Style-specific and developmentally regulated accumulation of a glycosylated thaumatin/PR5-like protein in Japanese pear ( Pyrus serotina Rehd.)

Sassa, Hidenori; Hirano, Hisashi

doi:10.1007/s004250050350

Cited by 50 publications

(33 citation statements)

References 45 publications

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“…Some fruit-and¯ower-related TLPs (Prunus-TLP from Prunus avium, ARATH-TLP from Arabidopsis thaliana, Brassica-TLP from Brassica campestris and PsTL1, the stylar PR5-like protein from Japanese pear) are clustered into a third branch together with other TLPs such as the TLP-kinase receptor from A. thaliana (PR5K). A very similar classi®cation of TLP was previously reported by Sassa and Hirano (1998).…”

Section: Relationships Of Ban-tlp With Other Tlpsmentioning

confidence: 58%

Purification and structural analysis of an abundant thaumatin-like protein from ripe banana fruit

Barre

Peumans

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et al. 2000

Planta

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The pulp of ripe bananas (Musa acuminata) contains an abundant thaumatin-like protein (TLP). Characterization of the protein and molecular cloning of the corresponding gene from banana demonstrated that the native protein consists of a single polypeptide chain of 200 amino acid residues. Molecular modelling further revealed that the banana thaumatin-like protein (Ban-TLP) adopts an overall fold similar to that of thaumatin and thaumatin-like PR-5 proteins. Although the banana protein exhibits an electrostatically polarized surface, which is believed to be essential for the antifungal properties of TLPs, it is apparently devoid of antifungal activity towards pathogenic fungi. It exhibits a low but detectable in vitro endo-beta-1,3-glucanase (EC 3.2.1.x) activity. As well as being present in fruits, Ban-TLP also occurs in root tips where its accumulation is enhanced by methyl jasmonate treatment of plants. Pulp of plantains (Musa acuminata) also contains a very similar TLP, which is even more abundant than its banana homologue. Our results demonstrate for the first time that fruit-specific (abundant) TLPs are not confined to dicots but occur also in fruits of monocot species. The possible role of the apparent widespread accumulation of fruit-specific TLPs is discussed.

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Section: Relationships Of Ban-tlp With Other Tlpsmentioning

confidence: 58%

Purification and structural analysis of an abundant thaumatin-like protein from ripe banana fruit

Barre

Peumans

Menu-Bouaouiche

et al. 2000

Planta

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“…Total RNA was extracted from the pistils of P. inflata (S 3L S 3L ), and reverse transcribed with a d(T) 17 adapter primer to generate cDNA as described in Sassa and Hirano (1998) and Ushijima et al (2003). A PCR primer (FHT1, 5¢-GTTCTTTTGATAATATCATCAG-3¢) was designed on the basis of an alignment of reported HT sequences.…”

Section: Cloning Of Cdna and Genomic Fragmentmentioning

confidence: 99%

Identification of a new class of pistil-specific proteins of Petunia inflata that is structurally similar to, but functionally distinct from, the self-incompatibility factor HT

Sassa

Hirano

2005

Mol Genet Genomics

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Pollen-pistil interactions are thought to involve a wide variety of intercellular recognition events controlled by diverse proteins and other molecules. One of the best characterized interactions is the S-RNase-based gametophytic self-incompatibility (GSI) system found in Solanaceae, Rosaceae and Scrophulariaceae. Although the S specificity of the pistil and the pollen in these families is determined by the S locus-encoded proteins S-RNase and SLF/SFB, respectively, these proteins alone are not sufficient for operation of the GSI reaction. Other factors are also required and are classified into three groups. To date, the only known factor is the pistil-expressed small asparagine-rich protein HT-B in three solanaceous genera Nicotiana, Lycopersicon and Solanum. HT-B is a Group 2 factor that is required for pollen rejection but do not affect S-RNase expression; factors in the other groups have not yet cloned. Here, we identified a new class of HT-like proteins in the style of Petunia inflata and named it HTL. Through alternative splicing, it was found that two isolated homologous HTL cDNAs, HTL-A and HTL-B, derived from a single gene. Like HT-B, HTL showed pistil-specific accumulation as well as significant sequence similarity to HT including conserved cystein residues at the C-terminal region and a signal peptide for extracellular localization. However, unlike HT-B, HTL lacked an asparagine-rich domain. Thus, it represents a new class of HT proteins. To determine whether HTL is involved in GSI function, RNA silencing constructs for HTL-A and HTL-B were introduced into self-incompatible P. inflata. Although several transgenic lines showed no detectable levels of both HTL-A and HTL-B transcripts, they retained normal GSI function and produced large fruits upon compatible pollination. This suggests that since silencing of the HTL gene alone is not sufficient to affect reproductive physiology, the gene is functionally distinct from the GSI factor HT-B.

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“…Thaumatin-like (TL) proteins have also been found in other plant organs, such as leaves and flowers (Uknes et al, 1992;Malehorn et al, 1994;Sassa and Hirano, 1998), but they are detected mainly in ripening fruit (Fils-Lycaon et al, 1996;Clendennen and May, 1997;Tattersall et al, 1997;Sassa and Hirano, 1998;Kim et al, 2002;Wang and Ng, 2002). In the cherr~ TL proteins comprise up to 42% of the total soluble proteins in the mesocarp at the ripe stage (Fils-Lycaon et al, 1996).…”

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confidence: 97%

Molecular characterization of a fruit-preferential thaumatin-like gene from apple (Malus domestica cv. Fuji)

2003

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Functioning of the thaumatin-like (TL) protein is known to be pathogenesis-or stress-related. Here we identified TL protein cDNA, from an apple-skin library, that was nearly identical to that of the MdTL1 gene. Transcripts of this so-named gene, MdTLla, were highly expressed in the fruit, but rarely in other tissue types. Expression was found in both the skin and the flesh of the fruit. We also examined the environmental or hormonal control of MdTLla expression. Exposing the fruit to light caused this gene to be induced in the skin tissues. Accumulation of IHdTLla mRNA reached a peak between Days I and 5 after exposure. In the leaves, MdTLIa was induced by salicylic acid (SA), but was not significantly affected by any other stresses. Analysis of the IHdTLla genomic clone, 2~TL1, revealed that transcription of MdTLla begins 53 bp upstream of the start codon. Sequence analysis of the ca. 1.0-kb MdTLla promoter region has enabled us to predict that it has a stress-related cis-element, such as the ABA responsive element (ABRE), as well as a light-responsive GT-1 and I-box.

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Style-specific and developmentally regulated accumulation of a glycosylated thaumatin/PR5-like protein in Japanese pear ( Pyrus serotina Rehd.)

Cited by 50 publications

References 45 publications

Purification and structural analysis of an abundant thaumatin-like protein from ripe banana fruit

Purification and structural analysis of an abundant thaumatin-like protein from ripe banana fruit

Identification of a new class of pistil-specific proteins of Petunia inflata that is structurally similar to, but functionally distinct from, the self-incompatibility factor HT

Molecular characterization of a fruit-preferential thaumatin-like gene from apple (Malus domestica cv. Fuji)

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