Study on colorectal cancer presentation, treatment and follow-up

Aakif, Muhammad; Balfe, Paul; Elfaedy, Osama; Awan, Faisal; Pretorius, Friedrick; Silvio, Leonardo; Castinera, Constantino; Hamid, Mohammad

doi:10.1007/s00384-015-2479-0

Cited by 13 publications

(11 citation statements)

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“…Colorectal cancer is one of the most common digestive tract malignancies worldwide, with a poor prognosis ( 23 ). Apoptosis or programmed cell death (PCD) is a mechanism of nucleated cell death, which is regulated by physiological processes, including alterations to the intracellular and extracellular environments or cell death signaling activation.…”

Section: Discussionmentioning

confidence: 99%

Inï¿½vivo and inï¿½vitro induction of the apoptotic effects of oxysophoridine on colorectal cancer cells via the Bcl‑2/Bax/caspase-3 signaling pathway

Jin

Yang

et al. 2017

Oncol Lett

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Oxysophoridine (OSR) is a major active alkaloid extracted from Sophoraalopecuroides L. The aim of the present study was to investigate the induction of the apoptotic effects of OSR on colorectal cancer cells in vivo and in vitro. The results of the MTT and colony formation assays demonstrated that the proliferation of HCT116 cells was inhibited by OSR in vitro. The characteristics of cellular apoptosis in OSR-treated HCT116 cells were analyzed by Hoechst 33258 staining. It was also observed that the expression of caspase-3, B-cell lymphoma-2 (Bcl-2) associated X protein (Bax) and cytochrome c increased significantly upon OSR treatment. However, the expression of Bcl-2 and poly ADP-ribose polymerase-1 (PARP-1) was downregulated in OSR-treated cells compared with untreated cells. The in vivo experiments identified that OSR significantly inhibited the growth of the transplanted mouse CT26 tumor tissue, upregulated the expression of caspase-3, Bax and cytochrome c and downregulated the expression of Bcl-2 and PARP-1, as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. It may be concluded that OSR significantly induced apoptotic effects on colorectal cancer cells in vivo and in vitro, and that its mechanism may be associated with the Bcl-2/Bax/caspase-3 signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Inï¿½vivo and inï¿½vitro induction of the apoptotic effects of oxysophoridine on colorectal cancer cells via the Bcl‑2/Bax/caspase-3 signaling pathway

Jin

Yang

et al. 2017

Oncol Lett

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“…Patients who have been diagnosed with early stage CRC frequently are treated with surgery in conjunction with chemotherapy and radiotherapy to improve their survival rate (1). However, for the majority of patients diagnosed with CRC have already reached an advanced pathological stage, chemotherapy is the major form of treatment (5)(6)(7)(8). In particular, 5-fluorouracil (5-FU)-based regimens are used as the international standard for late-stage CRC treatment (9,10).…”

Section: Introductionmentioning

confidence: 99%

Hedyotis diffusa willd extract suppresses colorectal cancer growth through multiple cellular pathways

et al. 2017

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Abstract. The development of colorectal cancer (CRC) is strongly associated with the imbalance of various intracellular signal transduction cascades, including protein kinase B (AKT), mitogen-activated protein kinase 1 (MAPK), signal transducer and activator of transcription 3 (STAT3), as well as crosstalk between these signaling networks. At present, anti-tumor agents are often single-targeted and therefore are not always therapeutically effective. Moreover, long-term use of these anti-tumor agents often generates drug resistance and potential side effects. These problems highlight the urgent need for the development of novel and more effective anti-cancer drugs. Hedyotis diffusa Willd (HDW) has been used as a major component in traditional Chinese medicine for the clinical treatment of colorectal cancer, with a limited number of adverse effects. However, the molecular mechanisms, which underlie its anti-cancer activity, still require further elucidation. In the present study, using xenograft models and various different human CRC cell lines, the efficacy of the ethanol extract of HDW (EEHDW) against tumor growth was evaluated, and its underlying molecular mechanisms of action were investigated. It was demonstrated that EEHDW was able to inhibit cancer growth in vivo and in vitro. Furthermore, EEHDW was able to suppress the activation of several CRC-associated signaling pathways and was able to regulate the expression of various inflammatory and angiogenic factors. This resulted in the induction of apoptosis and inhibition of cellular proliferation, as well as tumor angiogenesis. The present study demonstrated that EEHDW is able to exhibit anti-cancer activity due to its ability to affect multiple intracellular targets, which suggests that it may be a novel multi-potent therapeutic agent for the treatment of colorectal cancer.

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“…Colorectal cancer (CRC) ranked the third of cancer incidence and the fourth of cancer death in 2013 [ 1 ]. A large number of deaths occurred in CRC patients owing to no effective approach for the cure of advanced CRC [ 2 , 3 ]. Although an increasing number of oncogenes have been reported to be responsible for the development of CRC, such as S100P [ 4 ], K-Ras [ 5 ] and BRAF [ 6 ], efforts are still needed to clarify the molecular mechanisms of migration and invasion of advanced CRC.…”

Section: Introductionmentioning

confidence: 99%

Pinin facilitated proliferation and metastasis of colorectal cancer through activating EGFR/ERK signaling pathway

Wei

et al. 2016

Oncotarget

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Increasing emphasis has been put on the influence of desmosome related proteins on progress of colorectal cancer (CRC). Pinin (PNN) is a desmosome-associated molecule that has been reported its overexpression could increase desmoglein 2 (DSG2) and E-cadherin (E-ca) levels. However, it was documented that DSG2 and E-ca had opposite functions in CRC. Thus, we attempted to elucidate function and mechanism of PNN in CRC. Herein, we revealed that overexpression of PNN was significantly correlated with the aggressive characteristics and indicated poor overall survival of CRC patients. In addition, the proliferation, invasion in vitro, and tumorigenic growth, metastasis in vivo were also promoted by the up-regulation of PNN. It was also verified that up-regulation of PNN increased the expression of DSG2 and activated the EGFR/ERK signaling pathway. Our findings suggested that PNN, as a valuable marker of prognosis, has important influence on the progression of CRC.

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Study on colorectal cancer presentation, treatment and follow-up

Abstract: Our study shows that the mean age group at risk for colorectal cancer is 65 years (range 54-75). Still, 33 % of patients present to acute surgical assessment units with advanced disease. Though we did well in terms of operative resections, follow-up still remains a challenge.

Cited by 13 publications

References 0 publications

Inï¿½vivo and inï¿½vitro induction of the apoptotic effects of oxysophoridine on colorectal cancer cells via the Bcl‑2/Bax/caspase-3 signaling pathway

Inï¿½vivo and inï¿½vitro induction of the apoptotic effects of oxysophoridine on colorectal cancer cells via the Bcl‑2/Bax/caspase-3 signaling pathway

Hedyotis diffusa willd extract suppresses colorectal cancer growth through multiple cellular pathways

Pinin facilitated proliferation and metastasis of colorectal cancer through activating EGFR/ERK signaling pathway

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