Studies related to progesterone-induced hamster sperm acrosome reaction

Suppression of progesterone-enhanced hyperactivation in hamster spermatozoa by estrogen

“…In human spermatozoa, progesterone stimulates calcium influx, tyrosine phosphorylation, chloride efflux, and cAMP increase (Lösel & Wehling 2003, Harper et al 2004, Luconi et al 2004, Baldi et al 2009). It also stimulates ZP penetration and the AR in hamster spermatozoa (Libersky & Boatman 1995b, Llanos & Anabalon 1996. Although spermatozoa do not have a classic intracellular progesterone receptor (PGR), a PGR is present in the cell membrane (Sabeur et al 1996, Baldi et al 1998, 2009, Lösel & Wehling 2003, Luconi et al 2004, Jang & Yi 2005.…”

Acrosome Reaction in the Epididymal Sperm of Chinchilla lanigera. Effect of Progesterone, A23187 and Stimulation of a GABAA‐Like Receptor

“…Our results show, a biphasic behavior of the hormone with a rapid increase in the percentage of reacted sperm between 2.5 and 5 μM with a maximum (71%) with a concentration of 20 μM, and a decrease in the percentage of reacted sperm at higher concentrations. Our results differ from those reported by Ponzio et al () since for the concentration of 20 μM these authors obtained values of AR significantly lower (30%) than the ones obtained in our work, which are consistent with those described by Lanos and Anabalon () for hamster sperm, but are higher than those described for humans (Osman et al, ), and pigs (Melendrez et al, ). On the other hand, our assays with different concentrations of this inducer revealed a deleterious effect on sperm motility that was not observed by Ponzio et al ().…”

Association of Lactate, Intracellular pH, and Intracellular Calcium During Capacitation and Acrosome Reaction: Contribution of Hamster Sperm Dihydrolipoamide Dehydrogenase, the E3 Subunit of Pyruvate Dehydrogenase Complex

“…Treatment with progesterone (physiologic stimulator known to increase intracellular calcium; Blackmore et al, 1990; Llanos and Anabalon, 1996) significantly improved the acrosome reaction ( P < .05) in MT spermatozoa, substantiating the fact that improving the compromised calcium levels in these spermatozoa improves their acrosome reaction (Table 2). These data corroborate well with the increased [Ca 2+ ] i levels seen in the MT spermatozoa (Figure 4C).…”