Study of cellulolytic enzyme immobilization on copolymers of N-vinylformamide

Tąta, Agnieszka; Sokołowska, Katarzyna; Świder, Joanna; Konieczna‐Molenda, Anna; Proniewicz, Leonard M.; Witek, E

doi:10.1016/j.saa.2015.04.112

Cited by 20 publications

(10 citation statements)

References 34 publications

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“…The use of a biological approach in the degradation of hydrogen cyanide is a relatively cheaper approach in food processing. Commercial enzymes such as linamarase, xylanase, and cellulase have been successfully demonstrated in the degradation of cyanogenic glucosides in cassava and cassava-based food products to acceptable limits [ 103 , 104 ]. Contrary, Yamashita et al [ 99 ] observed that flaxseeds had a low substrate specificity after experimenting on similar commercial enzymes linamarase from Phaseolus lunatus and β-glucosidase from Prunus dulcis which yielded low activity.…”

Section: Techniques For the Detoxification Of Cyanogenic Glycosidementioning

confidence: 99%

Bioprocessing of Functional Ingredients from Flaxseed

et al. 2018

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Flaxseeds (Linum usitatissimum L.) are oilseeds endowed with nutritional constituents such as lignans, lipids, proteins, fibre, carbohydrates, and micronutrients. Owing to their established high nutritional profile, flaxseeds have gained an established reputation as a dietary source of high value functional ingredients. Through the application of varied bioprocessing techniques, these essential constituents in flaxseeds can be made bioavailable for different applications such as nutraceuticals, cosmetics, and food industry. However, despite their food and health applications, flaxseeds contain high levels of phytotoxic compounds such as linatine, phytic acids, protease inhibitors, and cyanogenic glycosides. Epidemiological studies have shown that the consumption of these compounds can lead to poor bioavailability of essential nutrients and/or health complications. As such, these components must be removed or inactivated to physiologically undetectable limits to render flaxseeds safe for consumption. Herein, critical description of the types, characteristics, and bioprocessing of functional ingredients in flaxseed is presented.

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Section: Techniques For the Detoxification Of Cyanogenic Glycosidementioning

confidence: 99%

Bioprocessing of Functional Ingredients from Flaxseed

et al. 2018

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“…The explanation of this might be found in the reduction in amorphous cellulose regions caused by cellulase preferential attack on them. This preference is due to the larger accessibility presented by β-1,4 glycosidic bonds in these domains (Tąta et al, 2015).…”

Section: Equation 3 Equationmentioning

confidence: 99%

A novel enzymatic approach to nanocrystalline cellulose preparation

Beltramino

Roncero

Vidal

et al. 2018

Carbohydrate Polymers

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In this work, conditions for an enzymatic pretreatment prior to NCC isolation from cotton linter were assessed. Different cellulase doses and reaction times were studied within an experimental design and NCC were obtained. At optimal enzymatic conditions (20U, 2 h), a total yield greater than 80% was achieved and the necessary enzymatic treatment time was reduced 90%. Different intensities of enzymatic treatments led to proportional decreases in fiber length and viscosity and also were inversely proportional to the amount of released oligosaccharides. These differences within fibers lead to quantitative differences in NCC: increase in acid hydrolysis yield, reduction of NCC surface charge and crystallinity increase. Benefits produced by enzymatic treatments did not have influence over other NCC characteristics such as their sulfur content (≈1%), size (≈200 nm), zeta potential (≈-50 mV) or degree of polymerization (≈200). Evidence presented in this work would reduce the use of harsh sulfuric acid generating a cleaner stream of profitable oligosaccharides.

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“…The support can be hydrophilic or hydrophobic; this can be modified, for instance with epoxides, aldehydes or carbodiimides to allow efficient immobilization [7,9,10]. Supports used for enzyme immobilization include vinylformamide based copolymers [11], modified chitosan beads [12], silicate clay minerals [13], carrageenan [14], cellulose, pectin [15], starch [16], zeolites [17], ceramics [18], modified cycloaliphatic epoxide [19] and activated carbon [20].…”

Section: Introductionmentioning

confidence: 99%

Efficient Physisorption of Candida Antarctica Lipase B on Polypropylene Beads and Application for Polyester Synthesis

et al. 2018

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In the present work, Candida antarctica lipase B (CaLB) was adsorbed onto polypropylene beads using different reaction conditions, in order to investigate their influence on the immobilization process and the enzyme activity of the preparations in polymerization reactions. In general, lower salt concentrations were more favorable for the binding of enzyme to the carrier. Polymerisation of dimethyl adipate (DMA) and 1,4-butanediol (BDO) was investigated in thin-film systems at 70 °C and at both atmosphere pressure (1000 mbar) and 70 mbar. Conversion rates and molecular masses of the reaction products were compared with reactions catalyzed by CaLB in its commercially available form, known as Novozym 435 (CaLB immobilized on macroporous acrylic resin). The best results according to molecular weight and monomer conversion after 24 h reaction time were obtained with CaLB immobilized in 0.1 M Na2HPO4\NaH2PO4 buffer at pH 8, producing polyesters with 4 kDa at conversion rates of 96% under low pressure conditions. The stability of this preparation was studied in a simulated continuous polymerization process at 70 °C, 70 mbar for 4 h reaction time. The data of this continuous polymerizations show that the preparation produces lower molecular weights at lower conversion rates, but is comparable to the commercial enzyme concerning stability for 10 cycles. However, after 24 h reaction time, using our optimum preparation, higher molecular weight polyesters (4 kDa versus 3.1 kDa) were obtained when compared to Novozym 435.

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Study of cellulolytic enzyme immobilization on copolymers of N-vinylformamide

Cited by 20 publications

References 34 publications

Bioprocessing of Functional Ingredients from Flaxseed

Bioprocessing of Functional Ingredients from Flaxseed

A novel enzymatic approach to nanocrystalline cellulose preparation

Efficient Physisorption of Candida Antarctica Lipase B on Polypropylene Beads and Application for Polyester Synthesis

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