Study of vitamin D3 formulation prepared by phytosolve technique and its effect on CTRP6 gene expression in PCOS model

Hakimpour, Sahar; Jelodar, Gholamali; Shabani, Ronak; Pourheydar, Bagher; Ajdary, Marziyeh; Mehdizadeh, Mehdi

doi:10.1016/j.jddst.2022.103489

Cited by 8 publications

(8 citation statements)

References 26 publications

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“…The average particle size in the PhytoSolve formulation was 71.10±1.100 nm, and after 120 hours, 91.5% of the vitamin D3 had been released from it ( Hakimpour et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

“…PCOS model confirmation was done based on the method presented in our previous study and based on body weight, number of follicles, and insulin and testosterone levels ( Hakimpour et al., 2022 ).…”

Section: Resultsmentioning

confidence: 99%

“…The preparation of PhytoSolve containing VD3 was carried out according to our previous study ( Hakimpour et al ., 2022 ). Briefly, 20 mg of VD3 in 2 g of medium-chain triglyceride (MCT) oil, and 0.5g of phospholipid (Lipoid S75) were homogenized in 7.5g of polyol phase at room temperature with an Ultra-Turrax homogenizer (IKA T10B, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…The final mixture was sonicated with a probe sonicator (Hielscher, Germany) at 70% intensity for 10 minutes to reduce the emulsion particle size. The PhytoSolve formulation was prepared using polyol phase, and the physicochemical properties of the PhytoSolve containing VD3 were described in our previous study ( Hakimpour et al ., 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Induction of PCOS in mice was performed according to the protocol described in our recent study ( Hakimpour et al ., 2022 ). Briefly, a subcutaneous injection of a mixture of dehydroepiandrosterone (DHEA), sesame oil, and 95% ethanol was administered for 20 days.…”

Section: Methodsmentioning

confidence: 99%

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The effect of vitamin D3 provided with Phytosolve on the genes involved in implantation in the PCOS model

Hakimpour,

Govahi,

Eghbali

et al. 2023

JBRA

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Objective Vitamin D3 has been shown to be effective in the treatment of PCOS. However, due to its poor solvability and bioavailability, effective time is delayed and dosage requirements are increased. In our previous study, we demonstrated that PhytoSolve containing VD3 is more effective than vitamin D3 alone in the treatment of PCOS. In this study, we aimed to investigate the effect of this vitamin D3 formulation on gene expression involved in implantation in patients with PCOS. Methods To create PhytoSolve, Lipid S75, glycerol, and MCT oil were combined using a sonicator probe. Six groups, each consisting of 36 female Naval Medical Research Institute (NMRI) mice, were included in the following groups: control; sham; PCOS; PhytoSolve; PhytoSolve containing VD3; and vitamin D3. The mice were given DHEA injections to induce PCOS. After administering PhytoSolve containing VD3 and vitamin D3 by gavage for one week from the 13 th day of model creation, the female mice were mated and endometrial tissue was collected for analysis of LIF, β-integrin, and HOXA10 proteins and genes. Results Compared to the group receiving vitamin D3 alone, the group receiving PhytoSolve containing vitamin D3 showed a significant increase in the expression of LIF, β-integrin, and HOXA10 genes ( p <0.05). Although there was an increase in the expression of β-integrin and HOXA10 proteins in the group given PhytoSolve containing vitamin D3 compared to the group given vitamin D3, this increase was not significant. However, the increase in LIF protein expression in the group given PhytoSolve containing vitamin D3 was significant when compared to the group given vitamin D3 ( p <0.05). Conclusions The use of PhytoSolve containing vitamin D3 was more effective than vitamin D3 alone. The PhytoSolve formulation might be a useful solution for medications with limited solubility and bioavailability.

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“…The average particle size in the PhytoSolve formulation was 71.10±1.100 nm, and after 120 hours, 91.5% of the vitamin D3 had been released from it ( Hakimpour et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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