Sublethal and transgenerational effects of dinotefuran on biological parameters and behavioural traits of the mirid bug Apolygus lucorum

Abstract

The mirid bug, Apolygus lucorum, has become a major pest of many crops and fruit trees since the widespread adoption of Bt cotton in northern China. Neonicotinoid insecticides, such as dinotefuran, applied to control this pest may show sublethal effects, but evidence for such effects is lacking. Here, we investigated the sublethal and transgenerational effects of dinotefuran on biological parameters and feeding behavioural traits of A. lucorum using the age-stage, two-sex life table and electrical penetration graphs (EPGs), respectively. The LC 10 and Lc 30 of dinotefuran against 3 rd -instar nymphs of A. lucorum were 14.72 and 62.95 mg L −1 , respectively. These two concentrations significantly extended the development duration from 3 rd -instar nymph to adult in parent generation (F0). LC 30 also increased the oviposition period and male adult longevity and reduced nymphal survival rate in the F0. For offspring generation (F1), the egg duration, preadult duration, and total preoviposition period were significantly lower at LC 10 than in the control, and the egg duration, duration of 4 th -instar nymphs, preadult duration, oviposition period, and fecundity were also decreased at LC 30 . However, the four demographic parameters of F1 generation, namely, net reproductive rate (R 0 ), intrinsic rate of increase (r), finite rate of increase (λ), and mean generation time (T), were not affected by dinotefuran. The significant differences in the number of probes and duration of each of four feeding waveforms failed to be detected when A. lucorum nymphs treated by dinotefuran feed on Bt cotton plants without insecticide exposure. Overall, the dinotefuran concentrations tested here have sublethal, but no transgenerational impacts on A. lucorum.Mirid bugs, including Apolygus lucorum, have become the key pests of cotton and fruit trees owing to the major reduction in broad-spectrum insecticide use associated with the wide-scale adoption of Bt cotton in China since 1997 1,2 . The nymphs and adults of these bugs mainly feed on the young tissues of crops, including the leaves, bolls, fruits, terminal meristems, and other tissues, resulting in the stunting of plants and dropping of bolls and fruits, and in turn causing serious yield loss 3 . Broad-spectrum insecticides, such as organophosphates and pyrethroids, have been extensively applied to reduce the infestation by these insects 4-6 . However, many environmental risks are related to the use of these conventional insecticides, e.g., insect resistance 7,8 , with negative effects to natural enemies 9,10 and on human health. Thus, it is crucial that insecticides with highly effective to pests and low-toxicity to mammals should be adopted to manage mirid bugs to reduce the environmental risks.Neonicotinoid insecticides are among the most widely used pesticides globally, with the advantages of favourable toxicological properties, flexible use, and systemic activity 11,12 . They selectively act as agonists of insect nicotinic acetylcholine receptors (nAChRs), preventing signal tr...

Relationships Among the Feeding Behaviors of a Mirid Bug on Cotton Leaves of Different Ages and Plant Biochemical Substances

“…Similarly, EPG studies on mirid bugs do not have a universal naming convention. Earlier A. lucorum studies named waveform families (then termed phases) based on voltage patterns visible to the human eye (e.g., Zhao et al 2011b , Song et al 2017 , Lu et al 2020 ). However, some specific waveforms named therein were not well correlated with specific probing behaviors, so the name and definition of mirid waveforms are still evolving.…”

Lethal and sublethal effects of insecticides on Bathycoelia distincta (Heteroptera: Pentatomidae)

“…Sublethal concentrations of insecticides can affect many physiological parameters such as development, longevity, and fecundity (Zhou et al 2017;Lu et al 2020;Wu et al 2022), as well as mobility (da Silva et al 2022), and feeding behaviour (Koo et al 2015;Zeng et al 2016). These effects can vary according to the species and the insecticides used.…”

“…These effects can vary according to the species and the insecticides used. For example, sublethal concentrations of dinotefuran can reduce the fecundity of the planthopper Nilaparvata lugens (Stal) (Bao et al 2009) whereas no effect was observed on the mirid bug Apolygus lucorum (Meyer-Dur) (Lu et al 2020). In contrast, other studies have shown that sublethal concentrations of insecticides can stimulate pest population dynamics (e.g., insecticide-induced hormetic responses) by influencing their reproductive output.…”

Sublethal and transgenerational effects of a potential plant‐derived insecticide, β‐asarone, on population fitness of brown planthopper, Nilaparvata lugens

“…A significant increase in the transgenerational population growth of Rhopalosiphum padi L. was observed when these insects were sub-lethally exposed to LD 10 and LD 20 doses of dinotefuran (Deng et al, 2019). Exposure to the LC 30 of dinotefuran significantly extended the developmental duration from the third instar to adult Apolygus lucorum Meyer-Dür in the parental generation (F 0 ), but prolonged the oviposition period and male adult longevity as well as reduced the nymphal survival rate (Lu et al, 2020). Sublethal concentrations (LC 25 ) of sulfoxaflor can significantly increase the developmental duration in the first-generation progeny of Myzus persicae Sulzer and improve the reproductive capacity of adults (Tang et al, 2015).…”