Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Abstract

Purpose To compare proteomic profiles of spermatozoa from patients with varicocele and poor sperm quality before and after varicocelectomy. Methods This work was designed as a prospective and observational study. The study was based on 20 men with varicocele grade 3 and poor sperm quality undergoing varicocelectomy at the Fertility Unit of Royan institute in 2009. Two semen samples were collected, one before varicocelectomy and the other after surgery. Protein separation was done by two-dimensional protein electrophoresis, and analyzed by gel densitometry and mass spectrometry. Differential sperm protein expression levels were measured by gel densitometry. Results Comparison of the sperm parameters showed that sperm motility and concentration were increased after varicocelectomy. At the level of protein, a total of 3 protein spots were identified whose expression was significantly lower in sperm samples before varicocelectomy compared with after surgery including heat shock protein A5 (HSPA5), superoxide dismutase 1 (SOD1) and δ-subunit of the catalytic core of mitochondrial adenosine triphosphate synthase (ATP5D). Conclusions High grade varicocoele affects sperm protein expression presumably because of increasing testicular temperature. These proteins play essential roles in sperm production, DNA integrity protection, and sperm motility. This novel study demonstrates that varicocelectomy can improve both sperm quality and proteins expression.

Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients

“…It has been shown a relationship between human sperm DNA damage and some semen parameters including concentration, morphology, and motility (Irvine et al, 2000;Lachaud et al, 2004;Henkel et al, 2010;Hosseinifar et al, 2013aHosseinifar et al, , 2014, whereas other studies did not demonstrate such association (Hughes et al, 1996;Henkel et al, 2003;Hosseinifar et al, 2013b). In this study, we also do not detect any significant correlation between DFI and sperm parameters in globozoospermic patients.…”

Effect of varicocelectomy on sperm parameters, oxidative stress and Chromatin maturity in seminal fluid of infertile patients with varicocele

“…The significant decreased SOD1, GSH and CAT levels in seminal plasma of infertile men complaining of varicocele was in agreement with previous study that showed significantly reduced levels of enzymatic antioxidants in the semen of infertile men with varicocele [22]. The level of GSH, SOD1 and CAT had significant increase in varicoceloctomized group compared to varicocele group which in agreement with Hosseinifar et al [23] whom identified SOD1 significantly lower in samples before varicocelectomy compared with after surgery. These proteins are essential for normal sperm production and are affected by the heat generated by the existence of a varicocele.…”

Genome-wide association study for semen traits of the bulls in Chinese Holstein

“…; Hosseinifar et al . ). The DSCAML1 gene, 6 Mb away from the significant SNP BTA‐123344‐no‐rs , was found to increase the occurrence of sperm head defects and to be associated with poor sperm morphology, sperm production and the male reproductive process (Kosova et al .…”