Study of proteins associated with the Eimeria tenella refractile body by a proteomic approach

Venevelles, Patrick de; Chich, Jean François; Faigle, Wolfgang; Lombard, Bérengère; Loew, Damarys; Pery, P.; Labbé, M.

doi:10.1016/j.ijpara.2006.06.018

Cited by 30 publications

(22 citation statements)

References 26 publications

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“…Therefore, we also used the Eimeria antisera to screen the immunodominant antigens of Eimeria species and obtained 85 immunodominant proteins. Part of the identified antigens were also identified as immunodominant antigens in previous studies, including MIC3, pyruvate kinase, enolase, actin, aspartyl proteinase, 14-3-3 protein, lactate dehydrogenase and so on [29–31]. Some of the identified antigens have been demonstrated to be able to confer protection against Eimeria challenge, such as lactate dehydrogenase [32], microneme 2 [33], microneme 7 [34] and so on.…”

Section: Discussionmentioning

confidence: 99%

Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis

et al. 2017

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Clinical chicken coccidiosis is mostly caused by simultaneous infection of several Eimeria species, and host immunity against Eimeria is species-specific. It is urgent to identify common immunodominant antigen of Eimeria for developing multivalent anticoccidial vaccines. In this study, sporozoite proteins of Eimeria tenella, Eimeria acervulina and Eimeria maxima were analyzed by two-dimensional electrophoresis (2DE). Western bot analysis was performed on the yielded 2DE gel using antisera of E. tenella E. acervulina and E. maxima respectively. Next, the detected immunodominant spots were identified by comparing the data from MALDI-TOF-MS/MS with available databases. Finally, Eimeria common antigens were identified by comparing amino acid sequence between the three Eimeria species. The results showed that analysis by 2DE of sporozoite proteins detected 629, 626 and 632 protein spots from E. tenella, E. acervulina and E. maxima respectively. Western bot analysis revealed 50 (E. tenella), 64 (E. acervulina) and 57 (E. maxima) immunodominant spots from the sporozoite 2DE gels of the three Eimeria species. The immunodominant spots were identified as 33, 27 and 25 immunodominant antigens of E. tenella, E. acervulina and E. maxima respectively. Fifty-four immunodominant proteins were identified as 18 ortholog proteins among the three Eimeria species. Finally, 5 of the 18 ortholog proteins were identified as common immunodominant antigens including elongation factor 2 (EF-2), 14-3-3 protein, ubiquitin-conjugating enzyme domain-containing protein (UCE) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In conclusion, our results not only provide Eimeria sporozoite immunodominant antigen map and additional immunodominant antigens, but also common immunodominant antigens for developing multivalent anticoccidial vaccines.

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Section: Discussionmentioning

confidence: 99%

Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis

et al. 2017

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“…The multiple crystalloid bodies in ookinetes, earlier thought to be virus-like particles, have recently been shown to contain important proteins such as the LCCL family proteins involved in midgut invasion in P. berghei. 51 Recently, the proteome of crystalloid bodies in Eimeria tenella, an apicomplexan protozoan that causes chicken coccidiosis, was analyzed and confirmed as a reservoir of proteins necessary for invasion and energetic and metabolic functions, 52 but such findings needs to be corroborated and extended in Plasmodium. Recent proteomic studies of Plasmodium zygote and ookinete stage proteins 53,54 will facilitate functional analysis of these sexual stage proteins by use of improved electron microscopic techniques.…”

Section: Discussionmentioning

confidence: 99%

New Ultrastructural Analysis of the Invasive Apparatus of the Plasmodium Ookinete

Patra¹,

Vinetz²

2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Invasion of the mosquito midgut by the Plasmodium ookinete determines the success of transmission of malaria parasites from humans to mosquitoes and therefore, is a potential target for molecular intervention. Here, we show higher-resolution ultrastructural details of developing and mature P. gallinaceum ookinetes than previously available. Improved fixation and processing methods yielded substantially improved transmission electron micrographs of ookinetes, particularly with regard to visualization of subcellular secretory and other organelles. These new images provide new insights into the synthesis and function of vital invasive machinery focused on the following features: apical membrane protrusions presumptively used for attachment and protein secretion, dark spherical bodies at the apical end of the mature ookinete, and the presence of a dense array of micronemes apposed to microtubules at the apical end of the ookinete involved in constitutive secretion. This work advances understanding of the molecular and cellular details of the Plasmodium ookinete and provides the basis of future, more detailed mechanistic experimentation on the biology of the Plasmodium ookinete.

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“…In maturing and mature macromeronts, the staining was concentrated to small spherical peripheral bodies. Refractile bodies are prominent organelles in sporozoites of Eimeriidae and proteome analysis confirmed that these organelles are reservoirs for proteins probably involved in host cell invasion (de Venevelles et al 2006). During merogony, refractile bodies of Eimeria spp.…”

Section: Discussionmentioning

confidence: 84%

“…The dye binds to aminomoieties (McCabe et al 2002) and therefore should predominantly react with basic proteins, particularly to those with many moieties, e. g. histones, hyaluronates, glycosaminglycans or amino sugars (Suniara et al 2000;Spadaro et al 2007). However, recent proteomic studies on refractile bodies of E. tenella (de Venevelles et al 2006) rather suggest a predominance of acidic proteins than basic ones and to our knowledge other studies on the composition of refractile bodies are not available.…”

Section: Discussionmentioning

confidence: 91%

Fluorescent Eimeria bovis sporozoites and meront stages in vitro: a helpful tool to study parasite–host cell interactions

et al. 2008

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A fluorescence-based technique was established to trace intracellular sporozoites of Eimeria bovis for tests on gliding motility, invasion, replication and quantification of infection rates in cultured bovine umbilical vein endothelial cells (BUVEC) by laser scanning confocal microscopy and flow cytometry (FCM) analyses. Employing the fluorescent dye 5(6)-carboxyfluorescein diacetate succinimidyl ester (CFSE), we determined its effects on sporozoites at various concentrations and duration of staining. More than 98% of sporozoites were labelled with the dye at a concentration of 2.5 muM. Staining was predominantly found in refractile bodies and presumptive micronemes. Upon infection of BUVEC, CFSE-labelled sporozoites developed into fluorescent immature macromeronts, which were traceable inside the cells until 22 days postinfection (p. i.). Consistent with a peripheral localisation of the fluorescence signal in macromeronts merozoites released from these lacked detectable fluorescence. As example of use, a multicolour FCM approach for the simultaneous determination of E. bovis infection and host cell surface molecule expression was established. The approach proved suitable to quantify major histocompatibility complex (MHC-I) and MHC-II expression, thereby clearly distinguishing between infected and uninfected BUVEC up to day 14 p. i. In conclusion, CFSE labelling of E. bovis sporozoites facilitates monitoring of intracellular stages in vitro and will be a highly useful tool for studying host cell responses towards parasite invasion.

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Study of proteins associated with the Eimeria tenella refractile body by a proteomic approach

Cited by 30 publications

References 26 publications

Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis

Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis

New Ultrastructural Analysis of the Invasive Apparatus of the Plasmodium Ookinete

Fluorescent Eimeria bovis sporozoites and meront stages in vitro: a helpful tool to study parasite–host cell interactions

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