Study of the Myb-factor polymorphism based on comparative genomics of vegetable Solanaceae crops (tomato, pepper, eggplant) to search for DNA markers that differentiate samples by the anthocyans accumulation

Abstract

Anthocyanins are high-value plant antioxidants; they also determine biotic and abiotic stress resistance. The aim of our research was to study the allelic polymorphism of Antocyanin 1 orthologs in the vegetable Solanaceae crops of C. annuum and S. melongena. The search revealed the following closest genes in C. annuum: Myb113-like1 TF and Myb113like2 transcription factors and Myb1 in S. melongena. Exon amplicons of those genes were obtained and then sequenced in the pepper and eggplant samples with contrasting anthocyanin fruit coloration. Primers to the identified polymorphisms were developed and their correlation with the anthocyanin accumulation in fruits was studied. A close correlation was found between a minimum accumulation or the complete absence of anthocyanin synthesis in fruits with a single nucleotide deletion (Myb113-like1), and in the pepper samples, 2 SNP (Myb113-like2) was detected using the CAPS marker Myb 113-AccI. In the eggplant samples, the deletions of 6 and 26 bp were detected using the SCAR marker MybMel and the CAPS marker MybmelPst1. The disturbance of anthocyanin synthesis in pepper forms with 1Indel in Myb113-like1 TF was determined by a shift in the reading frame and SNPs in Myb113-like2 TF lead to amino acid substitutions: Lys → Arg and Thr → Lys. In the eggplant, a deletion of 6 bp leads to the loss of ala and arg in the protein; a deletion of 26 bp causes disorder during the mRNA maturation. The developed markers allow identifying the Myb-like TF alleles under study, resulting in anthocyanin synthesis disturbance in fruits. C. annuum and S. melongena samples with different alleles were selected for a further study and new varieties in agriculture.

QTL Analysis of the Content of Some Bioactive Compounds in Brassica rapa L. Grown under Light Culture Conditions

“…Accumulating in both vegetative and generative organs of plants, they cause red, pink, purple and blue coloration. It is also known that anthocyanins perform structural, protective, and signaling functions in plants, determine resistance to abiotic and biotic stresses, and participate in respiration and photosynthesis [51,52]. The study of the mechanisms of accumulation of anthocyanins in plant tissues makes it possible to determine the methods of plant adaptation to unfavorable environmental conditions and to understand the physiological and biochemical mechanisms of their resistance.…”

Genome-Wide Exploration of the WD40 Gene Family in Eggplant (Solanum melongena L.) and Analysis of Its Function in Fruit Color Formation

“…The MBW complex also plays an important role in fruit coloration in eggplant, with the MYB transcription factor being the most intensively studied. MYB transcription factors such as SmMYB1, SmMYB2, SmMYB75, SmMYB44, SmMYB86, and SmMYB35 have been identified to contribute to anthocyanin synthesis and accumulation in eggplant [35][36][37][38]. Some MYB transcription factors, like SmMYB86, inhibited anthocyanin synthesis by binding to the promoters of Sm-CHS, SmF3H, and SmANS.…”