Subacute cadmium exposure disrupts the hypothalamic-pituitary-gonadal axis, leading to polycystic ovarian syndrome and premature ovarian failure features in female rats

Costa, Charles S. da; Oliveira, Thiago Fernandes de; Freitas-Lima, Leandro Ceotto; Padilha, Alessandra Simão; Krause, Maiara; Carneiro, Maria Tereza Weitzel Dias; Salgado, Breno S.; Graceli, Jones Bernardes

doi:10.1016/j.envpol.2020.116154

Cited by 48 publications

(28 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Animals exhibited irregular estrous cycles following a single moderate dose of X-ray radiation, which further confirmed damage to the female reproductive system caused by ionizing radiation. This is consistent with previously reported ovarian dysfunction symptoms of menstrual irregularity ( 34 ). Animals in the FA pre-administration group had more regular menstrual cycles, suggestive of a protective effect of FA on ovarian function.…”

Section: Discussionsupporting

confidence: 94%

Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice

et al. 2022

View full text Add to dashboard Cite

Radiological therapy/examination is the primary source of artificial radiation exposure in humans. While its application has contributed to major advances in disease diagnosis and treatment, ionizing radiation exposure is associated with ovarian damage. The use of natural products, either alone or as an adjunct, has become increasingly common for reducing the side effects of radiological therapy during disease treatment. Herein, we explored the protective effect of folic acid (FA), a widely used B vitamin, against radiation-induced ovarian injury and its mechanism of action. Female mice with normal ovarian function were randomly divided into control, FA, radiation, and radiation + FA groups. The intervention strategy included daily intragastric administration of FA (5 mg/kg) for 3 weeks prior to radiation exposure. Mice in the radiation and radiation + FA groups received a single dose of 5 Gy X-ray irradiation. Changes in the estrous cycle were then recorded, and ovarian tissues were collected. Pathophysiological changes as well as reproductive and endocrine-related indexes were determined via H&E staining, immunohistochemistry, Western blot, and ELISA. The reproductive performance and emotional symptoms of animals were also monitored. Our results indicated that FA intervention effectively alleviated ovarian damage, leading to more regular estrous cycles, lesser impairment of follicular morphology and endocrine status, as well as greater germ cell preservation. Reduced levels of oxidative stress, inflammation, and enhanced DNA repair were associated these changes. FA pre-administration improved the reproductive performance, leading to higher pregnancy rates and greater litter sizes. Further, the anxiety levels of animals were significantly reduced. Our results indicate that FA pre-administration significantly alleviates radiation-induced ovarian damage in rodents, highlighting its potential as a protective strategy against radiation exposure in the female population.

show abstract

Section: Discussionsupporting

confidence: 94%

Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Cadmium (Cd) can cause impaired expression of hypothalamic genes, high concentrations of LH, and low concentrations of AMH in plasma, and abnormal follicular growth with associated reduction of antral follicles. The main results of these effects are alteration of the estrus cycle, premature ovary failure (POF), and polycystic ovary syndrome (PCOS) [ 165 , 166 ].…”

Section: Ovariesmentioning

confidence: 99%

Impact of Chemical Endocrine Disruptors and Hormone Modulators on the Endocrine System

Guarnotta

Amodei

Frasca

et al. 2022

IJMS

View full text Add to dashboard Cite

There is growing concern regarding the health and safety issues of endocrine-disrupting chemicals (EDCs). Long-term exposure to EDCs has alarming adverse health effects through both hormone-direct and hormone-indirect pathways. Non-chemical agents, including physical agents such as artificial light, radiation, temperature, and stress exposure, are currently poorly investigated, even though they can seriously affect the endocrine system, by modulation of hormonal action. Several mechanisms have been suggested to explain the interference of EDCs with hormonal activity. However, difficulty in quantifying the exposure, low standardization of studies, and the presence of confounding factors do not allow the establishment of a causal relationship between endocrine disorders and exposure to specific toxic agents. In this review, we focus on recent findings on the effects of EDCs and hormone system modulators on the endocrine system, including the thyroid, parathyroid glands, adrenal steroidogenesis, beta-cell function, and male and female reproductive function.

show abstract

“…12 Da Costa et al, also conducted an experimental research on cadmium exposures by oral routes but they did not found any significant difference in the thickness of endometrium, however they found decrease of myometrium thickness in the experimental group. 6 Nasiadek et al, also conduct a study with oral cadmium and found that cadmium in small doses tend to express estrogenic effect and in higher doses cadmium tend to express antiestrogenic effect. They also found that lower dose cadmium significantly increased the thickness of endometrium but in higher dose cadmium they found noticeable endometrial atrophy, therefore it concluded that cadmium can have both anti-estrogenic and estrogenic effect.…”

Section: Endometrial Thicknessmentioning

confidence: 99%

“…da Costa et al, found reduced number of corpus luteum in experimental group. 6 Sadeu and Foster also did a research with cigarette smoke condensate (CSC) and found that estradiol output significantly decrease after CSC exposure, this could lead to the disruption on the follicular development. 24 This research does not measure the rats steroid hormones level, even so it might have influence the results in this study.…”

Section: Number Of Ovarian Folliclesmentioning

confidence: 99%

“…5 In previous study cadmium exposures also cause reduced number of corpus luteum, primary follicles and anthral follicles. 6 Other findings show that N-nitroso-amine that is contain in cigarrate smoke can cause follicle destruction. Liu et al, found that N-nitroso amine, one of the component found in cigarette, exposure can reduce the number of primordial and primary follicle.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Effect of Acute Exposure of Cigarette Smoke on Endometrium Thickness and Number of Ovarian Follicles in Rats

Putri

Aswin

Yuliawati

2021

IABJ

View full text Add to dashboard Cite

Background: Cigarrete smoke has been ascociated with many health-related problems especially in long time use. One of many health problems that are affected is the reproductive system that give negative impact in fertility. However, there are limited studies about this in acute exposure of cigarette smoke. Objective: The aim of this study is to investigate the effect of acute exposure of cigarette smoke on endometrial thickness and number of ovarian follicles in rats. Methods: Thirty adult female rats were randomly divided into 3 groups: P0, P1 and P2. The P1 and P2 groups were exposed to cigarette smoke for 15 days with dose of 2 and 4 cigarettes daily, respectively. Vaginal swab was done twice, before and after the last treatment, to determine the proestrus phase of the animals. Histological slide of uterus and ovarium using Haematoxylin Eosin staining were observed under light microscop. Number of ovarian follicles were measured, and the thickness of endometrium was meassured using CellSens software version 2.3 (Olympus Life Science). Statistical analysis of data was performed using MANOVA in SPSS software version 23 for Windows. Results: There were no significant differences in both endometrial thickness and number of ovarian follicles between 3 groups (p>0.05). However, we found that there were a downward trend of some variables (endometrial thickness, number of primary follicles, de Graff follicles, and corpus luteum) from P0 to P2. Conclusion: We concluded that acute exposure of cigarette smoke not yet influenced the female reproductive organ, based on histological appearance of endemotrium and ovarium.

show abstract

Subacute cadmium exposure disrupts the hypothalamic-pituitary-gonadal axis, leading to polycystic ovarian syndrome and premature ovarian failure features in female rats

Cited by 48 publications

References 67 publications

Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice

Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice

Impact of Chemical Endocrine Disruptors and Hormone Modulators on the Endocrine System

Effect of Acute Exposure of Cigarette Smoke on Endometrium Thickness and Number of Ovarian Follicles in Rats

Contact Info

Product

Resources

About