Study of ion suppression for phenolic compounds in medicinal plant extracts using liquid chromatography–electrospray tandem mass spectrometry

Faccin, Henrique; Viana, Carine; Nascimento, Paulo Cı́cero do; Bohrer, Denise; Carvalho, Leandro Machado de

doi:10.1016/j.chroma.2015.12.017

Cited by 35 publications

(24 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the use of ultra-high-performance LC (UHPLC) allows an increase in the separation efficiency, while the analysis times can be considerably reduced. Additionally, tandem mass spectrometry using triple quadrupole analyzers is a powerful quantification tool (Faccin et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…The phenolic compounds were determined by the method previously developed by Faccin et al (2016). This method used a gradient elution composed of 0.1% acetic acid in water (A) and acetonitrile (B) as the mobile phase at a constant flow rate (0.8 mL min -1 ) according to the following elution program: 8.0% B (0.00-0.10 min); 8.0-25.8% B (0.10-3.45 min); 25.8-54.0% B (3.45-6.90 min); 54.0-100.0% B (6.90-7.00 min); and 100.0% B (7.00-9.00 min).…”

Section: Chromatographic Analysis Of Phenolic Compoundsmentioning

confidence: 99%

See 1 more Smart Citation

Determination of phenolic and triterpenic compounds in Jatropha gossypiifolia L by Ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS)

Silveira

Leal

Molin

et al. 2020

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

This work describes the chemical characterization of extracts of Jatropha gossypiifolia (from Amazonia region) concerning the presence of phenolic and triterpenic compounds using Ultra High Perfomance Liquid chromatography coupled to Mass Spectrometry in Tandem (UHPLC-MS/MS) methods. The studied compounds belong to the most frequently found classes in medicinal plants (triterpenes, flavonoids, flavones, hydroxycinnamic acids, coumarins, catechins and stilbenes), which have been analyzed as chemical and bioactive markers in hydroethanolic and aqueous extracts. Several polyphenolic compounds identified herein are unprecedented in the scientific literature for this species. The chemical markers identified and quantified in the studied extracts of J. gossypiifolia were gallic acid, chlorogenic acid, catechin, caffeic acid, vanillic acid, p-coumaric acid, ferullic acid, rutin, quercitrin, 3-acetylcoumarin, trans-cinnamic acid, quercetin, luteolin, apigenin, kaempferol, chrysin. α-amyrin, β-amyrin and lupeol. As the majority compounds, (+)-catechin, p-coumaric acid, ferulic acid, luteolin, α-amyrin and β-amyrin were found to be present at mg kg −1 levels. J. gossypiifolia extracts presented a high in vitro activity against different reactive oxygen species (hydroxyl, peroxyl, and superoxide anion radicals). Several polyphenolic compound data presented herein are unprecedented in the scientific literature for this plant species. As a result, this plant can be a new source of bioactive molecules for therapeutic purposes.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Chromatographic Analysis Of Phenolic Compoundsmentioning

confidence: 99%

Determination of phenolic and triterpenic compounds in Jatropha gossypiifolia L by Ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS)

Silveira

Leal

Molin

et al. 2020

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

show abstract

“…Just prior to the chromatographic analysis, the eluate obtained by the SPE procedure was diluted to 0.5 g L -1 with 0.1% (1:1; v/v) methanol/acetic acid solution. 22…”

Section: Preparation Of Solid-phase Extraction (Spe)mentioning

confidence: 99%

Antimicrobial, Cyto and Genotoxic Activities of Equisetum hyemale

Alves¹,

Bonez²,

Ebling³

et al. 2019

View full text Add to dashboard Cite

The popular use of natural products has great benefits for the health of the human population. In this study we highlight Equisetum hyemale, belonging to the phylum Sphenophyta and the family Equisetaceae. Popularly, the stems of this plant are used for their diuretic, digestive, anti-anemic, and anti-inflammatory properties. Given this context, and the fact that the growth of antimicrobial resistance is a serious problem for global public health, this plant could be used as an alternative, to increase our therapeutic arsenal. Therefore, it is important to more clearly elucidate the complex structures present in plants, because these substances are mainly responsible for their beneficial and/or toxic effects. Thus, the objective of this study was evaluate the antimicrobial activity, cytotoxic and genotoxic potential of the phenolic compounds separately. The substances luteolin, ferulic acid and coumarin were identified at high concentrations by ultra-high performance chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS). Antimicrobial activity was elucidated through microdilution in broth; the phenolic compounds were able to inhibit the visible growth of the standard bacterial strains at low concentrations. Cytotoxicity was evaluated by MTT and genotoxicity was analysed through cellular damage using a Comet assay; the results showed that it did not present cytoxicity or genotoxicity at the corresponding concentrations. With this, we suggest that E. hyemale may be an alternative for the treatment of infections by microorganisms that are resistant to synthetic drugs. . Advisor (Master and Doctorate) in the Graduate Program in Pharmaceutical Sciences of UFSM and PPG-Nanosciences of UNIFRA.

show abstract

“…More recently, it has been verified that these analytical techniques are quite susceptible to interferences from the sample matrix, which may affect reproducibility, accuracy, selectivity and sensitivity . At this point, it is important to distinguish between matrix effects and interferences.…”

Section: Ion Suppressionmentioning

confidence: 99%

“…Therefore hinders an accurate quantification or, in other words, precision . Moreover, ion suppression does not solely affect the target compound but also the internal standard hence leading to an erroneous estimation of the analyte concentration and increasing the risk of false positive results . It must be noted that as these changes are affected by the concentration of the endogenous/exogenous agent, the response may not be linear.…”

Section: Ion Suppressionmentioning

confidence: 99%

Suitable simple and fast methods for selective isolation of phospholipids as a tool for their analysis

et al. 2018

View full text Add to dashboard Cite

Lipids are gaining relevance over the last 20 years, as our knowledge about their role has changed from merely energy/structural molecules to compounds also involved in several biological processes. This led to the creation in 2003 of a new emerging research field: lipidomics. In particular the phospholipids have pharmacological/food applications, participate in cell signalling/homeostatic pathways while their analysis faces some challenges. Their fractionation/purification is, in fact, especially difficult, as they are amphiphilic compounds. Moreover, it usually involves SPE or TLC procedures requiring specific materials hampering their suitableness for routine analysis. Finally, they can interfere with the ionization of other molecules during mass spectrometry analysis. Thus, simple high-throughput reliable methods to selectively isolate these compounds based on the difference between chemical characteristics of lipids would represent valuable tools for their study besides that of other compounds. The current review work aims to describe the state-of-the-art related to the extraction of phospholipids using liquid-liquid methods for their targeted isolation. The technological and biological importance of these compounds and ion suppression phenomena are also reviewed. Methods by precipitation with acetone or isolation using methanol seem to be suitable for selective isolation of phospholipids in both biological and food samples.

show abstract

Study of ion suppression for phenolic compounds in medicinal plant extracts using liquid chromatography–electrospray tandem mass spectrometry

Cited by 35 publications

References 47 publications

Determination of phenolic and triterpenic compounds in Jatropha gossypiifolia L by Ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS)

Determination of phenolic and triterpenic compounds in Jatropha gossypiifolia L by Ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS)

Antimicrobial, Cyto and Genotoxic Activities of Equisetum hyemale

Suitable simple and fast methods for selective isolation of phospholipids as a tool for their analysis

Contact Info

Product

Resources

About