Study on separative structure of EnFET to detect acetylcholine

Chi, Li-Lun; Yin, Li-Te; Chou, Jung-Chuan; Chung, Wen-Yaw; Sun, Tai‐Ping; Hsiung, Kuang-Pin; Hsiung, Shen‐Kan

doi:10.1016/s0925-4005(00)00606-7

Cited by 59 publications

(35 citation statements)

References 16 publications

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“…BioFETs can be subcategorised depending upon the recognition element used: Enzyme FETs (EnFETs) make use of enzymes to provide the bioselectivity (Chi et al, 2000;Dzyadevich et al, 1999;Luo, et al, 2004Luo, et al, , 2004bPoghossian et al, 2001;Wan et al, 1999); Immunological FETs (ImmunoFETs) make use of antibodies (Sekiguchi et al, 2000;Sergeyeva et al, 1999;Starodub et al, 2000); and Cell Potential FETs (CPFETs) (Baumann et al, 1999) are able to measure the properties of whole cells positioned on top of the gate. In the case of oligonucleotide sensing, cDNA capture probes can be bound to the surface of an ISFET to create a geneFET (Fig.…”

Section: Genefetsmentioning

confidence: 99%

Micro- and nano-structure based oligonucleotide sensors

Ferrier

Shaver

Hands

2015

Biosensors and Bioelectronics

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This paper presents a review of micro- and nano-structure based oligonucleotide detection and quantification techniques. The characteristics of such devices make them very attractive for Point-of-Care or On-Site-Testing biosensing applications. Their small scale means that they can be robust and portable, their compatibility with modern CMOS electronics means that they can easily be incorporated into hand-held devices and their suitability for mass production means that, out of the different approaches to oligonucleotide detection, they are the most suitable for commercialisation. This review discusses the advantages of micro- and nano-structure based sensors and covers the various oligonucleotide detection techniques that have been developed to date. These include: Bulk Acoustic Wave and Surface Acoustic Wave devices, micro- and nano-cantilever sensors, gene Field Effect Transistors, and nanowire and nanopore based sensors. Oligonucleotide immobilisation techniques are also discussed.

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Section: Genefetsmentioning

confidence: 99%

Micro- and nano-structure based oligonucleotide sensors

Ferrier

Shaver

Hands

2015

Biosensors and Bioelectronics

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“…A configuration of a SnO 2 /ITO/glass substrate was studied [17,[27][28][29][30]. The SnO 2 /ITO/glass substrate comprises two elements-indium tin oxide (ITO) that was supplied by the Wintek Corporation, and SnO 2 thin films, which was prepared using a radio frequency (r.f.)…”

Section: Preparation Of Ammonium Ion-selective Membranementioning

confidence: 99%

“…Recently, the potentiometric sensor has been widely studied using semiconductor-based device, such as ion-sensitive field effect transistor (ISFET) [8][9][10], extended gate field effect transistor (EGFET) [11], metal-oxide electrode [12][13][14] and potentiometric biosensor [15][16][17]. However, the requirement for a reference electrode is difficult to meet while exploiting planar or solid-state technology on a single device.…”

Section: Introductionmentioning

confidence: 99%

Differential type solid-state urea biosensors based on ion-selective electrodes

Chou

Sun

et al. 2008

Sensors and Actuators B: Chemical

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“…Their presence changes the potential of the reaction medium, which can be measured potentiometrically. 6 Thus, it is possible to directly detect ACh 7 and indirectly detect a class of pesticides by measuring enzyme inhibition.…”

Section: Introductionmentioning

confidence: 99%

Self-Assembly of SiO<SUB>2</SUB> Nanoparticles for the Potentiometric Detection of Neurotransmitter Acetylcholine and Its Inhibitor

Arruda¹,

Guimarães²,

Ramos³

et al. 2014

j nanosci nanotechnol

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The detection and quantification of neurotransmitter acetylcholine (ACh) are relevant because modifications in the ACh levels constitute a threat to human health. The biological regulator of this neurotransmitter is acetylcholinesterase (AChE), an enzyme that catalyzes the hydrolysis of ACh to choline and acetic acid. However, its activity is inhibited in the presence of organophosphate and carbamate pesticides, compromising the degradation of the neurotransmitter. There has been a growing interest in faster and more sensitive detection systems that include new methods and materials for the determination of the ACh concentration. This paper proposes a potentiometric biosensor for the detection of neurotransmitter ACh and its inhibitors, specifically organophosphate pesticide methamidophos. The biosensor is based on a self-assembled platform formed by poly(allylamine) hydrochloride (PAH) and silicon dioxide nanoparticles (SiO 2 -Np) that contains the immobilized enzyme AChE. First, the responses of the biosensor were investigated for different concentrations of ACh in buffer solutions. After quantifying ACh, the inhibition of AChE in the presence of methamidophos was determined, enabling the quantification of methamidophos expressed as the percentage of enzyme inhibition. The potential advantages of this biosensor include simplicity in building the electrode, possible production on an industrial scale, limited need for qualified personnel to operate the device and low processing cost.

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Study on separative structure of EnFET to detect acetylcholine

Cited by 59 publications

References 16 publications

Micro- and nano-structure based oligonucleotide sensors

Micro- and nano-structure based oligonucleotide sensors

Differential type solid-state urea biosensors based on ion-selective electrodes

Self-Assembly of SiO<SUB>2</SUB> Nanoparticles for the Potentiometric Detection of Neurotransmitter Acetylcholine and Its Inhibitor

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