Study the oxidative injury of yeast cells by NADH autofluorescence

Ju, Liang; Wu, Wen-Lan; Liu, Zhihong; Mei, Yadong; Cai, Ruxiu; Shen, Ping

doi:10.1016/j.saa.2006.07.035

Cited by 35 publications

(40 citation statements)

References 15 publications

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“…Similar results of increased NADH fluorescence intensity were reported by Levitt et al 45 and by Liang et al 46 Levitt et al 45 observed that intense fluorescence was progressively confined to a gradually smaller perinuclear cytoplasmic region in the cells that had been treated with cisplatin. Their results suggest that this strongly fluorescent, highly metabolically active perinuclear ring represents a subpopulation of mitochondria that are mobilized in response to the apoptotic stimulus and may provide the energy required to execute the final step of apoptosis.…”

Section: Discussionsupporting

confidence: 81%

Differentiation of apoptosis from necrosis by dynamic changes of reduced nicotinamide adenine dinucleotide fluorescence lifetime in live cells

Wang¹,

Gukassyan²,

Chen³

et al. 2008

J. Biomed. Opt.

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Direct monitoring of cell death (i.e., apoptosis and necrosis) during or shortly after treatment is desirable in all cancer therapies to determine the outcome. Further differentiation of apoptosis from necrosis is crucial to optimize apoptosis-favored treatment protocols. We investigated the potential modality of using tissue intrinsic fluorescence chromophore, reduced nicotinamide adenine dinucleotide (NADH), for cell death detection. We imaged the fluorescence lifetime changes of NADH before and after staurosporine (STS)-induced mitochondria-mediated apoptosis and hydrogen peroxide (H2O2)-induced necrosis, respectively, using two-photon fluorescence lifetime imaging in live HeLa cells and 143B osteosarcoma. Time-lapsed lifetime images were acquired at the same site of cells. In untreated cells, the average lifetime of NADH fluorescence was approximately 1.3 ns. The NADH average fluorescence lifetime increased to approximately 3.5 ns within 15 min after 1 microM STS treatment and gradually decreased thereafter. The NADH fluorescence intensity increased within 15 min. In contrast, no significant dynamic lifetime change was found in cells treated with 1 mM H2O2. Our findings suggest that monitoring the NADH fluorescence lifetime may be a valuable noninvasive tool to detect apoptosis and distinguish apoptosis from necrosis for the optimization of apoptosis-favored treatment protocols and other clinical applications.

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Section: Discussionsupporting

confidence: 81%

Differentiation of apoptosis from necrosis by dynamic changes of reduced nicotinamide adenine dinucleotide fluorescence lifetime in live cells

Wang¹,

Gukassyan²,

Chen³

et al. 2008

J. Biomed. Opt.

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“…23 As the epidermal cells in our experiment were progressing through ischemic necrosis, the reduction in autofluorescence may represent an initial depletion of NAD͑P͒H while the cells respond to metabolic demands and antioxidant responses, which are also associated with cell death. 23,50,51 In the ex vivo skin we examined, the recovery of autofluorescence and thus NAD͑P͒H levels may be due to a temporary burst of metabolic activity stimulated by ischemic-injuryinduced damage and response systems.…”

Section: Discussionmentioning

confidence: 85%

“…22 Ischemia has been well described to cause a physical reduction in NAD͑P͒H and a corresponding decrease in autofluorescence in a variety of cells and tissues. 23,45,46 The correlation with NADH autofluorescence intensity is related 47 to the physical concentration of the NADH. NADH autofluorescence has been used to assess the onset of skin flap necrosis in rats and showed a significant decrease in autofluorescence with time and necrosis.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of the metabolic deterioration of ex vivo skin from ischemic necrosis through the imaging of intracellular NAD(P)H by multiphoton tomography and fluorescence lifetime imaging microscopy

et al. 2010

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Abstract. Ex vivo human skin has been used extensively for cosmeceutical and drug delivery studies, transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to ischemic necrosis. Traditional methods of assessing viability can be timeconsuming and provide limited metabolic information. Using multiphoton tomography and fluorescence lifetime imaging ͑MPT-FLIM͒ we assess ischemic necrosis of ex vivo skin by NAD͑P͒H autofluorescence intensity and fluorescence lifetime. Ex vivo skin is stored in the presence and absence of nutrient media ͑Dulbecco Modified Eagle Medium͒ at −20, 4, and 37°C and room temperature over a 7-day time course to establish different rates of metabolic deterioration. At higher temperatures we observe a decrease in NAD͑P͒H autofluorescence, higher image noise, and a significant increase in the average fluorescence lifetime ͑ m ͒ from ϳ1000 to 2000 ps. Additionally, significant distortions in NAD͑P͒H fluorescence lifetime histograms correspond to the reduction in autofluorescence. Skin kept at 4°C, with or without media, showed the least change. Our findings suggest that MPT-FLIM enables useful noninvasive optical biopsies to monitor the metabolic state and deterioration of human skin for research and clinical purposes.

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“…This maximum of NADH fluorescence is usually observed in living cells (Andersson et al, 1998). The 30-nm shift of the emission intensity peak with respect to the standard NADH solution has already been described as a normal phenomenon due to the cellular environment (Liang et al, 2007).…”

Section: Gr24 Rapidly Increases Nadph Synthesis In Gigaspora Roseamentioning

confidence: 89%

GR24, a Synthetic Analog of Strigolactones, Stimulates the Mitosis and Growth of the Arbuscular Mycorrhizal FungusGigaspora roseaby Boosting Its Energy Metabolism

et al. 2008

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Arbuscular mycorrhizal (AM) fungi are obligate biotrophs that participate in a highly beneficial root symbiosis with 80% of land plants. Strigolactones are trace molecules in plant root exudates that are perceived by AM fungi at subnanomolar concentrations. Within just a few hours, they were shown to stimulate fungal mitochondria, spore germination, and branching of germinating hyphae. In this study we show that treatment of Gigaspora rosea with a strigolactone analog (GR24) causes a rapid increase in the NADH concentration, the NADH dehydrogenase activity, and the ATP content of the fungal cell. This fully and rapidly (within minutes) activated oxidative metabolism does not require new gene expression. Up-regulation of the genes involved in mitochondrial metabolism and hyphal growth, and stimulation of the fungal mitotic activity, take place several days after this initial boost to the cellular energy of the fungus. Such a rapid and powerful action of GR24 on G. rosea cells suggests that strigolactones are important plant signals involved in switching AM fungi toward full germination and a presymbiotic state.

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Study the oxidative injury of yeast cells by NADH autofluorescence

Cited by 35 publications

References 15 publications

Differentiation of apoptosis from necrosis by dynamic changes of reduced nicotinamide adenine dinucleotide fluorescence lifetime in live cells

Differentiation of apoptosis from necrosis by dynamic changes of reduced nicotinamide adenine dinucleotide fluorescence lifetime in live cells

Analysis of the metabolic deterioration of ex vivo skin from ischemic necrosis through the imaging of intracellular NAD(P)H by multiphoton tomography and fluorescence lifetime imaging microscopy

GR24, a Synthetic Analog of Strigolactones, Stimulates the Mitosis and Growth of the Arbuscular Mycorrhizal FungusGigaspora roseaby Boosting Its Energy Metabolism

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