Study of Serotypes, Susceptibility to Macrolide and Virulence and Resistance Molecular Profiles in Invasive Strains of &amp;lt;i&amp;gt;Streptococcus agalactiae&amp;lt;/i&amp;gt; in Two Argentine Provinces

Laczeski, Margarita Ester; Novosak, Marina Gisel; Giolito, Roxana Cannistraci; Littvik, Ana; Pavan, Jorge Victorio; Villalba, Viviana; Vergara, Marta

doi:10.4236/aim.2015.54021

Cited by 2 publications

(1 citation statement)

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“…In a study carried out with strains that cause invasive diseases in the province of Misiones, all genes were detected in 72.73% of the isolates and it is observed that the strains recovered from neonates present all the virulence factors in percentages greater than 81.80%. However, the number of isolates analyzed was lower (n = 11) despite belonging to all invasive isolates recovered simultaneously during the years in which the present study was conducted [62].…”

Section: Molecular Detection Of Virulence-associated Genesmentioning

confidence: 77%

Prevalence, serotypes and virulence genes of Streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation

et al. 2021

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Background In pregnant women Streptococcus agalactiae (GBS) can be transmitted to newborn causing severe infections. It is classified into 10 serotypes (Ia, Ib, II-IX). The severity of neonatal disease is determined by the capsular serotype and virulence factors such as the polysaccharide capsule, encoded by the cps gene, protein C, which includes the Cα surface proteins (bca gene), Rib (rib gene) and Cβ (bac gene); the proteins Lmb (lmb gene), FbsB (fbsB gene), FbsA (fbsA gene), the cyl operon encoding a β-hemolysin (hylB gene), the CAMP factor (cfb gene) and the C5a peptidase (scpB gene). The aim of this work was to determine the degree of GBS colonization in pregnant women, the serotypes distribution and to investigate virulence-associated genes. Methods We worked with 3480 samples of vagino-rectal swabs of women with 35–37 weeks of gestation. The identification of the strains was carried out using conventional biochemical tests and group confirmatory serology using a commercial latex particle agglutination kit. Two hundred GBS strains were selected. Their serotype was determined by agglutination tests. The monoplex PCR technique was used to investigate nine virulence-associated genes (cps, bca, rib, bac, lmb, fbsB, fbsA, hylB and scpB). Results The maternal colonization was 9.09%. The serotypes found were: Ia (33.50%), III (19.00%), Ib (15.50%), II (14.00%), V (7.00%) and IX (5.50%). 5.50% of strains were found to be non-serotypeable (NT). The nine virulence genes investigated were detected simultaneously in 36.50% of the strains. The genes that were most frequently detected were scpB (100.00%), fbsA (100.00%), fbsB (100.00%), cylB (95.00%), lmb (94.00%) and bca (87.50%). We found associations between serotype and genes bac (p = 0.003), cylB (p = 0.02), rib (p = 0.01) and lmb (p < 0.001). Conclusions The frequency of vaginal-rectal colonization, serotypes distribution and associated virulence genes, varies widely among geographical areas. Therefore, epidemiological surveillance is necessary to provide data to guide decision-making and planning of prevention and control strategies.

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Section: Molecular Detection Of Virulence-associated Genesmentioning

confidence: 77%