Studying the cytolytic activity of gas plasma with self-signalling phospholipid vesicles dispersed within a gelatin matrix

Self Cite

Section: Resultsmentioning

confidence: 94%

The assessment of cold atmospheric plasma treatment of DNA in synthetic models of tissue fluid, tissue and cells

Szili

Gaur

Hong

et al. 2017

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“…This is mainly due to the liquidity of water and the irregular activity of aqueous molecules which lead to a big challenge for spatially resolved measurements [12,13]. Recently, gelatin gel has been used by Szili et al as a surrogate for real tissue to study the penetration of RONS generated by a plasma jet [14][15][16]. The gelatin gel consists of water and collagen, the most abundant extracellular matrix protein, and the collagen inhibits the liquidity of water.…”

Section: Introductionmentioning

confidence: 99%

A ‘tissue model’ to study the barrier effects of living tissues on the reactive species generated by surface air discharge

He¹,

Liu²,

Xu³

et al. 2016

“…In order to address this issue, we developed a 'tissue model' to study the plasma interactions with phospholipid vesicles (mimics of cell membranes) encapsulated within a gelatin matrix (surrogate of tissue) [23]. We discovered that plasma can rupture vesicles > 150 m below the gelatin surface.…”

mentioning

confidence: 99%

Ionized gas (plasma) delivery of reactive oxygen species (ROS) into artificial cells

Hong¹,

Szili²,

Jenkins³

et al. 2014

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This study was designed to enhance our understanding of how reactive oxygen species (ROS), generated ex-situ by ionized gas (plasma), can affect the regulation of signalling processes within cells. A model system, comprising of a suspension of phospholipid vesicles (cell mimics) encapsulating a ROS reporter, was developed to study the plasma delivery of ROS into cells. For the first time it was shown that plasma unequivocally delivers ROS into cells over a sustained period and without compromising cell membrane integrity. An important consideration in cell and biological assays is the presence of serum, which significantly reduced the transfer efficiency of ROS into the vesicles. These results are key to understanding how plasma treatments can be tailored for specific medical or biotechnology applications. Further, the phospholipid vesicle ROS reporter system may find use in other studies involving the application of free radicals in biology and medicine.