Study on the Function of miR-134 on Cognitive Function of Vascular Dementia (VD) Rats and Mechanism About Oxidative Stress and Autophagy and Cofilin 2 Level

Abstract

To discuss the mechanism of miR-134 in improving cognitive function of VD rats through regulating the oxidative stress and autophagy and reducing the expression of Cofilin 2. VD rats was established. They were disposed with miR-134 antagonist. The cerebral regulatory capacity was observed through ethology. The pathological change in CAI area of hippocampus and cerebral cortex was observed with HE staining method. The regulation of miR-134 targeting downstream was analyzed through bioinformatics. The presentation level of SOD, GSH, ROS and MDA was detected. The expression of LC1/LC-3 and p62 was detected with Western Blot assay. There was visible activated microglial cells and gliocyte proliferation in VD rat’s model. The myelination was weakened. They were improved notably through the treatment with miR-134 antagonist. The expression of MDA and ROS could be restrained by miR-134 antagonist through reducing the expression of Cofilin 2. The expression of SOD and GSH could be increased and oxidative stress could be reduced. The level of autophagy could be decreased. The cognitive function of VD rats could be improved by miR-134 antagonist through regulating the oxidative stress and autophagy and reducing presentation of Cofilin 2.

Exploring the Regulatory Landscape of Dementia: Insights from Non-Coding RNAs

“…Additionally, miR-134 was found to decrease Cofilin 2 levels, thereby regulating oxidative stress and autophagy in the brain. Suppression of miR-134 in VD rats improved cognitive function by modulating oxidative stress and autophagy, resulting in induced levels of Cofilin 2 [ 121 ]. Another study revealed that downregulated miR-181a can increase PTEN-induced kinase 1 ( PINK1 )/Parkin expression and enhanced mitophagy to improve mitochondrial function and enhance cognitive capabilities in VD.…”