Studies on the Transmission of a Tigecycline Resistance-Mediating
            <i>tet</i>
            (A) Gene Variant from Enterobacter hormaechei via a Two-Step Recombination Process

Yu, Ran; Chen, Zheng; Li, Danyang; Schwarz, Štefan; Wang, Xinwei; Du, Xiang-Dang

doi:10.1128/spectrum.00496-22

Cited by 6 publications

(3 citation statements)

References 29 publications

(28 reference statements)

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“…Previous studies have identified the tet (A) variant as the reason for tigecycline resistance in Salmonella, Klebsiella pneumoniae , and Enterobacter hormaechei . 24 , 28 , 29 A previous study found that most of the S . Kentucky ST198.2 isolates carried the tet (A) variant and exhibited resistance to tigecycline.…”

Section: Discussionmentioning

confidence: 99%

Persistent Colonization of Ciprofloxacin-Resistant and Extended-Spectrum β-Lactamase (ESBL)-Producing Salmonella enterica Serovar Kentucky ST198 in a Patient with Inflammatory Bowel Disease

Jiang,

Yang,

Wang

et al. 2024

IDR

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Salmonella enterica serovar Kentucky ST198 has emerged as a global threat to humans. In this study, we aimed to characterize the prolonged carriage of ciprofloxacin-resistant and extended-spectrum β-lactamase (ESBL)-producing S. Kentucky ST198 in a single patient with inflammatory bowel disease (IBD). Methods: Three S. Kentucky strains were collected from a single patient with IBD on 11th January, 23rd January, and 8th February, 2022, respectively. Antimicrobial susceptibility testing, whole-genome sequencing, and phylogenetic analysis with 38 previously described Chinese S. Kentucky ST198 strains from patients and food were performed. Results: All three S. Kentucky isolates belonged to ST198. They carried identical 16 resistance genes, such as bla CTX-M-55 , tet(A), and qnrS1, and had identical mutations within gyrA (S83F and D87N) and parC (S80I). Therefore, they exhibited identical multidrugresistant profiles, including the clinically important antibiotics cephalosporins (ceftazidime and cefepime), fluoroquinolones (ciprofloxacin and levofloxacin), and third-generation tetracycline (tigecycline). Our three S. Kentucky strains were classified into the subclade ST198.2-2, and were genetically identical (2-6 SNPs) to each other. They exhibited a close genetic similarity (15-20 SNPs) to the isolate NT-h3189 from a patient and AH19MCS1 from chicken meat in China, indicating a possible epidemiological link between these S. Kentucky ST198 isolates from the patients and chicken meat. Conclusion: Long-term colonization of ciprofloxacin-resistant and ESBL-producing S. Kentucky ST198 in a single patient is a matter of concern. Due to the potential transfer of S. Kentucky ST198 from food sources to humans, ongoing surveillance of this particular clone in animals, animal-derived food products, and humans should be strengthened.

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Section: Discussionmentioning

confidence: 99%

Persistent Colonization of Ciprofloxacin-Resistant and Extended-Spectrum β-Lactamase (ESBL)-Producing Salmonella enterica Serovar Kentucky ST198 in a Patient with Inflammatory Bowel Disease

Jiang,

Yang,

Wang

et al. 2024

IDR

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“…In addition, except for 35 tigecycline resistance strains carrying tet (X4) or tet (X6), the remaining 66 strains carrying tetracycline resistance genes tet (A), tet (J) and tet (M) were also resistant to tigecycline and 80.3% of these strains were Proteus . It has been found that K. pneumoniae and E. hormaechei carrying the tet (A) variant are resistant to tigecycline due to double frameshift mutation of the tet (A) gene ( 41 , 42 ). And the tet (M) variant can make Streptococcus suis resistant to tigecycline ( 43 ).…”

Section: Discussionmentioning

confidence: 99%

Widespread Dissemination of Plasmid-Mediated Tigecycline Resistance Gene tet (X4) in Enterobacterales of Porcine Origin

et al. 2022

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Tigecycline is considered to be the last resort of defense against diseases caused by broad-spectrum resistant Gram-negative bacteria. In this study, we systematically analyzed the prevalence and genetic environments of the resistance gene tet (X4) in a pig slaughterhouse and farms and the evolutionary relationship of 533 tet (X4)-positive Escherichia coli strains, including 509 tet (X4)-positive E. coli strains selected from the 27,802 assembled genomes of E. coli from the NCBI between 2002 and 2022.

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“…Also, tet (X15) conferred resistance to tetracyclines and glycylcyclines ( Li R. et al., 2021 ). New variants of the tet gene are still emerging, with twenty-seven variants isolated in 2021 ( Yu R. et al., 2022 ). These variants were named from tet (X18) to tet (X44) and were obtained from R. anatipestifer ( Umar et al., 2021 ).…”

Section: Transfer Of Tet Genesmentioning

confidence: 99%

Molecular mechanisms of tigecycline-resistance among Enterobacterales

Korczak,

Majewski,

Iwaniuk

et al. 2024

Front. Cell. Infect. Microbiol.

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The global emergence of antimicrobial resistance to multiple antibiotics has recently become a significant concern. Gram-negative bacteria, known for their ability to acquire mobile genetic elements such as plasmids, represent one of the most hazardous microorganisms. This phenomenon poses a serious threat to public health. Notably, the significance of tigecycline, a member of the antibiotic group glycylcyclines and derivative of tetracyclines has increased. Tigecycline is one of the last-resort antimicrobial drugs used to treat complicated infections caused by multidrug-resistant (MDR) bacteria, extensively drug-resistant (XDR) bacteria or even pan-drug-resistant (PDR) bacteria. The primary mechanisms of tigecycline resistance include efflux pumps’ overexpression, tet genes and outer membrane porins. Efflux pumps are crucial in conferring multi-drug resistance by expelling antibiotics (such as tigecycline by direct expelling) and decreasing their concentration to sub-toxic levels. This review discusses the problem of tigecycline resistance, and provides important information for understanding the existing molecular mechanisms of tigecycline resistance in Enterobacterales. The emergence and spread of pathogens resistant to last-resort therapeutic options stands as a major global healthcare concern, especially when microorganisms are already resistant to carbapenems and/or colistin.

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Studies on the Transmission of a Tigecycline Resistance-Mediating tet (A) Gene Variant from Enterobacter hormaechei via a Two-Step Recombination Process

Cited by 6 publications

References 29 publications

Persistent Colonization of Ciprofloxacin-Resistant and Extended-Spectrum β-Lactamase (ESBL)-Producing Salmonella enterica Serovar Kentucky ST198 in a Patient with Inflammatory Bowel Disease

Persistent Colonization of Ciprofloxacin-Resistant and Extended-Spectrum β-Lactamase (ESBL)-Producing Salmonella enterica Serovar Kentucky ST198 in a Patient with Inflammatory Bowel Disease

Widespread Dissemination of Plasmid-Mediated Tigecycline Resistance Gene tet (X4) in Enterobacterales of Porcine Origin

Molecular mechanisms of tigecycline-resistance among Enterobacterales

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