Study of microRNA expression in Salmonella Typhimurium-infected porcine ileum reveals miR-194a-5p as an important regulator of the TLR4-mediated inflammatory response

Herrera-Uribe, Júber; Zaldívar‐López, Sara; Aguilar, Carmen; Entrenas-García, Carmen; Bautista, Rocí­o; Claros, Manuel Gonzalo; Garrido, Juan J.

doi:10.1186/s13567-022-01056-7

Cited by 6 publications

(2 citation statements)

References 61 publications

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“…A study by Huang et al ( 143 ), explored the peripheral blood miRNA expression profile after Salmonella enterica serovar Typhimurium infection revealed that 29 miRNAs had different expressions. In addition, studies with miR-146a induction on the fecal bacterial load in pigs suggest its potential role in modulating the fecal bacterial load in pigs during Salmonella infection ( 144 ). Further, the functional analysis of porcine miR-194a-5p, analogous to human miR-194-5p, revealed its significant role in regulating the expression of the TLR4 gene.…”

Section: Mirnas Involved In Swine Diseasesmentioning

confidence: 99%

MicroRNAs: exploring their role in farm animal disease and mycotoxin challenges

Kappari,

Dasireddy,

Applegate

et al. 2024

Front. Vet. Sci.

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MicroRNAs (miRNAs) serve as key regulators in gene expression and play a crucial role in immune responses, holding a significant promise for diagnosing and managing diseases in farm animals. This review article summarizes current research on the role of miRNAs in various farm animal diseases and mycotoxicosis, highlighting their potential as biomarkers and using them for mitigation strategies. Through an extensive literature review, we focused on the impact of miRNAs in the pathogenesis of several farm animal diseases, including viral and bacterial infections and mycotoxicosis. They regulate gene expression by inducing mRNA deadenylation, decay, or translational inhibition, significantly impacting cellular processes and protein synthesis. The research revealed specific miRNAs associated with the diseases; for instance, gga-miR-M4 is crucial in Marek’s disease, and gga-miR-375 tumor-suppressing function in Avian Leukosis. In swine disease such as Porcine Respiratory and Reproductive Syndrome (PRRS) and swine influenza, miRNAs like miR-155 and miR-21-3p emerged as key regulatory factors. Additionally, our review highlighted the interaction between miRNAs and mycotoxins, suggesting miRNAs can be used as a biomarker for mycotoxin exposure. For example, alterations in miRNA expression, such as the dysregulation observed in response to Aflatoxin B1 (AFB1) in chickens, may indicate potential mechanisms for toxin-induced changes in lipid metabolism leading to liver damage. Our findings highlight miRNAs potential for early disease detection and intervention in farm animal disease management, potentially reducing significant economic losses in agriculture. With only a fraction of miRNAs functionally characterized in farm animals, this review underlines more focused research on specific miRNAs altered in distinct diseases, using advanced technologies like CRISPR-Cas9 screening, single-cell sequencing, and integrated multi-omics approaches. Identifying specific miRNA targets offers a novel pathway for early disease detection and the development of mitigation strategies against mycotoxin exposure in farm animals.

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Section: Mirnas Involved In Swine Diseasesmentioning

confidence: 99%

MicroRNAs: exploring their role in farm animal disease and mycotoxin challenges

Kappari,

Dasireddy,

Applegate

et al. 2024

Front. Vet. Sci.

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“…miRNAs are noncoding RNA molecules of roughly 19 to 25 nt in length, and they can regulate the expression levels of gene by binding to specific messenger RNA (mRNA) sequences [ 17 , 18 ]. In domestic animals, miRNAs have been reported to play a pivotal part in regulating skeletal muscle and fat development as well as immune response [ 19 , 20 , 21 ]. miRNAs are key regulators of FE.…”

Section: Introductionmentioning

confidence: 99%

Identification of Differentially Expressed miRNAs in Porcine Adipose Tissues and Evaluation of Their Effects on Feed Efficiency

Liao

Ren

Miao

2022

Genes

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Feed efficiency (FE) is a very important trait affecting the economic benefits of pig breeding enterprises. Adipose tissue can modulate a variety of processes such as feed intake, energy metabolism and systemic physiological processes. However, the mechanism by which microRNAs (miRNAs) in adipose tissues regulate FE remains largely unknown. Therefore, this study aimed to screen potential miRNAs related to FE through miRNA sequencing. The miRNA profiles in porcine adipose tissues were obtained and 14 miRNAs were identified differentially expressed in adipose tissues of pigs with extreme differences in FE, of which 9 were down-regulated and 5 were up-regulated. GO and KEGG analyses indicated that these miRNAs were significantly related to lipid metabolism and these miRNAs modulated FE by regulating lipid metabolism. Subsequently, quantitative reverse transcription–polymerase chain reaction (qRT-PCR) of five randomly selected DEMs was used to verify the reliability of miRNA-seq data. Furthermore, 39 differentially expressed target genes of these DEMs were obtained, and DEMs–target mRNA interaction networks were constructed. In addition, the most significantly down-regulated miRNAs, ssc-miR-122-5p and ssc-miR-192, might be the key miRNAs for FE. Our results reveal the mechanism by which adipose miRNAs regulate feed efficiency in pigs. This study provides a theoretical basis for the further study of swine feed efficiency improvement.

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Predictive action of oncomiR in suppressing TP53 signaling pathway in hypoxia‐conditioned colon cancer cell line HCT‐116

Susanti,

Dafip,

Mustikaningtyas

et al. 2024

Cell Biology International

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Hypoxia‐induced heterogeneity in colorectal cancer (CRC) significantly impacts patient survival by promoting chemoresistance. These conditions alter the regulation of miRNAs, key regulators of crucial processes like proliferation, apoptosis, and invasion, leading to tumor progression. Despite their promising potential as diagnostic and therapeutic targets, the underlying mechanisms by which miRNAs influence hypoxia‐mediated tumorigenesis remain largely unexplored. This study aims to elucidate the action of miRNAs in HCT‐116 colorectal cancer stem cells (CSCs) under hypoxia, providing valuable insights into their role in tumor adaptation and progression. MiRNA expression was determined using Nanostring nCounter, and bioinformatic analysis was performed to explain the molecular pathway. A total of 50 miRNAs were obtained with an average count of ≥ 20 reads for comparative expression analysis. The results showed that hypoxia‐affected 36 oncomiRs were increased in HCT‐116, and 14 suppressor‐miRs were increased in MSCs. The increase in miRNA expression occurred consistently from normoxia to hypoxia and significantly differed between mesenchymal stem cells (MSCs) and HCT‐116. Furthermore, miR‐16‐5p and miR‐29a‐3p were dominant in regulating the p53 signaling pathway, which is thought to be related to the escape mechanism against hypoxia and maintaining cell proliferation. More research with a genome‐transcriptome axis approach is needed to fully understand miRNAs’ role in adapting CRC cells and MSCs to hypoxia. Further research could focus on developing specific biomarkers for diagnosis. In addition, anti‐miR can be developed as a therapy to prevent cancer proliferation or inhibit the adaptation of cancer cells to hypoxia.

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Study of microRNA expression in Salmonella Typhimurium-infected porcine ileum reveals miR-194a-5p as an important regulator of the TLR4-mediated inflammatory response

Cited by 6 publications

References 61 publications

MicroRNAs: exploring their role in farm animal disease and mycotoxin challenges

MicroRNAs: exploring their role in farm animal disease and mycotoxin challenges

Identification of Differentially Expressed miRNAs in Porcine Adipose Tissues and Evaluation of Their Effects on Feed Efficiency

Predictive action of oncomiR in suppressing TP53 signaling pathway in hypoxia‐conditioned colon cancer cell line HCT‐116

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