Study of in vivo biocompatibility and dynamics of replacement of rat shin defect with porous granulated bioceramic materials

Чиссов, В И; Свиридова, И. К.; Sergeeva, N. S.; Франк, Г. А.; Кирсанова, В. А.; Achmedova, S. A.; Решетов, И. В.; Filjushin, M. M.; Баринов, С. М.; Фадеева, И. В.; Komlev, V. S.

doi:10.1007/s10517-008-0222-3

Cited by 9 publications

(7 citation statements)

References 10 publications

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“…As defined by the principle of osteoconduction, the graft particles serve as scaffold for the new bone growth in close proximity to surface and engulfed in the pores of biomaterial particles 46 leading to de novo bone formation in the space between the particles or the space created after their slow degradation. 21,[47][48][49] The formation of new bone appears to follow a similar pattern in this study as shown for both groups by micro-CT and histomorphometry, but there is a remarkable upregulated expression of TRAP in the BCP group. Even though both Often characterized as intermediate to slow.…”

Section: Groupsupporting

confidence: 82%

Gene expression, micro‐CT and histomorphometrical analysis of sinus floor augmentation with biphasic calcium phosphate and deproteinized bovine bone mineral: A randomized controlled clinical trial

Arunjaroensuk,

Nampuksa,

Monmaturapoj

et al. 2024

Clin Implant Dent Rel Res

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AimsThe aim of this randomized controlled clinical trial was to compare the gene expression, micro‐CT, histomorphometrical analysis between biphasic calcium phosphate (BCP) of 70/30 ratio and deproteinized bovine bone mineral (DBBM) in sinus augmentation.Materials and MethodsTwenty‐four patients in need for sinus floor augmentation through lateral approach were randomized into BCP 70/30 ratio or DBBM. After at least 6 months of healing, a total of 24 bone specimens were collected from the entire height of the augmented bone at the area of implant placement and underwent micro‐CT, histomorphometric and gene expression analysis. The 12 bone specimens of BCP 70/30 ratio were equally allocated to micro‐CT and histologic analysis (test group, n = 6) and gene expression analysis (test group, n = 6). Similarly, the 12 bone specimens of DBBM were also allocated to micro‐CT and histologic analysis (control group, n = 6) and gene expression analysis (control group, n = 6). The newly formed bone, remaining graft materials and relative change in gene expression of four target genes were assessed.ResultsThe micro‐CT results showed no statistically significant difference in the ratio of bone volume to total volume (BV/TV ratio) for the two groups (BCP 41.51% vs. DBBM 40.97%) and the same was true for residual graft material to total volume (GV/TV ratio, BCP 9.97% vs. DBBM 14.41%). Similarly, no significant difference was shown in the histological analysis in terms of bone formation, (BCP 31.43% vs. DBBM was 30.09%) and residual graft area (DBBM 40.76% vs. BCP 45.06%). With regards to gene expression, the level of ALP was lower in both groups of bone grafted specimens compared with the native bone. On the contrary, the level of OSX, IL‐1B and TRAP was higher in augmented bone of both groups compared with the native bone. However, the relative difference in all gene expressions between BCP and DBBM group was not significant.ConclusionsThe BCP, HA/β‐TCP ratio of 70/30 presented similar histological and micro‐CT outcomes in terms of new bone formation and residual graft particles with DBBM. The gene expression analysis revealed different gene expression patterns between augmented and native bone, but showed no significant difference between the two biomaterials.

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Section: Groupsupporting

confidence: 82%

Gene expression, micro‐CT and histomorphometrical analysis of sinus floor augmentation with biphasic calcium phosphate and deproteinized bovine bone mineral: A randomized controlled clinical trial

Arunjaroensuk,

Nampuksa,

Monmaturapoj

et al. 2024

Clin Implant Dent Rel Res

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“…Reduction of the particle size [4], an increase in porosity [5] and introduction of ionic substitutions, such as magnesium [6], sodium [7], fluorine [8] and/or carbonate [9], so as to mimic the composition and microstructure of biological apatite, have presented approaches to resolving this issue, though with limited success. Namely, even the least sparingly soluble calcium-deficient apatites usually resorb slower than the new bone tissue formation rate [10], whereas HAP with high porosity is weak even to compression and not suitable for load-bearing applications [11]. An alternative approach has comprised admixing more resorbable calcium phosphates, typically tricalcium [12] or dicalcium [13] phosphates, or other calcium compounds, such as sulfates [14] or carbonates [15], to pure HAP.…”

Section: Introductionmentioning

confidence: 99%

Is there a relationship between solubility and resorbability of different calcium phosphate phases in vitro ?

Uskoković

2016

Biochimica et Biophysica Acta (BBA) - General Subjects

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BACKGROUND Does chemistry govern biology or it is the other way around - that is a broad connotation of the question that this study attempted to answer. METHOD Comparison was made between the solubility and osteoclastic resorbability of four fundamentally different monophasic CP powders with monodisperse particle size distributions: alkaline hydroxyapatite (HAP), acidic monetite (DCP), β-calcium pyrophosphate (CPP), and amorphous CP (ACP). RESULTS With the exception of CPP, the difference in solubility between different calcium phosphate (CP) phases became neither mitigated nor reversed, but augmented in the resorptive osteoclastic milieu. Thus, DCP, a phase with the highest solubility, was also resorbed more intensely than any other CP phase, whereas HAP, a phase with the lowest solubility, was resorbed least. CPP becomes retained inside the cells for the longest period of time, indicating hindered digestion of only this particular type of CP. Osteoclastogenesis was mildly hindered in the presence of HAP, ACP and DCP, but not in the presence of CPP. The most viable CP powder with respect to the mitochondrial succinic dehydrogenase activity was the one present in natural biological bone tissues: HAP. CONCLUSION Chemistry in this case does have a direct effect on biology. Biology neither overrides nor reverses the chemical propensities of inorganics with which it interacts, but rather augments and takes a direct advantage of them. SIGNIFICANCE These findings set the fundamental basis for designing the chemical makeup of CP and other biosoluble components of tissue engineering constructs for their most optimal resorption and tissue regeneration response.

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“…HA is widely used due to its excellent biocompatibility and osteoconductivity in dentistry [12,13], but its biodegradability is poor, as such, the graft material remains in defects for a long period of time and ultimately blocks new bone formation [14,15]. The effort to improve the degradation rate of HA through granting porosity faces a limitation since it results in weaker compressive strength unsuitable for load-bearing application [16].…”

Section: Introductionmentioning

confidence: 99%

Effects of BMP-2 Delivery in Calcium Phosphate Bone Graft Materials with Different Compositions on Bone Regeneration

et al. 2016

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This study was undertaken to investigate the effect of loading rhBMP-2 onto biphasic calcium phosphate (BCP) and calcium pyrophosphate (CPP) on bone regeneration, and to examine the efficacies of BCP and CPP as rhBMP-2 carriers. Specimens were divided into the BCP, CPP, BCP/BMP, and CPP/BMP groups; BCP and CPP were in granules and not coated with rhBMP-2. BCP/BMP and CPP/BMP were prepared as discs, which were treated with rhBMP-2 and collagen. Physical and biological features were investigated using in-vitro and in-vivo tests. New bone area percentages (%) in the BCP/BMP and CPP/BMP groups were significantly greater than in the BCP and CPP groups. At weeks 4 and 8 post-implantation, CPP/BMP showed the most new bone growth. Within the limitations of this study, treatment of BCP and CPP with rhBMP-2 significantly enhanced bone regeneration. CPP was found to be a suitable carrier for rhBMP-2.

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Study of in vivo biocompatibility and dynamics of replacement of rat shin defect with porous granulated bioceramic materials

Cited by 9 publications

References 10 publications

Gene expression, micro‐CT and histomorphometrical analysis of sinus floor augmentation with biphasic calcium phosphate and deproteinized bovine bone mineral: A randomized controlled clinical trial

Gene expression, micro‐CT and histomorphometrical analysis of sinus floor augmentation with biphasic calcium phosphate and deproteinized bovine bone mineral: A randomized controlled clinical trial

Is there a relationship between solubility and resorbability of different calcium phosphate phases in vitro ?

Effects of BMP-2 Delivery in Calcium Phosphate Bone Graft Materials with Different Compositions on Bone Regeneration

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