Study of the efficiency of anticoagulant rodenticides to control<i>Mus musculus domesticus</i>introgressed with<i>Mus spretus Vkorc1</i>

Goulois, Joffrey; Hascoët, Claire; Dorani, Khedidja; Besse, Stéphane; Legros, Lionel; Benoît, Etienne; Lattard, Virginie

doi:10.1002/ps.4319

Cited by 19 publications

(37 citation statements)

References 26 publications

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“…Resistance factors corresponding to the ratio between K i obtained for the mutated VKORC1 and for the wild‐type VKORC1 were determined for each VKORC1 protein toward each anticoagulant rodenticide. These in vitro resistance factors used for the first time to study the consequences of the Vkorc1 mutations in brown rats (Hodroge et al., 2011) were shown to be totally coherent with resistance factors obtained using liver microsomes (Lasseur et al., 2005) and with resistance factors obtained by BCR tests after peros administration of AVK to strains of rats homozygous for wild‐type Vkorc1 or Vkorc1‐Y139F (Grandemange et al., 2009) or by feeding tests in mice homozygous for wild‐type Vkorc1 or Vkorc1 spr (Goulois et al., 2017). Therefore, in this study, the resistance factors determined in vitro using recombinant VKORC1 expressed in yeast are considered as good markers of the in vivo resistance phenotype of rodents, for whom we possessed only fragments of tail.…”

Section: Discussionmentioning

confidence: 99%

Adaptative evolution of theVkorc1gene inMus musculus domesticusis influenced by the selective pressure of anticoagulant rodenticides

Goulois

Lambert

Legros³

et al. 2017

Ecology and Evolution

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Anticoagulant rodenticides are commonly used to control rodent pests worldwide. They specifically inhibit the vitamin K epoxide reductase (VKORC1), which is an enzyme encoded by the Vkorc1 gene, involved in the recycling of vitamin K. Therefore, they prevent blood clotting. Numerous mutations of Vkorc1 gene were reported in rodents, and some are involved in the resistant to rodenticides phenotype. Two hundred and sixty‐six mice tails were received from 65 different locations in France. Coding sequences of Vkorc1 gene were sequenced in order to detect mutations. Consequences of the observed mutations were evaluated by the use of recombinant VKORC1. More than 70% of mice presented Vkorc1 mutations. Among these mice, 80% were homozygous. Contrary to brown rats for which only one predominant Vkorc1 genotype was found in France, nine missense single mutations and four double mutations were observed in house mice. The single mutations lead to resistance to first‐generation antivitamin K (AVKs) only and are certainly associated with the use of these first‐generation molecules by nonprofessionals for the control of mice populations. The double mutations, probably obtained by genetic recombination, lead to in vitro resistance to all AVKs. They must be regarded as an adaptive evolution to the current use of second‐generation AVKs. The intensive use of first‐generation anticoagulants probably allowed the selection of a high diversity of mutations, which makes possible the genetic recombination and consequently provokes the emergence of the more resistant mutated Vkorc1 described to date.

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Section: Discussionmentioning

confidence: 99%

Adaptative evolution of theVkorc1gene inMus musculus domesticusis influenced by the selective pressure of anticoagulant rodenticides

Goulois

Lambert

Legros³

et al. 2017

Ecology and Evolution

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“…A white square indicates that M. m. domesticus has been tested for Vkorc1 mutations related to AR resistance and was found to be negative (this study). A red triangle indicates that M. m. domesticus has been tested for resistance through a lethal feeding period test and was found to be resistant to ARs [5,8,20,25,26]. A white triangle indicates that M. m. domesticus has displayed Vkorc1 mutations related to AR resistance [20].…”

Section: Mus Musculus D-loop Haplotypes In Western Australiamentioning

confidence: 99%

“…These rodents are considered pests, and poisons are the most commonly used method for control and eradication programs [4]. Due to their efficacy, anticoagulant rodenticides (ARs) have been used to carry out eradications on over 300 islands with a 90% success rate [5,6]. Anticoagulant rodenticides can be subdivided into first generation (FGARs) or second generation (SGARs), according to when they were first synthesised and commercialised [7].…”

Section: Introductionmentioning

confidence: 99%

Mus musculus populations in Western Australia lack VKORC1 mutations conferring resistance to first generation anticoagulant rodenticides: Implications for conservation and biosecurity

et al. 2020

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Background Humans routinely attempt to manage pest rodent populations with anticoagulant rodenticides (ARs). We require information on resistance to ARs within rodent populations to have effective eradication programs that minimise exposure in non-target species. Mutations to the VKORC1 gene have been shown to confer resistance in rodents with high proportions of resistance in mice found in all European populations tested. We screened mutations in Mus musculus within Western Australia, by sampling populations from the capital city (Perth) and a remote island (Browse Island). These are the first Australian mouse populations screened for resistance using this method. Additionally, the mitochondrial D-loop of house mice was sequenced to explore population genetic structure, identify the origin of Western Australian mice, and to elucidate whether resistance was linked to certain haplotypes. Results No resistance-related VKORC1 mutations were detected in either house mouse population. A genetic introgression in the intronic sequence of the VKORC1 gene of Browse Island house mouse was detected which is thought to have originated through hybridisation with the Algerian mouse (Mus spretus). Analysis of the mitochondrial D-loop reported two haplotypes in the house mouse population of Perth, and two haplotypes in the population of Browse Island. Conclusions Both house mouse populations exhibited no genetic resistance to ARs, in spite of free use of ARs in Western Australia. Therefore weaker anticoagulant rodenticides can be employed in pest control and eradication attempts, which will result in reduced negative impacts on nontarget species. Biosecurity measures must be in place to avoid introduction of resistant house mice, and new house mouse subspecies to Western Australia.

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“…In Asia, a loss of 5% of total rice production, amounting to 30 million tons (sufficient to feed 180 million individuals for 12 months), has been reported (Singleton 2003). Previous rodent management studies have primarily focused on mechanical controls (Mayamba et al, 2019), chemical rodenticides (Goulois et al, 2017), and biological controls (Mayamba et al 2019;Stenseth et al 2003). However, these prevention control measures can lead to ecological threats of their own (Ettlin & Prentice 2002).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "The reproductive inhibitory effects of levonorgestrel, quinestrol, and EP-1 in Brandt’s vole (Lasiopodomys brandtii) (v0.1)"

2020

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Background. Rodent pests can inflict devastating impacts on agriculture and the environment, leading to significant economic damage associated with their high species diversity, reproductive rates, and adaptability. Fertility control methods can indirectly control rodent pest populations as well as limit ecological consequences and environmental concerns caused by lethal chemical poisons. Brandt's voles, which are common rodent pests that are found in the grasslands of middle-eastern Inner Mongolia, eastern regions of Mongolia, and some regions of southern Russia, were assessed in the present study. Methods. We evaluated the effects of levonorgestrel, quinestrol, and a 1:1 mixture of the two (EP-1) on reproductive behavior as well as changes in the reproductive system, reproductive hormone levels, and toxicity in Brandt's voles. Results. Our results revealed that all three fertility control agents can cause sterility at a dosage of 2 mg/kg. However, quinestrol caused a greater degree of toxicity, as determined by visible liver damage and reduced expression of the detoxifying molecule CYP1A2. Of the remaining two fertility control agents, EP-1 was superior to levonorgestrel in inhibiting the secretion of follicle-stimulating hormone and causing sterility. We believe that these findings will help promote the use of these fertility control agents and, in turn, reduce the use of chemical poisons and limit their detrimental ecological and environmental impacts.

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Study of the efficiency of anticoagulant rodenticides to controlMus musculus domesticusintrogressed withMus spretus Vkorc1

Cited by 19 publications

References 26 publications

Adaptative evolution of theVkorc1gene inMus musculus domesticusis influenced by the selective pressure of anticoagulant rodenticides

Adaptative evolution of theVkorc1gene inMus musculus domesticusis influenced by the selective pressure of anticoagulant rodenticides

Mus musculus populations in Western Australia lack VKORC1 mutations conferring resistance to first generation anticoagulant rodenticides: Implications for conservation and biosecurity

Peer Review #1 of "The reproductive inhibitory effects of levonorgestrel, quinestrol, and EP-1 in Brandt’s vole (Lasiopodomys brandtii) (v0.1)"

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