Studying dynamic events in the developing myocardium

Vostarek, Frantisek; Šaňková, Barbora; Sedmera, David

doi:10.1016/j.pbiomolbio.2014.06.002

Cited by 10 publications

(6 citation statements)

References 98 publications

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“…Re-entry persisting for multiple beats was also observed, but none occurred at ED9.5. Rare cases of irregular rhythm (sinoatrial and AV block, alternating patterns of ventricular activation) were also observed in our large mouse series (Sankova et al 2012), while ectopics originating from the outflow tract myocardium were seen exclusively in ED10.5-ED11.5 hearts cultured for 24 h (Vostarek et al 2014). AV re-entry was observed as a rarity in one ED4 chick heart (Fig.…”

Section: Spontaneously Occurring Arrhythmias In Embryossupporting

confidence: 68%

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Arrhythmias in the developing heart

et al. 2014

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Prevalence of cardiac arrhythmias increases gradually with age; however, specific rhythm disturbances can appear even prior to birth and markedly affect foetal development. Relatively little is known about these disorders, chiefly because of their relative rarity and difficulty in diagnosis. In this review, we cover the most common forms found in human pathology, specifically congenital heart block, pre-excitation, extrasystoles and long QT syndrome. In addition, we cover pertinent literature data from prenatal animal models, providing a glimpse into pathogenesis of arrhythmias and possible strategies for treatment.

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Section: Spontaneously Occurring Arrhythmias In Embryossupporting

confidence: 68%

“…), while ectopics originating from the outflow tract myocardium were seen exclusively in ED10.5–ED11.5 hearts cultured for 24 h (Vostarek et al . ). AV re‐entry was observed as a rarity in one ED4 chick heart (Fig.…”

Section: Spontaneously Occurring Arrhythmias In Embryosmentioning

confidence: 97%

Arrhythmias in the developing heart

et al. 2014

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“…The ability to see such double upstrokes depends on good staining of both the internal and external myocardial layers, focal depth of the imaging system, and its spatial and temporal resolution. More detail from the depth of the heart could be revealed using line scan mode on a confocal microscope (Vostarek et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Trabecular Architecture Determines Impulse Propagation Through the Early Embryonic Mouse Heart

et al. 2019

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Most embryonic ventricular cardiomyocytes are quite uniform, in contrast to the adult heart, where the specialized ventricular conduction system is molecularly and functionally distinct from the working myocardium. We thus hypothesized that the preferential conduction pathway within the embryonic ventricle could be dictated by trabecular geometry. Mouse embryonic hearts of the Nkx2.5:eGFP strain between ED9.5 and ED14.5 were cleared and imaged whole mount by confocal microscopy, and reconstructed in 3D at 3.4 μm isotropic voxel size. The local orientation of the trabeculae, responsible for the anisotropic spreading of the signal, was characterized using spatially homogenized tensors (3 × 3 matrices) calculated from the trabecular skeleton. Activation maps were simulated assuming constant speed of spreading along the trabeculae. The results were compared with experimentally obtained epicardial activation maps generated by optical mapping with a voltage-sensitive dye. Simulated impulse propagation starting from the top of interventricular septum revealed the first epicardial breakthrough at the interventricular grove, similar to experimentally obtained activation maps. Likewise, ectopic activation from the left ventricular base perpendicular to dominant trabecular orientation resulted in isotropic and slower impulse spreading on the ventricular surface in both simulated and experimental conditions. We conclude that in the embryonic pre-septation heart, the geometry of the A-V connections and trabecular network is sufficient to explain impulse propagation and ventricular activation patterns.

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“…Our experimental set‐up (Vostarek et al . ) for imaging normal and stressed embryonic hearts represents a significant technological improvement. Among other advances, it enables precise localization of sites of conduction block and the uncovering of ectopic pacemakers (Hoogaars et al .…”

Section: Discussionmentioning

confidence: 99%

“…We used the calcium‐sensitive dye Rhod‐2 with a modified set‐up (Vostarek et al . ). Stained hearts were placed into a tissue bath containing 2 mL of Tyrode's solution with 0.15 μ m blebbistatin to reduce movement (Fedorov et al .…”

Section: Methodsmentioning

confidence: 97%