Background: In most African countries, including Ghana, treatment of the Neisseria gonorrhoeae infection is based on syndromic management owing to lack of laboratory equipment and resources in primary care facilities where most patients first visit. Aim: The aim of this study was to determine the prevalence of Neisseria gonorrhoeae and evaluate its susceptibility pattern to standard antimicrobials used for empirical treatment of the infection in patients that attended Ellolab Diagnostic Centre at Kumasi from November 2014 to July 2017. Methodology: Four hundred and twenty-seven (427) clinical specimens from suspected patients were cultured on chocolate agar. Positive cultures were tested for resistance against twelve Original Research Article