Subcellular localization of leucine aminotransferase and α-hydroxyacid dehydrogenase in Trypanosoma cruzi

Trypanosoma cruzi: inhibition of alpha-hydroxyacid dehydrogenase isozyme II by N-allyl and N-propyl oxamates and their effects on intact epimastigotes

“…We also determined IC 50 values, and there were 4.25 mM, 0.33 mM and 0.32 mM, for oxamate, NAOx and NPOx, respectively. The K m value (0.3 mM) was similar to those reported by others (Montamat et al 1987). When we used T. cruzi homogenates containing HADH-isozyme II and HADH- isozyme I, the inhibitory effects of oxamate derivatives were the same as those presented for the purified isozymes, using α-ketoisocaproate as substrate for isozyme II and pyruvate for isozyme I (results not shown).…”

“…In this investigation we designed, synthesized, and tested NPOx and NAOx, as well as the ethyl ester of these oxamates on the activity of T. cruzi HADH-isozyme II, because this isozyme has been associated with metabolic pathways supplying energy for the motility and survival of this parasite (Coronel et al 1981, Montamat et al 1987.…”

“…It is interesting that HADH-isozyme II found in T. cruzi, shows a substrate spectrum similar to that of LDH-C4 (Coronel et al 1981, Montamat et al 1988, suggesting that this HADH- isozyme II may also accomplish very specific functions in T. cruzi (Montamat et al 1987). On account of the similarities between HADH-isozyme II and LDH-C4, it has been proposed that these isozymes are functionally homologous (Coronel et al 1981, Montamat et al 1987, 1988 and they must be integrated in metabolic pathways supplying energy for the motility of flagellum and survival of the parasite (Coronel et al 1981, Montamat et al 1987 or the spermatozoa, respectively (Coronel et al 1986). HADHisozyme II is also located in the cytosol and in the matrix of mitochondria (Montamat et al 1987).…”

“…On account of the similarities between HADH-isozyme II and LDH-C4, it has been proposed that these isozymes are functionally homologous (Coronel et al 1981, Montamat et al 1987, 1988 and they must be integrated in metabolic pathways supplying energy for the motility of flagellum and survival of the parasite (Coronel et al 1981, Montamat et al 1987 or the spermatozoa, respectively (Coronel et al 1986). HADHisozyme II is also located in the cytosol and in the matrix of mitochondria (Montamat et al 1987). On the basis of this dual subcellular distribution, it was proposed that this dehydrogenase, could be also integrated in a shuttle system, transferring reducing equivalents from cytosol to mitochondria, using branched-chain 2-hydroxyacids/ 2-oxo acids as the redox couple (Coronel et al 1986, Montamat et al 1987.…”

“…HADHisozyme II is also located in the cytosol and in the matrix of mitochondria (Montamat et al 1987). On the basis of this dual subcellular distribution, it was proposed that this dehydrogenase, could be also integrated in a shuttle system, transferring reducing equivalents from cytosol to mitochondria, using branched-chain 2-hydroxyacids/ 2-oxo acids as the redox couple (Coronel et al 1986, Montamat et al 1987.…”