Study of Inorganic Particles, Fibers, and Asbestos Bodies by Variable Pressure Scanning Electron Microscopy with Annexed Energy Dispersive Spectroscopy and Micro-Raman Spectroscopy in Thin Sections of Lung and Pleural Plaque

Rinaudo, Caterina; Croce, Alessandro; Musa, Maya; Fornero, Elsa; Allegrina, Mario; Trivero, P.; Bellis, Donata; Sferch, Daniela; Toffalorio, Francesca; Veronesi, Giulia; Pelosi, Giuseppe

doi:10.1366/000370210791414380

Cited by 25 publications

(43 citation statements)

References 35 publications

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“…A typical Raman spectrum recorded from the core of the sample is reported in Figure 4. Besides the bands ascribed to fluorapatite (1053,1042,965,606,592, 432 cm −1 ) bands at 660, 413, 295, and 227 cm −1 were also observed, identifying hematite (Chourpa et al 2005, Jubb & Allen 2010, Rinaudo et al 2010. This mineral phase constitutes impurities in the samples and accounts for the iron content detected by the chemical analyses (Table 1).…”

Section: Characterization Of As-rich F-apatitementioning

confidence: 84%

As-RICH APATITE FROM MT. CALVARIO: CHARACTERIZATION BY ΜICRO-RAMAN SPECTROSCOPY

Gianfagna¹,

Mazziotti-Tagliani²,

Croce³

et al. 2014

Can Mineral

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In order to investigate content and localization of arsenic in natural fluorapatite, a micro-Raman spectroscopic study was carried out on As-rich fluorapatite crystals from the volcanic region of Mt. Calvario (Mount Etna, Italy). The crystals, from both the unaltered lavas and the metasomatized rocks, show a pure fluorapatite composition in the core, but an arsenic content of up to 15 wt.% As 2 O 5 in the rim of the crystals from the altered lavas. [AsO 4 ] 3− ⇔ [PO 4 ] 3− substitutions are proposed on the basis of chemical analysis performed with Electron Microprobe (EMP), Scanning Electron Microscopy (SEM-EDS), and Raman spectroscopy. Micro-Raman investigations carried out on the same crystals studied by EMP show a large band, anomalous for fluorapatite, near 860 cm −1 , where chemical microanalyses revealed high contents of As 2 O 5. The Raman broad band near 860 cm −1 is produced by the vibrational modes of [AsO 4 ] 3− tetrahedra. No evidence of arsenate phase crystallization was found, therefore it is proposed that [AsO 4 ] 3− groups substitute for [PO 4 ] 3− groups in the apatite structure. Through μ-Raman spectroscopy, it has been possible to relate the iron content revealed by chemical analysis to magnetite in the fluorapatite from the unaltered lava, and to hematite inclusions in the inner core of the fluorapatite from metasomatized areas.

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Section: Characterization Of As-rich F-apatitementioning

confidence: 84%

As-RICH APATITE FROM MT. CALVARIO: CHARACTERIZATION BY ΜICRO-RAMAN SPECTROSCOPY

Gianfagna¹,

Mazziotti-Tagliani²,

Croce³

et al. 2014

Can Mineral

Self Cite

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“…While revealing only a minimal concentration of metallic iron, the novelty here resides in the detection of a significant and variable percentage of haematite in the bodies. In fact, a recent work38 has already reported the detection of haematite in asbestos bodies by Raman spectroscopy, however explaining the result as a mere artefact and the outcome of irradiation and thermal transformation of asbestos body ferritin. Since such beam-dependent transformation is unlikely in our study, we suppose that the detected haematite is a result of ferrihydrite (from ferritin) transformation occurring during the long residence time in the asbestos coating.…”

Section: Discussionmentioning

confidence: 94%

The interaction of asbestos and iron in lung tissue revealed by synchrotron-based scanning X-ray microscopy

Pascolo

Gianoncelli

Schneider

et al. 2013

Sci Rep

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Asbestos is a potent carcinogen associated with malignant mesothelioma and lung cancer but its carcinogenic mechanisms are still poorly understood. Asbestos toxicity is ascribed to its particular physico-chemical characteristics, and one of them is the presence of and ability to adsorb iron, which may cause an alteration of iron homeostasis in the tissue. This observational study reports a combination of advanced synchrotron-based X-ray imaging and micro-spectroscopic methods that provide correlative morphological and chemical information for shedding light on iron mobilization features during asbestos permanence in lung tissue. The results show that the processes responsible for the unusual distribution of iron at different stages of interaction with the fibres also involve calcium, phosphorus and magnesium. It has been confirmed that the dominant iron form present in asbestos bodies is ferritin, while the concurrent presence of haematite suggests alteration of iron chemistry during asbestos body permanence.

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“…Optical and electron microscopies are most often used to locate and characterize the asbestos fibers [14-17]. In histological examinations, conventional optical microscopy detects the presence of ferruginous bodies but not that of the naked fibres, since most of them are too thin and their detection requires transmission or scanning electron microscopes [15].…”

Section: Introductionmentioning

confidence: 99%

Synchrotron soft X-ray imaging and fluorescence microscopy reveal novel features of asbestos body morphology and composition in human lung tissues

Pascolo¹,

Gianoncelli²,

Kaulich³

et al. 2011

Part Fibre Toxicol

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BackgroundOccupational or environmental exposure to asbestos fibres is associated with pleural and parenchymal lung diseases. A histopathologic hallmark of exposure to asbestos is the presence in lung parenchyma of the so-called asbestos bodies. They are the final product of biomineralization processes resulting in deposition of endogenous iron and organic matter (mainly proteins) around the inhaled asbestos fibres. For shedding light on the formation mechanisms of asbestos bodies it is of fundamental importance to characterize at the same length scales not only their structural morphology and chemical composition but also to correlate them to the possible alterations in the local composition of the surrounding tissues. Here we report the first correlative morphological and chemical characterization of untreated paraffinated histological lung tissue samples with asbestos bodies by means of soft X-ray imaging and X-Ray Fluorescence (XRF) microscopy, which reveals new features in the elemental lateral distribution.ResultsThe X-ray absorption and phase contrast images and the simultaneously monitored XRF maps of tissue samples have revealed the location, distribution and elemental composition of asbestos bodies and associated nanometric structures. The observed specific morphology and differences in the local Si, Fe, O and Mg content provide distinct fingerprints characteristic for the core asbestos fibre and the ferruginous body. The highest Si content is found in the asbestos fibre, while the shell and ferruginous bodies are characterized by strongly increased content of Mg, Fe and O compared to the adjacent tissue. The XRF and SEM-EDX analyses of the extracted asbestos bodies confirmed an enhanced Mg deposition in the organic asbestos coating.ConclusionsThe present report demonstrates the potential of the advanced synchrotron-based X-ray imaging and microspectroscopy techniques for studying the response of the lung tissue to the presence of asbestos fibres. The new results obtained by simultaneous structural and chemical analysis of tissue specimen have provided clear evidence that Mg, in addition to Fe, is also involved in the formation mechanisms of asbestos bodies. This is the first important step to further thorough investigations that will shed light on the physiopathological role of Mg in tissue response to the asbestos toxicity.

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Study of Inorganic Particles, Fibers, and Asbestos Bodies by Variable Pressure Scanning Electron Microscopy with Annexed Energy Dispersive Spectroscopy and Micro-Raman Spectroscopy in Thin Sections of Lung and Pleural Plaque

Cited by 25 publications

References 35 publications

As-RICH APATITE FROM MT. CALVARIO: CHARACTERIZATION BY ΜICRO-RAMAN SPECTROSCOPY

As-RICH APATITE FROM MT. CALVARIO: CHARACTERIZATION BY ΜICRO-RAMAN SPECTROSCOPY

The interaction of asbestos and iron in lung tissue revealed by synchrotron-based scanning X-ray microscopy

Synchrotron soft X-ray imaging and fluorescence microscopy reveal novel features of asbestos body morphology and composition in human lung tissues

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