2016
DOI: 10.18632/aging.100950
View full text |Buy / Rent full text
|
Sign up to set email alerts
|

Abstract: Reprogramming of somatic cells into a pluripotent state is known to be accompanied by extensive restructuring of mitochondria and switch in metabolic requirements. Here we utilized Leber's hereditary optic neuropathy (LHON) as a mitochondrial disease model to study the effects of homoplasmic mtDNA mutations and subsequent oxidative phosphorylation (OXPHOS) defects in reprogramming. We obtained fibroblasts from a total of 6 LHON patients and control subjects, and showed a significant defect in complex I respira… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
27
0

Year Published

2016
2016
2020
2020

Publication Types

Select...
3
1

Relationship

0
4

Authors

Journals

citations
Cited by 35 publications
(27 citation statements)
references
References 43 publications
(54 reference statements)
0
27
0
Order By: Relevance
“…A high respiratory capacity is crucial for high malignant and invasive potential 45 . In contrast to tumors, human skeletal muscle and fibroblasts heavily rely on N-pathway capacity 46,47 and a similar compensatory mechanism is not known for mt-myopathies. Ultimately, our results suggest that the immediate net effect of deleterious mtDNA mutations is compensated and does not hamper cellular ATP homeostasis in primary human PCa.…”
Section: Discussionmentioning
confidence: 99%
“…A high respiratory capacity is crucial for high malignant and invasive potential 45 . In contrast to tumors, human skeletal muscle and fibroblasts heavily rely on N-pathway capacity 46,47 and a similar compensatory mechanism is not known for mt-myopathies. Ultimately, our results suggest that the immediate net effect of deleterious mtDNA mutations is compensated and does not hamper cellular ATP homeostasis in primary human PCa.…”
Section: Discussionmentioning
confidence: 99%
“…1C and Supplemental Fig S2B). A recent study reported that the process involved in reprogramming is modulated through telomerase activity, 31 restructuring of mitochondria 32 or histone post-translational modification. 33 Among them, chromatin remodeling has emerged as a major mediator of non-targeted effects associated with radiation and pluripotency reprogramming.…”
Section: Discussionmentioning
confidence: 99%
“…More specifically, two BCCs with highly ordered surface topography were selected from our library1721. Due to the simplicity of the process, this fabrication process has the potential for large-scale manufacturing.…”
Section: Discussionmentioning
confidence: 99%
“…The isolated clonal hiPSCs were maintained on mitotically inactivated mouse embryonic fibroblasts feeders in the presence of DMEM/F-12 medium containing 1x GlutaMAX, 20% knockout serum replacement, 10 ng/ml basic fibroblast growth factor, 0.1 mM non-essential amino acids, 100 μM β-mercaptoethanol and 0.5x (50 U/mL) penicillin/streptomycin (all from Invitrogen). Further characterization of hiPSCs was performed to assess the expression of pluripotent markers OCT4 and TRA-1-60, as described previously21. Briefly, immunocytochemistry was performed with antibodies against OCT4 (#sc-5279, Santa Cruz Biotechnology) or TRA-1-60 (#AB16288, Abcam), followed by the appropriate Alexa-fluor 488 and DAPI counterstaining (Invitrogen).…”
Section: Methodsmentioning
confidence: 99%