled periodically.…”

“…Mice with internal tumors are generally killed at the end of an experimental protocol to determine how the tumors have responded. Only a single time point can be assessed through this approach, and multiple animals at each time point must be assessed to evaluate the kinetics of tumor growth and the effects of treatment (8)(9)(10). To overcome these problems, we sought to develop a model system of hCC that would recapitulate its clinical course in humans, and also define a serum marker that would provide a quantitative measure of neoplastic growth and an accurate measurement of the response of the tumor to a given treatment.…”

Mouse Subrenal Capsule Assay Chemosensitivity and DNA Flow Cytometry Parameters of Human Melanoma Metastases

“…In studies on non-small-cell lung carcinomas (29) and on colorectal carcinomas (30) a high proportion of the tumor specimens contained no viable tumor cells after the 6-day assay despite the fact that they were optically measured to have grown in size under the renal capsule during the assay. In contrast, in the study by Dumont et al (31) all the melanoma specimens in the control group were confirmed to contain tumor cells at the end of the 6-day assay.…”

Tumour growth rate and DNA flow cytometry parameters as prognostic factors in metastatic melanoma

“…In studies on non-small cell lung carcinomas (Tueni et al, 1987) and on colorectal carcinoma (Kouri et al, 1989) a high proportion of the tumour specimens contained no viable tumour cells after the 6 day assay despite the fact that they were optically measured to have grown under the renal capsule during the assay. In contrast, in the study by Dumont and coworkers all the melanoma specimens in the control group were confirmed to contain tumour cells at the end of the 6-day assay (Dumont et al, 1984).…”

Human Colon Adenocarcinoma in the SCID/CB6 Radiation Chimera Is Susceptible to Adoptive Transfer of Allogeneic Human Peripheral Blood Mononuclear Cells

“…hCEA was detected in situ and its level in mouse sera was correlated to tumor size and significantly decreased following tumor resection by means of nephrectomy or allogeneic human PBMC administration. Thus, while hCC has been previously established in mice (2,(8)(9)(10)(19)(20)(21)(22), our experimental design has several advantages over previous attempts. First, we have utilized direct transfer of surgical samples from patients rather than cell lines of colorectal carcinoma; second, engraftment of tumor fragments at the subcapsular space, enabled us to practice a complete tumor resection, by Mice were bled under anesthesia from the retro-orbital sinus 1 week before tumor resection by nephrectomy and subsequently were bled periodically.…”

“…Mice with internal tumors are generally killed at the end of an experimental protocol to determine how the tumors have responded. Only a single time point can be assessed through this approach, and multiple animals at each time point must be assessed to evaluate the kinetics of tumor growth and the effects of treatment (8)(9)(10). To overcome these problems, we sought to develop a model system of hCC that would recapitulate its clinical course in humans, and also define a serum marker that would provide a quantitative measure of neoplastic growth and an accurate measurement of the response of the tumor to a given treatment.…”

Mouse Subrenal Capsule Assay Chemosensitivity and DNA Flow Cytometry Parameters of Human Melanoma Metastases

“…In studies on non-small-cell lung carcinomas (29) and on colorectal carcinomas (30) a high proportion of the tumor specimens contained no viable tumor cells after the 6-day assay despite the fact that they were optically measured to have grown in size under the renal capsule during the assay. In contrast, in the study by Dumont et al (31) all the melanoma specimens in the control group were confirmed to contain tumor cells at the end of the 6-day assay.…”

Tumour growth rate and DNA flow cytometry parameters as prognostic factors in metastatic melanoma

“…In studies on non-small cell lung carcinomas (Tueni et al, 1987) and on colorectal carcinoma (Kouri et al, 1989) a high proportion of the tumour specimens contained no viable tumour cells after the 6 day assay despite the fact that they were optically measured to have grown under the renal capsule during the assay. In contrast, in the study by Dumont and coworkers all the melanoma specimens in the control group were confirmed to contain tumour cells at the end of the 6-day assay (Dumont et al, 1984).…”