Study of Recellularized Human Acellular Arterial Matrix Repairs Porcine Biliary Segmental Defects

Abstract

BACKGROUND: With the popularity of laparoscopic cholecystectomy, common bile duct injury has been reported more frequently. There is no perfect method for repairing porcine biliary segmental defects. METHODS: After the decellularization of human arterial blood vessels, the cells were cultured with GFP ? (carry green fluorescent protein) porcine bile duct epithelial cells. The growth and proliferation of porcine bile duct epithelial cells on the human acellular arterial matrix (HAAM) were observed by hematoxylin-eosin (HE) staining, electron microscopy, and immunofluorescence. Then, the recellularized human acellular arterial matrix (RHAAM) was used to repair biliary segmental defects in the pig. The feasibility of it was detected by magnetic resonance cholangiopancreatography, liver function and blood routine changes, HE staining, immunofluorescence, real-time quantitative PCR (RT-qPCR), and western blot. RESULTS: After 4 weeks (w) of co-culture of HAAM and GFP ? porcine bile duct epithelial cells, GFP ? porcine bile duct epithelial cells grew stably, proliferated, and fused on HAAM. Bile was successfully drained into the duodenum without bile leakage or biliary obstruction. Immunofluorescence detection showed that GFP-positive bile duct cells could still be detected after GFP-containing bile duct cells were implanted into the acellular arterial matrix for 8 w. The implanted bile duct cells can successfully resist bile invasion and protect the acellular arterial matrix until the newborn bile duct is formed. CONCLUSION: The RHAAM can be used to repair biliary segmental defects in pigs, which provides a new idea for the clinical treatment of common bile duct injury.

The analgesic effects of β-elemene in rats with neuropathic pain by inhibition of spinal astrocytic ERK activation

“…The lumbar 4-6 segments of the spinal cord were harvested according to the termination of dorsal roots, postfixed in 10% PFA solution for 48 h, embedded in paraffin wax (Shanghai Huayong Olefin Co., Ltd, Shanghai, China), and then serially cut into 5 μm thick sections on a microtome (Leica RM2016, Shanghai, China). All sections were divided into nine series, which were subsequently subjected to double immunofluorescence staining according to a previous study 36 for p-ERK and NeuN, p-ERK and glial fibrillary acidic protein (GFAP), p-ERK and ionized calcium-binding adaptor molecule (Iba-1), p-JNK and NeuN, p-JNK and GFAP, p-JNK and Iba-1, p-p38 and NeuN, p-p38 and GFAP, and p-p38 and Iba-1.…”