Study of the effect of γ-irradiation on bovine serum samples on the ability of monoclonal antibodies to detect invariant antigens of Trypanosoma congolense, T. vivax and T. brucei in enzyme-linked immunosorbent assays

Rebeski, Dierk E.; Winger, E.M; Aigner, H.; Wright, Peter F.; Crowther, John R.; Dwinger, R.H.

doi:10.1016/s0304-4017(98)00162-9

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…As noted above, gamma irradiation represents an effective method for virus inactivation, but tests may require adjustment, as irradiation can in some circumstances affect their diagnostic sensitivity and specificity. Sera from cattle infected with T. congolense , T. vivax , and T. brucei were irradiated with 30,000 Gy and then used in enzyme-linked immunosorbent assays (ELISAs) for detection of invariant trypanosomal antigens (Rebeski et al 1998). In samples where there were unequivocal positive or negative ELISA results, irradiation had no effect on the diagnostic sensitivity of the assays—all samples shown to be positive before irradiation remained positive and those shown to be negative remained negative.…”

Section: Introductionmentioning

confidence: 99%

The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

Viljoen

Luckins

2012

Trop Anim Health Prod

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Nuclear and nuclear-related technologies have played an important role in animal health, particularly in relation to disease diagnosis and characterization of pathogenic organisms. This review focuses primarily on how and where nuclear technologies, both non-isotopic and isotopic methods, have made their impact in the past and where it might be expected they could have an impact in the future. The review outlines the extensive use of radiation attenuation in attempts to create vaccines for a multiplicity of pathogenic organisms and how the technology is being re-examined in the light of recent advances in irradiation techniques and cryopreservation/lyophilization that might obviate some of the problems of maintenance of viable, attenuate vaccines and their transport and use in the field. This approach could be used for a number of parasitic diseases where vaccination has been problematic and where investigations into the development of molecular vaccines have still failed to deliver satisfactory candidates for generating protective immune responses. Irradiation of antigens or serum samples also has its uses in diagnosis, especially when the samples need to be transported across international boundaries, or when handling the pathogens in question when carrying out a test presents serious health hazards to laboratory personnel. The present-day extensive use of enzyme immunoassays and molecular methods (e.g., polymerase chain reaction) for diagnosis and characterization of animal pathogens has its origins in the use of isotope-labeled antigens and antibodies. These isotopic techniques that included the use of 75Se, 32P, 125I, and 35S isotopes enabled a level of sensitivity and specificity that was hitherto unrealized, and it is prescient to remind ourselves of just how successful these technologies were, in spite of their infrequent use nowadays. Finally, the review looks at the potential for stable isotope analysis for a variety of applications—in the tracking of animal migrations, where the migrant are potential carriers of transboundary animal diseases, and where it would be useful to determine the origins of the carrier, e.g., Highly Pathogenic Avian Influenza and its dissemination by wild water fowl. Other applications could be in monitoring sequestered microbial culture (e.g., rinderpest virus) where in the case of accidental or deliberate release of infective culture it would be possible to identify the laboratory from which the isolate originated.

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Section: Introductionmentioning

confidence: 99%

The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

Viljoen

Luckins

2012

Trop Anim Health Prod

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“…Furthermore, gamma irradiation techniques have been applied for the vaccination and sterilization of vaccines (Creaney et al. 1995; Rebeski et al. 1998; May et al.…”

Section: Introductionmentioning

confidence: 99%

DETECTION OF THE INDIGENOUS SALMONELLA LEVEL IN A RADIATION‐STERILIZED PURE CULTURE AND MEAT MODEL SYSTEM

Kim

Yun

Lee

et al. 2010

Journal of Muscle Foods

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The quantitative detection of the radiation‐sterilized Salmonella typhimurium in a pure culture and ground beef, pork and chicken meat model system was evaluated using a visual immunoprecipitate (VIP) assay kit and an enzyme‐linked immunosorbent assay (ELISA) method. The VIP kit and ELISA detected 105 cells/mL or above of the enriched culture of S. typhimurium, and the detection limit of the irradiated Salmonella was comparable to that of the intact cells. There was no significant difference between the detection limit of the enriched culture and that of inoculated and irradiated S. typhimurium in model system of ground beef, pork and chicken. Irradiation did not give any effect on the immunochemical detection of S. typhimurium within a dose of 5 kGy; therefore, an immunodetection method may be a simple way for the quantitative analysis of the indigenous microbes in a radiation‐sterilized food. PRACTICAL APPLICATIONS One of the consumers' concerns about food irradiation technology is the abuse of the technology to sterilize food products without meeting the hygienic standard such as good manufacturing practice before irradiation of food. A simple method to detect the indigenous microorganism level before irradiation process is tested as a quality assurance measure using a pure culture of Salmonella typhimurium and inoculated meat model system. This simple test will be helpful by providing a trust for both consumer and manufacturer to verify that their products are produced under hygienic standard before irradiation treatment.

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Effect of γ-irradiation on serum samples on the diagnostic performance of ELISA methods for the detection of trypanosomal antibodies

Rebeski

Winger

Gabler

et al. 2001

Veterinary Parasitology

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Study of the effect of γ-irradiation on bovine serum samples on the ability of monoclonal antibodies to detect invariant antigens of Trypanosoma congolense, T. vivax and T. brucei in enzyme-linked immunosorbent assays

Cited by 4 publications

References 18 publications

The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

DETECTION OF THE INDIGENOUS SALMONELLA LEVEL IN A RADIATION‐STERILIZED PURE CULTURE AND MEAT MODEL SYSTEM

Effect of γ-irradiation on serum samples on the diagnostic performance of ELISA methods for the detection of trypanosomal antibodies

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