Subchronic toxicity of 2,3,7,8-tetrabromodibenzo-p-dioxin in rats

The Influence of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) on Hematological Parameters During Experimentally Induced Pleuritis in Rats

“…According to other studies, application of TCDD dose seven times higher causes thymus, spleen, or lymphatic gland atrophy [51, 52]. Our studies also show the same effects of dioxin, despite using the seven times lower dose of TCDD [44, 45, 53]. Different TCDD doses are probably a cause of a distinct white blood cell picture in rats with experimentally induced pleuritis being under the effect of TCDD received in current and recent studies.…”

“…According to Vos et al [54, 55], low TCDD doses (about 0.2 μg per 1 kg of body weight) influence the humoral response displaying itself through the increase of α- and β-globulin levels and reduction of the ability for antibody synthesis in mice plasma. The dioxin’s affinity to DNA molecules suggests that dioxins could be responsible for the immunity decrease [45, 54, 55]. …”

The Influence of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) on Hematological Parameters During Experimentally Induced Pleuritis in Rats

“…According to other studies, application of TCDD dose seven times higher causes thymus, spleen, or lymphatic gland atrophy [51, 52]. Our studies also show the same effects of dioxin, despite using the seven times lower dose of TCDD [44, 45, 53]. Different TCDD doses are probably a cause of a distinct white blood cell picture in rats with experimentally induced pleuritis being under the effect of TCDD received in current and recent studies.…”

“…According to Vos et al [54, 55], low TCDD doses (about 0.2 μg per 1 kg of body weight) influence the humoral response displaying itself through the increase of α- and β-globulin levels and reduction of the ability for antibody synthesis in mice plasma. The dioxin’s affinity to DNA molecules suggests that dioxins could be responsible for the immunity decrease [45, 54, 55]. …”

Dose- And Time-Dependent Effects of 2,3,7,8-Tetrabromodibenzo-P-Dioxin on Rat Liver

“…Notably, the multinucleated hepatocytes occurred on Day 36 only in the female rats dosed 100 and 300 μg TBDD/kg, although Ivens et al (1992Ivens et al ( , 1993 did not state the TBDD-induced hepatocellular multinucleation. Our present finding is consistent with the pre- viously reported results showing that single and repeated oral administrations of TCDD to rats induced multinucleated hepatocytes (Jones and Butler, 1974;Kociba et al, 1976;Pohjanvirta et al, 1989;NTP, 2006).…”

“…According to Ivens et al (1992Ivens et al ( , 1993, acute hepatotoxicity induced by single administration of TBDD by gavage to male and female rats at dose levels of 10 to 300 μg/kg was histopathologically character- 4.52 ± 0.24 6.63 ± 0.48** 6.55 ± 0.46** 4.67 ± 0.28* 6.43 ± 0.51** 6.54 ± 0.50** 30 5.20 ± 0.24** 7.07 ± 0.80** 6.96 ± 0.55** 5.79 ± 0.69** 7.20 ± 0.52** 6.81 ± 0.62** 100 6.39 ± 0.10 a) 6.97 ± 0.41** 6.48 ± 0.74** 6.12 ± 0.26** 7.06 ± 0.19** 7.49 ± 1.16** 300 Total cholesterol (mg/dL) 69 ± 11 77 ± 5 68 ± 6 62 ± 9 82 ± 5 78 ± 10 0 93 ± 7* 129 ± 20** 82 ± 3 96 ± 12 109 ± 26 87 ± 6 10 110 ± 14** 115 ± 7** 81 ± 12 117 ± 15** 127 ± 16** 98 ± 9* 30 117 ± 21** 128 ± 18** 79 ± 8 139 ± 38** 126 ± 5** 107 ± 14** 100 120 ± 70 a) 163 ± 18** 82 ± 9 131 ± 26** 118 ± 21* 96 ± 12 300 Phospholipid (mg/dL) 163 ± 25 157 ± 10 143 ± 11 135 ± 15 175 ± 7 171 ± 19 0 201 ± 14* 231 ± 30** 170 ± 10 186 ± 8 199 ± 36 184 ± 24 10 218 ± 7** 211 ± 17* 169 ± 23 213 ± 23** 207 ± 22 200 ± 17 30 212 ± 29** 224 ± 30** 164 ± 15 231 ± 53** 212 ± 13 206 ± 11 100 226 ± 110 a) 292 ± 36** 160 ± 10 235 ± 47** 217 ± 35 201 ± 26 300 AST : Aspartate aminotransferase (IU/L) 48 ± 8 53 ± 5 54 ± 5 60 ± 7 63 ± 4 56 ± 2 0 60 ± 18 57 ± 5 62 ± 7 62 ± 7 59 ± 5 58 ± 5 10 62 ± 10 61 ± 8 * 62 ± 6 73 ± 17 57 ± 5 58 ± 8 30 97 ± 16** 78 ± 15 ** 64 ± 5 81 ± 21 76 ± 26 63 ± 4 100 186 ± 81 a) 311 ± 281 ** 61 ± 4 181 ± 195 81 ± 33 62 ± 4 300 Values are expresed as mean ± S.D. of 5 rats in each group without 300 μg/kg-dosed group at Day 36 in female rats.…”

Polybrominated Dibenzo-p-Dioxins, Dibenzofurans, and Biphenyls: Inclusion in the Toxicity Equivalency Factor Concept for Dioxin-Like Compounds

“…Toxicity studies have been reported for different brominated congeners using oral, ip, and sc administration (Ao et al, 2009;Haijima et al, 2010;Ivens et al, 1992Ivens et al, ,1993Mason et al, 1987a;Moore et al, 1979;Nagao et al, 1990;White et al, 2012). These studies indicate that the toxic effects in rodents for 2,3,7,8-TeBDD are in the same dose range as that of 2,3,7,8-TeCDD, indicating a comparable potency for both congeners.…”

The Influence of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) on Hematological Parameters During Experimentally Induced Pleuritis in Rats

“…According to other studies, application of TCDD dose seven times higher causes thymus, spleen, or lymphatic gland atrophy [51, 52]. Our studies also show the same effects of dioxin, despite using the seven times lower dose of TCDD [44, 45, 53]. Different TCDD doses are probably a cause of a distinct white blood cell picture in rats with experimentally induced pleuritis being under the effect of TCDD received in current and recent studies.…”

“…According to Vos et al [54, 55], low TCDD doses (about 0.2 μg per 1 kg of body weight) influence the humoral response displaying itself through the increase of α- and β-globulin levels and reduction of the ability for antibody synthesis in mice plasma. The dioxin’s affinity to DNA molecules suggests that dioxins could be responsible for the immunity decrease [45, 54, 55]. …”

Dose- And Time-Dependent Effects of 2,3,7,8-Tetrabromodibenzo-P-Dioxin on Rat Liver

“…Notably, the multinucleated hepatocytes occurred on Day 36 only in the female rats dosed 100 and 300 μg TBDD/kg, although Ivens et al (1992Ivens et al ( , 1993 did not state the TBDD-induced hepatocellular multinucleation. Our present finding is consistent with the pre- viously reported results showing that single and repeated oral administrations of TCDD to rats induced multinucleated hepatocytes (Jones and Butler, 1974;Kociba et al, 1976;Pohjanvirta et al, 1989;NTP, 2006).…”

“…According to Ivens et al (1992Ivens et al ( , 1993, acute hepatotoxicity induced by single administration of TBDD by gavage to male and female rats at dose levels of 10 to 300 μg/kg was histopathologically character- 4.52 ± 0.24 6.63 ± 0.48** 6.55 ± 0.46** 4.67 ± 0.28* 6.43 ± 0.51** 6.54 ± 0.50** 30 5.20 ± 0.24** 7.07 ± 0.80** 6.96 ± 0.55** 5.79 ± 0.69** 7.20 ± 0.52** 6.81 ± 0.62** 100 6.39 ± 0.10 a) 6.97 ± 0.41** 6.48 ± 0.74** 6.12 ± 0.26** 7.06 ± 0.19** 7.49 ± 1.16** 300 Total cholesterol (mg/dL) 69 ± 11 77 ± 5 68 ± 6 62 ± 9 82 ± 5 78 ± 10 0 93 ± 7* 129 ± 20** 82 ± 3 96 ± 12 109 ± 26 87 ± 6 10 110 ± 14** 115 ± 7** 81 ± 12 117 ± 15** 127 ± 16** 98 ± 9* 30 117 ± 21** 128 ± 18** 79 ± 8 139 ± 38** 126 ± 5** 107 ± 14** 100 120 ± 70 a) 163 ± 18** 82 ± 9 131 ± 26** 118 ± 21* 96 ± 12 300 Phospholipid (mg/dL) 163 ± 25 157 ± 10 143 ± 11 135 ± 15 175 ± 7 171 ± 19 0 201 ± 14* 231 ± 30** 170 ± 10 186 ± 8 199 ± 36 184 ± 24 10 218 ± 7** 211 ± 17* 169 ± 23 213 ± 23** 207 ± 22 200 ± 17 30 212 ± 29** 224 ± 30** 164 ± 15 231 ± 53** 212 ± 13 206 ± 11 100 226 ± 110 a) 292 ± 36** 160 ± 10 235 ± 47** 217 ± 35 201 ± 26 300 AST : Aspartate aminotransferase (IU/L) 48 ± 8 53 ± 5 54 ± 5 60 ± 7 63 ± 4 56 ± 2 0 60 ± 18 57 ± 5 62 ± 7 62 ± 7 59 ± 5 58 ± 5 10 62 ± 10 61 ± 8 * 62 ± 6 73 ± 17 57 ± 5 58 ± 8 30 97 ± 16** 78 ± 15 ** 64 ± 5 81 ± 21 76 ± 26 63 ± 4 100 186 ± 81 a) 311 ± 281 ** 61 ± 4 181 ± 195 81 ± 33 62 ± 4 300 Values are expresed as mean ± S.D. of 5 rats in each group without 300 μg/kg-dosed group at Day 36 in female rats.…”

Polybrominated Dibenzo-p-Dioxins, Dibenzofurans, and Biphenyls: Inclusion in the Toxicity Equivalency Factor Concept for Dioxin-Like Compounds

“…Toxicity studies have been reported for different brominated congeners using oral, ip, and sc administration (Ao et al, 2009;Haijima et al, 2010;Ivens et al, 1992Ivens et al, ,1993Mason et al, 1987a;Moore et al, 1979;Nagao et al, 1990;White et al, 2012). These studies indicate that the toxic effects in rodents for 2,3,7,8-TeBDD are in the same dose range as that of 2,3,7,8-TeCDD, indicating a comparable potency for both congeners.…”

The Influence of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) on Hematological Parameters During Experimentally Induced Pleuritis in Rats

“…According to other studies, application of TCDD dose seven times higher causes thymus, spleen, or lymphatic gland atrophy [51, 52]. Our studies also show the same effects of dioxin, despite using the seven times lower dose of TCDD [44, 45, 53]. Different TCDD doses are probably a cause of a distinct white blood cell picture in rats with experimentally induced pleuritis being under the effect of TCDD received in current and recent studies.…”

“…According to Vos et al [54, 55], low TCDD doses (about 0.2 μg per 1 kg of body weight) influence the humoral response displaying itself through the increase of α- and β-globulin levels and reduction of the ability for antibody synthesis in mice plasma. The dioxin’s affinity to DNA molecules suggests that dioxins could be responsible for the immunity decrease [45, 54, 55]. …”

Dose- And Time-Dependent Effects of 2,3,7,8-Tetrabromodibenzo-P-Dioxin on Rat Liver

“…Notably, the multinucleated hepatocytes occurred on Day 36 only in the female rats dosed 100 and 300 μg TBDD/kg, although Ivens et al (1992Ivens et al ( , 1993 did not state the TBDD-induced hepatocellular multinucleation. Our present finding is consistent with the pre- viously reported results showing that single and repeated oral administrations of TCDD to rats induced multinucleated hepatocytes (Jones and Butler, 1974;Kociba et al, 1976;Pohjanvirta et al, 1989;NTP, 2006).…”

“…According to Ivens et al (1992Ivens et al ( , 1993, acute hepatotoxicity induced by single administration of TBDD by gavage to male and female rats at dose levels of 10 to 300 μg/kg was histopathologically character- 4.52 ± 0.24 6.63 ± 0.48** 6.55 ± 0.46** 4.67 ± 0.28* 6.43 ± 0.51** 6.54 ± 0.50** 30 5.20 ± 0.24** 7.07 ± 0.80** 6.96 ± 0.55** 5.79 ± 0.69** 7.20 ± 0.52** 6.81 ± 0.62** 100 6.39 ± 0.10 a) 6.97 ± 0.41** 6.48 ± 0.74** 6.12 ± 0.26** 7.06 ± 0.19** 7.49 ± 1.16** 300 Total cholesterol (mg/dL) 69 ± 11 77 ± 5 68 ± 6 62 ± 9 82 ± 5 78 ± 10 0 93 ± 7* 129 ± 20** 82 ± 3 96 ± 12 109 ± 26 87 ± 6 10 110 ± 14** 115 ± 7** 81 ± 12 117 ± 15** 127 ± 16** 98 ± 9* 30 117 ± 21** 128 ± 18** 79 ± 8 139 ± 38** 126 ± 5** 107 ± 14** 100 120 ± 70 a) 163 ± 18** 82 ± 9 131 ± 26** 118 ± 21* 96 ± 12 300 Phospholipid (mg/dL) 163 ± 25 157 ± 10 143 ± 11 135 ± 15 175 ± 7 171 ± 19 0 201 ± 14* 231 ± 30** 170 ± 10 186 ± 8 199 ± 36 184 ± 24 10 218 ± 7** 211 ± 17* 169 ± 23 213 ± 23** 207 ± 22 200 ± 17 30 212 ± 29** 224 ± 30** 164 ± 15 231 ± 53** 212 ± 13 206 ± 11 100 226 ± 110 a) 292 ± 36** 160 ± 10 235 ± 47** 217 ± 35 201 ± 26 300 AST : Aspartate aminotransferase (IU/L) 48 ± 8 53 ± 5 54 ± 5 60 ± 7 63 ± 4 56 ± 2 0 60 ± 18 57 ± 5 62 ± 7 62 ± 7 59 ± 5 58 ± 5 10 62 ± 10 61 ± 8 * 62 ± 6 73 ± 17 57 ± 5 58 ± 8 30 97 ± 16** 78 ± 15 ** 64 ± 5 81 ± 21 76 ± 26 63 ± 4 100 186 ± 81 a) 311 ± 281 ** 61 ± 4 181 ± 195 81 ± 33 62 ± 4 300 Values are expresed as mean ± S.D. of 5 rats in each group without 300 μg/kg-dosed group at Day 36 in female rats.…”

Polybrominated Dibenzo-p-Dioxins, Dibenzofurans, and Biphenyls: Inclusion in the Toxicity Equivalency Factor Concept for Dioxin-Like Compounds

“…Toxicity studies have been reported for different brominated congeners using oral, ip, and sc administration (Ao et al, 2009;Haijima et al, 2010;Ivens et al, 1992Ivens et al, ,1993Mason et al, 1987a;Moore et al, 1979;Nagao et al, 1990;White et al, 2012). These studies indicate that the toxic effects in rodents for 2,3,7,8-TeBDD are in the same dose range as that of 2,3,7,8-TeCDD, indicating a comparable potency for both congeners.…”