Study of robustness of filamentous bacteriophages for industrial applications

Branston, Steven; Stanley, Emma; Ward, John M.; Keshavarz-Moore, E

doi:10.1002/bit.23066

Cited by 21 publications

(6 citation statements)

References 16 publications

(30 reference statements)

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“…It was thought that the very long but thin shape of M13 and other filamentous phages would increase their shear sensitivity in the various kinds of industrialscale processing equipment of pumps, continuous centrifuges and membrane filters. This was seen not to be the case [18] which is highly advantageous for largescale downstream processing of this and other filamentous phages. Filamentous phages have a rather special property in that they do not lyse their host, but set up a permanently infected state, and new, progeny phage is extruded through special structures in the cell wall.…”

Section: M13: a Filamentous Bacteriophagementioning

confidence: 87%

Scale-Up and Bioprocessing of Phages

Ward¹,

Branston²,

Stanley³

et al. 2020

Bacteriophages - Perspectives and Future

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A profusion of new applications for phage technologies has been developed within the last few years, stimulating investigations into the large-scale production of different phages. Applications such as antibiotic replacement, phages as gene therapy vectors, phages as vaccines, diagnostics using filamentous phages and novel optical applications such as the phage laser may need grams to kilogrammes of phage in the future. However, many of the techniques that are used for the growth and purification of bacteriophage at small scale are not transferable to large-scale production facilities of phage in industrial processes. In this chapter, the stages of production that need to be carried out at scale are examined for the efficient large-scale fermentation of the filamentous phage M13 and the Siphoviridae phage lambda (λ). A number of parameters are discussed: the multiplicity of infection (MOI) of phage to host cells, the impact of agitation on the initial infection stages, the co-growth with phage rather than static attachment, the use of engineered host cells expressing nuclease, the optimisation of both the quantity and the physiology of the E. coli inoculum and phage precipitation methods.

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Section: M13: a Filamentous Bacteriophagementioning

confidence: 87%

Scale-Up and Bioprocessing of Phages

Ward¹,

Branston²,

Stanley³

et al. 2020

Bacteriophages - Perspectives and Future

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“…Ff is very stable, resisting exposure to pH extremes, detergents, and heat (Branston et al 2011(Branston et al , 2013. Given that phage display library screening by biopanning typically requires detergents and low pH at the wash and elution steps, respectively, these properties are precious, and they place the Ff as preferred particles for phage display, ahead of tailed phages like λ, which are sensitive to low pH (see Overview: Principles of Affinity Selection [Smith 2023]).…”

Section: Physical Properties Of the Ff Filamentous Phagesmentioning

confidence: 99%

Structure, Biology, and Applications of Filamentous Bacteriophages

Rakonjac

Gold

León-Quezada

et al. 2023

Cold Spring Harb Protoc

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The closely relatedEscherichia coliFf filamentous phages (f1, fd, and M13) have taken a fantastic journey over the past 60 years, from the urban sewerage from which they were first isolated, to their use in high-end technologies in multiple fields. Their relatively small genome size, high titers, and the virions that tolerate fusion proteins make the Ffs an ideal system for phage display. Folding of the fusions in the oxidizing environment of theE. coliperiplasm makes the Ff phages a platform that allows display of eukaryotic surface and secreted proteins, including antibodies. Resistance of the Ffs to a broad range of pH and detergents facilitates affinity screening in phage display, whereas the stability of the virions at ambient temperature makes them suitable for applications in material science and nanotechnology. Among filamentous phages, only the Ffs have been used in phage display technology, because of the most advanced state of knowledge about their biology and the various tools developed forE. colias a cloning host for them. Filamentous phages have been thought to be a rather small group, infecting mostly Gram-negative bacteria. A recent discovery of more than 10 thousand diverse filamentous phages in bacteria and archaea, however, opens a fascinating prospect for novel applications. The main aim of this review is to give detailed biological and structural information to researchers embarking on phage display projects. The secondary aim is to discuss the yet-unresolved puzzles, as well as recent developments in filamentous phage biology, from a viewpoint of their impact on current and future applications.

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“…Large‐scale utilization of bacteriophage in material science, pharmaceutical applications or as ssDNA scaffold for DNA origami requires investigation to enable industrial usage. Progress was achieved by Branston et al () concerning the robustness of filamentous bacteriophage M13 for large‐scale processing. Recently, a new technology was developed for endotoxin removal from bacteriophages for pharmaceutical applications (Branston et al, ).…”

Section: Introductionmentioning

confidence: 99%

Specific growth rate and multiplicity of infection affect high‐cell‐density fermentation with bacteriophage M13 for ssDNA production

Kick

Hensler

Praetorius

et al. 2016

Biotech & Bioengineering

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The bacteriophage M13 has found frequent applications in nanobiotechnology due to its chemically and genetically tunable protein surface and its ability to self-assemble into colloidal membranes. Additionally, its single-stranded (ss) genome is commonly used as scaffold for DNA origami. Despite the manifold uses of M13, upstream production methods for phage and scaffold ssDNA are underexamined with respect to future industrial usage. Here, the high-cell-density phage production with Escherichia coli as host organism was studied in respect of medium composition, infection time, multiplicity of infection, and specific growth rate. The specific growth rate and the multiplicity of infection were identified as the crucial state variables that influence phage amplification rate on one hand and the concentration of produced ssDNA on the other hand. Using a growth rate of 0.15 h and a multiplicity of infection of 0.05 pfu cfu in the fed-batch production process, the concentration of pure isolated M13 ssDNA usable for scaffolded DNA origami could be enhanced by 54% to 590 mg L . Thus, our results help enabling M13 production for industrial uses in nanobiotechnology. Biotechnol. Bioeng. 2017;114: 777-784. © 2016 Wiley Periodicals, Inc.

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Study of robustness of filamentous bacteriophages for industrial applications

Cited by 21 publications

References 16 publications

Scale-Up and Bioprocessing of Phages

Scale-Up and Bioprocessing of Phages

Structure, Biology, and Applications of Filamentous Bacteriophages

Specific growth rate and multiplicity of infection affect high‐cell‐density fermentation with bacteriophage M13 for ssDNA production

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