Study on the relationship between genetic variation of DNA methylation and heterosis in soybean leaves

Wang, Yuanqian; Zhang, Kaixin; Sun, Lifang; Han, Xia; Fan, Sujie; Li, Xueying; Qu, Yiwei; Yao, Dan; Wang, Piwu; Zhang, Jun

doi:10.1007/s10681-018-2161-z

Cited by 9 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among them, methylation through promoter region is an effective way to inhibit gene expression without DNA sequence variation ( Maunakea et al, 2010 ). These make more and more researchers believe that there must be a correlation between heterosis and DNA methylation, and begin to explain the heterosis mechanisms from the aspect of epigenetic ( Groszmann et al, 2013 ; Xiong et al, 2013 ; Wang et al, 2018 ). To ascertain the methylation level of EjGIF1 promoter among triploid loquats and their parents, we have analyzed the methylation levels in three randomly selected triploid loquats and their parents by bisulfite sequencing.…”

Section: Discussionmentioning

confidence: 99%

Functional Identification of EjGIF1 in Arabidopsis and Preliminary Analysis of Its Regulatory Mechanisms in the Formation of Triploid Loquat Leaf Heterosis

et al. 2021

View full text Add to dashboard Cite

Although several results have been obtained in triploid loquat heterosis (i.e., leaf size of triploid loquat) studies in the past years, the underlying mechanisms of the heterosis are still largely unknown, especially the regulation effects of one specific gene on the corresponding morphology heterosis. In this study, we sought to further illustrate the regulatory mechanisms of one specific gene on the leaf size heterosis of triploid loquats. A leaf size development-related gene (EjGIF1) and its promoter were successfully cloned. Ectopic expression of EjGIF1 in Arabidopsis showed that the leaf size of transgenic plantlets was larger than that of WTs, and the transgenic plantlets had more leaves than WTs. Quantitative Reverse Transcription PCR (qRT-PCR) showed that the expression level of EjGIF1 showed an AHP expression pattern in most of the hybrids, and this was consistent with our previous phenotype observations. Structure analysis of EjGIF1 promoter showed that there were significantly more light-responsive elements than other elements. To further ascertain the regulatory mechanisms of EjGIF1 on triploid loquat heterosis, the methylation levels of EjGIF1 promoter in different ploidy loquats were analyzed by using bisulfite sequencing. Surprisingly, the total methylation levels of EjGIF1 promoter in triploid showed a decreasing trend compared with the mid-parent value (MPV), and this was also consistent with the qRT-PCR results of EjGIF1. Taken together, our results suggested that EjGIF1 played an important role in promoting leaf size development of loquat, and demethylation of EjGIF1 promoter in triploid loquats caused EjGIF1 to exhibit over-dominance expression pattern and then further to promote leaf heterosis formation. In conclusion, EjGIF1 played an important role in the formation of triploid loquat leaf size heterosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Functional Identification of EjGIF1 in Arabidopsis and Preliminary Analysis of Its Regulatory Mechanisms in the Formation of Triploid Loquat Leaf Heterosis

et al. 2021

View full text Add to dashboard Cite

show abstract

“… Nakamura and Hosaka (2010) observed that the levels of methylation were significantly different in the parents and hybrid potatoes and also stated that homozygosity and heterozygosity of the methylated DNA are known to regulate heterosis and inbreeding depression, respectively. Wang et al (2018) studied that the methylation-sensitive amplification polymorphism (MSAP) method using the capillary electrophoresis was used for the estimation of cytosine methylation in the 15-day post-emergence leaves of parental and hybrid lines and reported that hypermethylation can be used for increasing the stem diameter. In contrast, Sun et al (2015) and Kawanabe et al (2016) showed that there is a relationship between heterosis and methylation of DNA.…”

Section: Generation Of Heterosis and Male Sterility In Soybeanmentioning

confidence: 99%

Harnessing heterosis and male sterility in soybean [Glycine max (L.) Merrill]: A critical revisit

et al. 2022

View full text Add to dashboard Cite

Soybean is a predominantly self-pollinated crop. It is also one of the important oilseed legumes. Soybean is an excellent crop having industrial, traditional, culinary, feeding, and cultural roles. Genetic diversity in breeding programs is of prime importance as it ensures the success of any breeding by enhancing the outcomes and results of the plants. The phenomenon wherein the progeny exhibits greater biomass (yield) and a faster rate of development and fertility than its parents is referred to as heterosis. As of now, heterosis is mainly limited to the trait of seed yield and is considered the basis for the development of better (superior) varieties. Male sterility (MS) is extensively used for the production of seeds and the improvement of crops coupled with the traditional breeding programs and molecular technology. Therefore, deployment of MS and heterosis in breeding soybean could yield better outcomes. This review aims to focus on two aspects, namely, MS and heterosis in soybean with its scope for crop improvement.

show abstract

“…Some methylation variations change the transcription level, which play a role in the increase in biomass heterosis ( Greaves et al, 2012 ). Wang et al (2018b) analyzed soybean hybrids by using MSAP and found that the increase in node number is promoted by hypomethylation, and the stem diameter of hybrids can be increased by hypermethylation. More interestingly, the methylation level of alleles from both parents will change when the DNA methylation level greatly varies.…”

Section: Epigenetics and Plant Heterosismentioning

confidence: 99%

Advances in Research on the Mechanism of Heterosis in Plants

Liu

Zhang

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

Heterosis is a common biological phenomenon in nature. It substantially contributes to the biomass yield and grain yield of plants. Moreover, this phenomenon results in high economic returns in agricultural production. However, the utilization of heterosis far exceeds the level of theoretical research on this phenomenon. In this review, the recent progress in research on heterosis in plants was reviewed from the aspects of classical genetics, parental genetic distance, quantitative trait loci, transcriptomes, proteomes, epigenetics (DNA methylation, histone modification, and small RNA), and hormone regulation. A regulatory network of various heterosis-related genes under the action of different regulatory factors was summarized. This review lays a foundation for the in-depth study of the molecular and physiological aspects of this phenomenon to promote its effects on increasing the yield of agricultural production.

show abstract

Study on the relationship between genetic variation of DNA methylation and heterosis in soybean leaves

Cited by 9 publications

References 33 publications

Functional Identification of EjGIF1 in Arabidopsis and Preliminary Analysis of Its Regulatory Mechanisms in the Formation of Triploid Loquat Leaf Heterosis

Functional Identification of EjGIF1 in Arabidopsis and Preliminary Analysis of Its Regulatory Mechanisms in the Formation of Triploid Loquat Leaf Heterosis

Harnessing heterosis and male sterility in soybean [Glycine max (L.) Merrill]: A critical revisit

Advances in Research on the Mechanism of Heterosis in Plants

Contact Info

Product

Resources

About