Studying endothelial cell shedding and orientation using adaptive perfusion‐culture in a microfluidic vascular chip

Zhang, Xiang; Wang, Zhenxing; Zhang, Yu Shrike; Yan, Shujie; Hou, Chuanyu; Gong, Youping; Qiu, Jingjiang; Chen, Mo; Li, Qian

doi:10.1002/bit.27626

Cited by 4 publications

(5 citation statements)

References 42 publications

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“…One vessel chip incorporated electrospun fibrous scaffolds that enhanced the flow‐induced alignment of ECs. [ 228 ] Three strategies were employed in the vessel chip to change the flow rate from static conditions to artery flow‐related ones, including gradual, immediate, and intermittent schemes. The gradual increase in flow rate was better than the other two for reducing the shedding of ECs.…”

Section: Vascular Physiology and On‐chip Recapitulationmentioning

confidence: 99%

“…[ 217 ] This strategy of the gradually increased flow rate can be useful for screening culture parameters and investigating vascular biology. [ 228 ]…”

Section: Vascular Physiology and On‐chip Recapitulationmentioning

confidence: 99%

“…[219][220][221] Blood vessel chips have been employed to investigating the impact of τ w on vascular morphology and functions. [222] Compared with conventional approaches, including cone and plate systems [223] and parallel flow chamber, [224] blood vessel chips are advantageous in incorporating an array of biomimetic microenvironments, [222] including complex and tissuespecific flow profiles, [225,226] 3D culturing matrices, [198,227] and structured scaffold materials, [228] which support long-term cell culture and are associated with cellular response to the shear stress.…”

Section: Shear Stressmentioning

confidence: 99%

“…[217] This strategy of the gradually increased flow rate can be useful for screening culture parameters and investigating vascular biology. [228] Another vessel chip allows the well-controlled manipulation of a disturbed, oscillatory flow profile for studying YAP mechanobiology. [180] YAP is sensitive to both matrix stiffness [229] and shear stress, [230] and has important implications for atherosclerotic progression.…”

Section: Shear Stressmentioning

confidence: 99%

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Building Blood Vessel Chips with Enhanced Physiological Relevance

Gerhard‐Herman

Zhang

2023

Adv Materials Technologies

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Due to the bioengineering nature, tissue chips allow the manipulation of cellular composition [11] and a range of biophysical and biochemical environmental cues, including but not limited to cell-ECM interactions, dynamic flows, chemical species gradients, substrate stiffness, [12] and active mechanical stimuli. [13,14] In particular, the on-chip adoption of patients' cells, either primary or induced human pluripotent stem cells (hiPSCs), makes a bespoke and patient-specific tissue model in line with the goal of precision medicine. [15] In contrast, small animal-based models exhibit notable genetic and cellular differences from human physiology, which may lead to biased results regarding disease pathogenesis and therapeutic effects. [16,17] Furthermore, tissue chips can precisely control and decouple multiple experimental factors, beneficial for exploiting the inherently complex human physiology, in contrast to other in vitro models, such as Petri dish-based ones, which are focused on maintaining cell proliferation in vitro. Thus, tissue chips, as a viable alternative to other tissue/disease-modeling approaches, are promising for in vitro recapitulation of the sophisticated human physiology and pathology at the tissue and organ level and are expected to transform the landscapes of fundamental biological research, [18][19][20] drug screening and toxicology, [21][22][23][24] and possibly, clinical trials. [25,26] Tissue chips can construct a broad range of tissue-and organ-analogs in vitro, including the vessel and vascular networks, [27][28][29] muscles, [30][31][32][33] liver, [34][35][36] lung, [37][38][39] brain, [40,41] kidney, [42][43][44][45][46] gut, [47,48] bone marrow, [49] corneal, [50,51] tumor, [52][53][54][55][56] as well as the integrated multiple tissues/organs, [57][58][59] such as liver-kidney, [60][61][62] neuromuscular junction, [63] and a recirculating system with up to 13 organs. [64] Of note, these tissue chips may rely on the same set of technical approaches yet recapitulate the specific tissue (patho)physiology by considering tissue-specific cellular and matrix composition.Among all tissues/organs modeled in the chips, the blood vessel is of particular interest, largely for the following reasons (Figure 1). [65,66] i) The blood vessel exists in all tissues/ organs throughout the body up to the epithelial layer covering surfaces. Thus, the on-chip reconstruction of the vessel or its analog is often a prerequisite for constructing a tissue analog.ii) The blood vessel is both the structural basis of circulating blood flow throughout the body and responsible for many Blood vessel chips are bioengineered microdevices, consisting of biomaterials, human cells, and microstructures, which recapitulate essential vascular structure and physiology and allow a well-controlled microenvironment and spatial-temporal readouts. Blood vessel chips afford promising opportunities to understand molecular and cellular mechanisms underlying a range of vascular diseases. The physiological relevance is key to these ...

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Section: Vascular Physiology and On‐chip Recapitulationmentioning

confidence: 99%

“…[ 217 ] This strategy of the gradually increased flow rate can be useful for screening culture parameters and investigating vascular biology. [ 228 ]…”

Section: Vascular Physiology and On‐chip Recapitulationmentioning

confidence: 99%

Section: Shear Stressmentioning

confidence: 99%

Section: Shear Stressmentioning

confidence: 99%

See 2 more Smart Citations

Building Blood Vessel Chips with Enhanced Physiological Relevance

Gerhard‐Herman

Zhang

2023

Adv Materials Technologies

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“…For instance, to tackle the issues commonly observed in vessels developed in static conditions, such as endothelium shedding and irregular orientation, the Li group fabricated a chip by seeding ECs on a highly oriented electrospun poly(ε-caprolactone fibers) scaffold. The ECs grown on the chip showed improved endothelialization under perfusion and achieved alignment under a flow similar to natural vessel endothelium (178). Besides direct seeding cells on a scaffold, vessel-on-a-chip has also been made by fabricating cell-laden constructs and seeding cells on the hybrid constructs.…”

Section: Vessel-on-a-chipmentioning

confidence: 99%

Recent Progress in in vitro Models for Atherosclerosis Studies

Zhang

Millican

Lynd

et al. 2022

Front. Cardiovasc. Med.

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Atherosclerosis is the primary cause of hardening and narrowing arteries, leading to cardiovascular disease accounting for the high mortality in the United States. For developing effective treatments for atherosclerosis, considerable efforts have been devoted to developing in vitro models. Compared to animal models, in vitro models can provide great opportunities to obtain data more efficiently, economically. Therefore, this review discusses the recent progress in in vitro models for atherosclerosis studies, including traditional two-dimensional (2D) systems cultured on the tissue culture plate, 2D cell sheets, and recently emerged microfluidic chip models with 2D culture. In addition, advanced in vitro three-dimensional models such as spheroids, cell-laden hydrogel constructs, tissue-engineered blood vessels, and vessel-on-a-chip will also be covered. Moreover, the functions of these models are also summarized along with model discussion. Lastly, the future perspectives of this field are discussed.

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