Study of Antimicrobial Activity of Lime Juice against &lt;i&gt;Vibrio cholerae&lt;/i&gt;

Jayana, Bina Laxmi; Prasai, Tista; Singh, Anjana; Yami, Kayo Devi

doi:10.3126/sw.v8i8.3847

Cited by 18 publications

(15 citation statements)

References 4 publications

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“…Among the tested accessions (Valencia orange, Balady mandarin, Eureka and Rough lemon, Balady lime, Balady sour orange, Duncan grapefruit and Egyptian pummelo), that represent different citrus groups, lime and lemon juices exhibited the highest biological activity. Lemons and limes have been known as an important medicinal plants and the potentiality of their juices as antimicrobial agents was previously confirmed (Tomotake et al, 2006;Jayana et al, 2010;Bansode and Chavan, 2012;Hindi and Chabuck, 2013). In general, antimicrobial activity of Citrus may be referred to their rich content of flavonoids.…”

Section: Antibacterial Activitymentioning

confidence: 88%

Chemical analyses, antibacterial activity and genetic diversity assessment of some Egyptian Citrus spp. cultivars

Youseif¹,

Halwagi²,

Sayed³

et al. 2014

Afr. J. Biotechnol.

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Citrus species are among the most important fruit trees in the world and are considered as a major export product of Egypt. Forty-eight Citrus L. accessions representing six citrus groups (orange, mandarin, lemon, sour orange, grape fruit and pummelo) were collected. Chemical proprieties including pH, total acidity, total soluble solids and ascorbic acid of different fruit juices were determined. Eight accessions representing different citrus groups were screened for their antibacterial activity against five pathogenic bacteria (Escherichia coli, Salmonella typhi, Staphylococcus aureus, Micrococcus spp. and Bacillus pumilus). Lemon and lime accessions exhibited the highest antibacterial activity compared to the standard antibiotics (ampicillin and streptomycin). However, grapefruit and pummelo accessions showed no inhibitory effect. Inter-simple sequence repeats (ISSR) markers were used to study the genetic diversity and phylogenetic relationships among citrus accessions. The highest level of polymorphism (71%) was detected amongst lemon and lime accessions, whereas, the lowest percentage of polymorphism (18%) was identified within the sour orange group. The phylogenetic tree separated the varieties into discrete clusters according to their respective citrus group. Citrus groups were initially divided into two main clusters at 0.18 level of similarity. Lemon, lime, mandarin and sour orange were grouped in the first cluster, while sweet orange, grapefruit and pummelo were nested in the second cluster.

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Section: Antibacterial Activitymentioning

confidence: 88%

Chemical analyses, antibacterial activity and genetic diversity assessment of some Egyptian Citrus spp. cultivars

Youseif¹,

Halwagi²,

Sayed³

et al. 2014

Afr. J. Biotechnol.

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“…The results reported herein show that lime juice has bactericidal activities and can productively remove microorganisms from fomite surfaces. Further studies should focus on identifying the bioactive compounds present in lime juice and their potential for use in chemical and pharmacological applications [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Preliminary Study of the Potential Extracts from Selected Plants to Improve Surface Cleaning

Vong

Chong

Lim

2018

Plants

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Environment hygiene is important for preventing infection and promoting a healthier environment in which to live or work. The goal of this study was to examine the antimicrobial effects of Citrus aurantifolia (key lime) juice and aqueous extracts of Cinnamomum iners (cinnamon) bark and Citrus hystrix (kaffir lime) leaves on the kinetic growth of Pseudomonas aeruginosa and methicillin resistance Staphylococcus aureus (MRSA). Antimicrobial activity was quantitatively evaluated using spectrophotometry and viable cell counts versus bacterial growth time. The fomite surface samples that were used in the second experiment were chosen randomly from the laboratories. They were assessed both before and after intervention using a mixture of commercial disinfectant detergent and lime juice. In the kinetic growth study, the lime juice effectively eliminated P. aeruginosa and MRSA. The cinnamon bark extract was more effective at inhibiting P. aeruginosa than MRSA. The kaffir lime leaf extract demonstrated bacteriostatic activity for the first 60 min, which then weakened after 90 min for both bacteria. The lime juice extract and commercial disinfectant mixture effectively disinfected the fomites. Further studies of the use of key lime juice as a disinfectant in the hospital environment should be conducted, as C. aurantifolia exhibits antibacterial activities against endemic microbes.

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“…Razak et al (2012) menyimpulkan bahwa semakin besar konsentrasi air perasan jeruk nipis yang diberikan, maka daya hambat terhadap bakteri Staphylococcus aureus semakin besar pula [3]. Jayana et al (2010) menguji efek antimikroba air perasan jeruk nipis pada beberapa spesies bakteri yang berbeda dengan metode difusi agar. Zona hambat terbentuk pada bakteri Vibrio cholera, Enterobacter sp, Citrobacter, dan Esherichia coli [4].…”

Section: Pendahuluanunclassified

“…Jayana et al (2010) menguji efek antimikroba air perasan jeruk nipis pada beberapa spesies bakteri yang berbeda dengan metode difusi agar. Zona hambat terbentuk pada bakteri Vibrio cholera, Enterobacter sp, Citrobacter, dan Esherichia coli [4].…”

Section: Pendahuluanunclassified

Formulasi Sediaan Sabun Cuci Tangan Ekstrak Jeruk Nipis (Citrus aurantifolia) dan Efektivitasnya sebagai Antiseptik

Jayani

Kartini

Basirah

2018

MPI

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ABSTRAK:Citrus aurantifolia (jeruk nipis) merupakan bahan alam yang memiliki potensi aktivitas sebagai antiseptik. Penelitian ini bertujuan untuk memformulasi sabun cuci tangan cair dan untuk mengevaluasi aktivitas antiseptiknya. Dibuat tiga formula cabun cuci tangan (Formula I, Formula II, dan Formula III, dengan konsentrasi air perasan jeruk nipis masing-masing 20, 30, dan 40%). Evaluasi meliputi pH dan karakteristik fisik sediaan yang terdiri dari organoleptis, bobot jenis, dan viskositas. Uji efek antiseptik dilakukan dengan menggunakan metode replika. Hasil pengujian organoleptis menunjukkan sabun cair bersifat transparan, homogen, dan berwarna kuning pucat dengan bau khas jeruk nipis. Hasil pengujian viskositas dan pH sediaan belum memenuhi kriteria Standar Nasional Indonesia (SNI). Peningkatan konsentrasi air perasan jeruk nipis membuat penurunan yang signifikan pada viskositas dan pH. Uji efek antiseptik ditunjukkan dengan persentase reduksi bakteri. Persentase reduksi bakteri pada Formula I, II, dan III berturut-turut adalah 61,25; 68,89; dan 89,06%. Terlihat bahwa peningkatan konsentrasi air perasan jeruk nipis dapat mengurangi jumlah koloni bakteri dengan lebih baik. Uji statistika dengan Anova one-way (α = 0,05) yang dilanjutkan dengan LSD menunjukkan bahwa persentase reduksi bakteri pada ketiga formula berbeda secara bermakna dibandingkan dengan formula kontrol (persentase reduksi bakteri = 35,8%). Namun demikian, jika dibandingkan dengan kontrol positif (persentase reduksi bakteri = 71,79%) menunjukkan perbedaan yang tidak bermakna.Kata Kunci: Citrus aurantifolia; sabun cuci tangan; karakteristik; pH; antiseptik (Formula I, Formula II, and Formula III, with a concentration of lime juice 20, 30, and 40%, respectively) Formula I, II, and III were 61.25, 68.89, and 89.06%, respectively. Statistical evaluation using one-way Anova (α = 0.05) followed by LSD showed the percentage of bacterial reduction in three formulas were significantly different compared to the control formula (percentage of bacterial reduction = 35.8%). However, when the result were compared to the positive control (percentage of bacterial reduction = 71.79%), there was no significant difference. ABSTRACT: Citrus aurantifolia (lime) is the natural ingredients having potential activity as an antiseptic. This study aimed to formulate a hand wash liquid soap with active ingredients lime juice and to evaluate its antiseptic activity. Three different formulas

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Study of Antimicrobial Activity of Lime Juice against <i>Vibrio cholerae</i>

Cited by 18 publications

References 4 publications

Chemical analyses, antibacterial activity and genetic diversity assessment of some Egyptian Citrus spp. cultivars

Chemical analyses, antibacterial activity and genetic diversity assessment of some Egyptian Citrus spp. cultivars

Preliminary Study of the Potential Extracts from Selected Plants to Improve Surface Cleaning

Formulasi Sediaan Sabun Cuci Tangan Ekstrak Jeruk Nipis (Citrus aurantifolia) dan Efektivitasnya sebagai Antiseptik

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