Study on structure-function of starch by asymmetrical flow field-flow fractionation coupled with multiple detectors: A review

Guo, Panpan; Li, Yueqiu; An, Jinxuan; Shen, Shigang; Dou, Haiyang

doi:10.1016/j.carbpol.2019.115330

Cited by 34 publications

(22 citation statements)

References 120 publications

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“…The M and r G in Table 3 are in good agreement with those reported in literature where GA was analyzed by size exclusion chromatography (SEC) or AF4 coupled with MALS [28,45], according to which the P-1 and P-2 correspond to arabinogalactan (AG) and arabinogalactan protein (AGP), respectively [28,38,45,46]. The P-3 is likely to be composed of aggregates that could be formed by the self-assembly of AGP and is known to be present in plant secretions containing AGP [32].…”

Section: Analysis Of Labeled Ga By Af4-mals-dri-flsupporting

confidence: 89%

“…Asymmetrical flow field-flow fractionation (AF4) is a technique with a broad separation range in size and gentle conditions, making it well-suited for polydisperse polysaccharides [29][30][31][32][33]. Pre-separation fluorescence labeling followed by AF4 separation and fluorescence detection has been used to characterize protein in polysaccharides such as GA, starch, β-glucan, and mesquite gum [28,34,35].…”

Section: Introductionmentioning

confidence: 99%

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Modification of EDC method for increased labeling efficiency and characterization of low-content protein in gum acacia using asymmetrical flow field-flow fractionation coupled with multiple detectors

et al. 2021

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Abstract1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) is widely used as a crosslinker for fluorescence labeling of protein in the fields of biochemistry and food analysis. Many natural polysaccharides often contain some proteins or peptides that are very low in content but play a vital role in their biological function as well as technical applications. Determination of these low-content proteinaceous matters requires a highly sensitive and selective method. In this study, a methodological approach for investigations of the presence of proteinaceous material over the molar mass distribution (MD) of polysaccharides was developed using gum acacia (GA) as a model polysaccharide. EDC fluorescence-labeling method was modified by changing the pH (7, 9, and 11) of the solution for the analysis of low-content protein in food materials. Fluorescence spectroscopy and asymmetrical flow field-flow fractionation (AF4) were employed for characterizing the labeling efficiency and physiochemical properties of unlabeled and fluorescence-labeled GA. AF4 provided molar mass (M) and the radius of gyration (rG) of arabinogalactan (AG) and arabinogalactan protein complex (AGP) and determined the presence of proteinaceous matter over the MD. The labeling efficiencies of GA at pH 7, 9, and 11 determined by fluorescence spectroscopy were 56.5, 68.4, and 72.0%, respectively, with an increment of 15.5% when pH was increased from 7 to 11. The modified EDC fluorescence-labeling method allows highly sensitive and selective analysis of low-content proteinaceous matters and their distribution in natural polysaccharides. Graphical abstract

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Section: Analysis Of Labeled Ga By Af4-mals-dri-flsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Modification of EDC method for increased labeling efficiency and characterization of low-content protein in gum acacia using asymmetrical flow field-flow fractionation coupled with multiple detectors

et al. 2021

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“…is discrepancy may be ascribed to the increased amylopectin content of waxy wheat flour, which significantly affects water absorption [49]. Amylopectin is mainly located in the crystallization region of starch granules, which have strong water absorption and high water holding capacity [50].…”

Section: Farinograph Properties Of Dough Dough Rheologicalmentioning

confidence: 99%

Study of Noodle Quality Based on Protein Properties of Three Wheat Varieties

Zhou

Sun

Yao

et al. 2022

Journal of Food Quality

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Waxy wheat offers unique benefits in food processing, including improving the smoothness and performance of the product. However, waxy wheat is not yet commercially available. The protein characteristics, including the protein content, subunit distribution, secondary structure, chemical interactions, and microstructure of the gluten, were explored to realize the full potential of waxy wheat. The results showed that the noodles prepared from waxy wheat had a gentle and glutinous texture compared with GY2018 and YM13. Partial-waxy and waxy wheat had a lower gluten index and glutenin macropolymer (GMP) content than GY2018, indicating a reduced gluten strength. Confocal laser scanning microscopy (CLSM) images showed that the starch granules were not securely attached to the partial-waxy and waxy wheat protein matrix. In addition, the waxy protein chains appeared more elongated and they weakened the protein network. In particular, HMW-GS subunit 2 + 12 may be the essential cause of the weak dough from SKN1. Compared with GY2018 and YM13, SKN1 had the highest number of free sulfhydryl groups. Rather than ionic bonds, hydrophobic interactions increased the gluten network in GY2018, YM13, and SKN1. The weak molecular forces in the gluten will result in a soft noodle texture.

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“…Pati telah banyak digunakan dalam perindustrian seperti makanan, biomedis, pembuatan kertas, dan perekat kayu. Pati terdiri dari dua jenis polisakarida, yaitu amilosa dan amilopektin [12]. Gambar 2.…”

Section: Patiunclassified

Perbandingan Plastik Dari Material-Material Bioplastik

Rosmainar¹,

Tukan²,

Deviyanti³

2021

JJMS

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Plastik merupakan suatu polimer yang mempunyai sifat unik dan merupakan benda yang luar biasa. Polimer merupakan bahan yang terdiri dari molekul-molekul yang disebut dengan monomer dengan jenis homopolimer. Polimer biasa dikenal dalam kehidupan sehari-hari seperti selulosa, protein, karet dan bahan alam lainnya. Peningkatan penggunaan plastik merupakan dampak dari berkembangnya dunia perindustrian, teknologi dan meningkatnya jumlah penduduk dunia. Di Indonesia, kebutuhan plastik terus mengalami peningkatan dengan rata-rata 200 ton per tahun. Pada tahun 2002 telah tercatat sekitar 1,9 juta ton sampah plastik, dan ditahun 2011 jumlah sampah plastik meningkat menjadi 2,6 juta ton pertahun. Berdasarkan asumsi Kementrian Lingkungan Hidup (KLH), setiap hari penduduk Indonesia dapat mengahsilkan 0,8 kg sampah per orang dan jika ditotal maka sebanyak 189 ribu ton sampah per hari. Sampah yang dihasilkan sebanyak 15% berupa sampah plastik atau sekitar 28,4 ribu ton per hari. Pada awalnya polimer alam digunakan sebagai bahan pembuatan perkakas dan pembuatan senjata. Monomer dalam pembentukan polimer merupakan molekul yang memiliki ikatan rangkap atau yang memiliki gugus fungsi tertentu. Polimer digolongkan atas polimer alam dan polimer sintetik. Polimer alam seperti selulosa, pati, kitin, kitosan, gelatin, dekstran, alginat, pektin, gum guar dan karet digunakan dalam pembuatan nanokomposit. Polimer alam menunjukkan sifat seperti kemurnian, kristalinitas, solditas tarik, meningkatkan elastisitas dan memilikki permukaan yang luas. Kesimpulannya adalah daya tarik pada bioplastik memiliki kekuatan yang sama namun jika ditambahkan dengan material-material yang lain maka daya hantar akan semakin kuat dan semakin baik.

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Study on structure-function of starch by asymmetrical flow field-flow fractionation coupled with multiple detectors: A review

Cited by 34 publications

References 120 publications

Modification of EDC method for increased labeling efficiency and characterization of low-content protein in gum acacia using asymmetrical flow field-flow fractionation coupled with multiple detectors

Modification of EDC method for increased labeling efficiency and characterization of low-content protein in gum acacia using asymmetrical flow field-flow fractionation coupled with multiple detectors

Study of Noodle Quality Based on Protein Properties of Three Wheat Varieties

Perbandingan Plastik Dari Material-Material Bioplastik

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