Studies on the proteins of mass-cultivated, blue-green alga (Spirulina platensis)

Devi, M. Anusuya; Subbulakshmi, G.; Devi, Kiran; Venkataraman, L. V.

doi:10.1021/jf00105a022

Cited by 59 publications

(40 citation statements)

References 2 publications

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“…The overall yield of total nitrogen and hence the yield of total protein in the protein isolate was 60.7%. This value was higher than the yields reported in the literature for proteins extracted using alkali-acid method from green algae (Gerde et al, 2013;Ursu et al, 2014) but lower than the 80% yield reported for S. platensis protein isolates (Devi et al, 1981). The higher yield reported in the latter case was a result of the use of hexane defatted biomass as the starting material and the repeated (three times) aqueous extraction and dialysis steps.…”

Section: Protein Solubility Curvecontrasting

confidence: 59%

“…Second, it has a very high protein content (Cohen, 1997) and hence the impact of protein isolation on the biochemical composition of the residual biomass would be very striking in this species compared to those with a lower protein content. Although some reports on extraction of proteins from S. platensis may be found in the literature, major knowledge gaps on process optimization, component fractionation, and product characterization remain (Devi et al, 1981;Chronakis et al, 2000;Safi et al, 2013b). The current work aimed at filling these gaps in order to understand the fate of various cell components as a result of the fractionation process and identify the bottlenecks in the process.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Protein Extraction from Spirulina platensis to Generate a Potential Co-Product and a Biofuel Feedstock with Reduced Nitrogen Content

et al. 2015

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The current work reports protein extraction from Spirulina platensis cyanobacterial biomass in order to simultaneously generate a potential co-product and a biofuel feedstock with reduced nitrogen content. S. platensis cells were subjected to cell disruption by high-pressure homogenization and subsequent protein isolation by solubilization at alkaline pH followed by precipitation at acidic pH. Response surface methodology was used to optimize the process parameters -pH, extraction (solubilization/precipitation) time and biomass concentration for obtaining maximum protein yield. The optimized process conditions were found to be pH 11.38, solubilization time of 35 min and biomass concentration of 3.6% (w/w) solids for the solubilization step, and pH 4.01 and precipitation time of 60 min for the precipitation step. At the optimized conditions, a high protein yield of 60.7% (w/w) was obtained. The protein isolate (co-product) had a higher protein content [80.6% (w/w)], lower ash [1.9% (w/w)] and mineral content and was enriched in essential amino acids, the nutritious γ-linolenic acid and other high-value unsaturated fatty acids compared to the original biomass. The residual biomass obtained after protein extraction had lower nitrogen content and higher total non-protein content than the original biomass. The loss of about 50% of the total lipids from this fraction did not impact its composition significantly owing to the low lipid content of S. platensis (8.03%).

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Section: Protein Solubility Curvecontrasting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Optimization of Protein Extraction from Spirulina platensis to Generate a Potential Co-Product and a Biofuel Feedstock with Reduced Nitrogen Content

et al. 2015

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“…4, right panel). These values were comparable to the values (50-55%) that had been determined with another A. platensis strain [24].…”

Section: Effect Of Boron Depletion On the Biomass And The Protein Chsupporting

confidence: 89%

Growth of the Edible Microalga Arthrospira Platensis in Relation to Boron Supply

Tadama¹,

Shiraishi²

2017

GEOMATE

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Arthrospira (Spirulina) platensis is an edible cyanobacterium that has been consumed worldwide as a nutrient source under the name spirulina. When preparing synthetic media for this microalga, boron is usually added to them. However, whereas boron is necessary for the N 2 -fixation-dependent growth of heterocystous cyanobacteria, boron requirement by A. platensis, which is non-heterocystous, has not yet been carefully examined. To examine the effect of boron on A. platensis, we prepared a boron-depleted medium in which borate concentration was below a detection limit (0.2 µM), as determined by the spectrophotometric quantitation with H-resorcinol. Using this boron-depleted medium, A. platensis NIES-39 was analyzed for changes in growth, dry biomass weight and the protein, chlorophyll a and C-phycocyanin contents. Experimental results showed that removal of boron from the growth medium had no detectable effect on them. A control experiment with a heterocystous cyanobacterium Anabaena sp. UTEX 2576 showed that the growth of this cyanobacterium was suppressed in a boron-depleted medium. These experiments demonstrated that boron was not required for the growth of A. platensis. Our results indicate that boron supply is not necessary for the propagation of A. platensis even in the regions where boron deficiency in the soil deteriorates the growth of common crops. Use of boron-free media would also be helpful to improve the reproducibility of sensitive physiological experiments such as metabolome analysis, because precipitation of the insoluble salt manganese borate, which may reduce the concentration of Mn 2+ in the medium, does not occur in boron-free media.

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“…Reported IVPD for Chlorella are 55-66 % (Tamiya 1962), 27-93 % (Hedenskog et al 1969), 44-57 % (Janczyk et al 2005), 70-97 % (Morris et al 2008), and 61-79 % (Mišurcova et al 2010). IVPD for Spirulina has been reported to be 84 % (Lipinsky and Litchfield 1974)and 70-85 % (Devi et al 1981), and work with other species (Nostoc, Scenedesmus,a n dMicrocystis)h a v es h o w nI V P D values of 29-93 % (Hedenskog et al 1969;de la Fuente et al 1977;Horietal.1990). This large variability may be related to species differences but more likely due to differences in methods used such as differences in enzyme mixtures, sample processing, assay conditions, and the extent of algal cell wall disruption.…”

Section: −1mentioning

confidence: 99%

Chemical composition and nutritional properties of freshwater and marine microalgal biomass cultured in photobioreactors

2014

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Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/view/object/?id=b8564d17-860a-4eb5-80c6-fd741c419b07 http://nparc.cisti-icist.nrc-cnrc.gc.ca/fra/voir/objet/?id=b8564d17-860a-4eb5-80c6-fd741c419b07 Abstract Proximate, amino acid and elemental composition, total phenolic content (TPC), and in vitro protein digestibility (IVPD) of microalgal biomass were determined. Microalgae contained low to moderate ash (5-17 %), moderate to high carbohydrate (18-46 %), crude protein (18-46 %), high crude lipid (12-48 %), and energy (19-27 MJ kg −1 ). Characteristic of microalgae, non-essential amino acids (AAs), aspartic and glutamic acids, were predominant (20-30 % of protein; 8-12 % of dry weight). Microalgae had favorable essential AA profiles with high essential amino acid (EAA) indices (0.9-1.2). Expressed as gE A A1 0 0gp r o t e i n −1 , Porphyridium aerugineum was rich in leucine (11.9), lysine (8.0), arginine (8.6), and tryptophan (3.3); Nannochloropsis granulata (A) in leucine (11.0), lysine (8.5), and tryptophan (2.8); Tetraselmis chuii and Botryococcus braunii in arginine (9.4 and 20.5, respectively); and Phaeodactylum tricornutum in lysine (6.4) and tryptophan (2.6). Mineral compositions (%) were calcium (0.1-3.0), magnesium (0.3-0.7), phosphorous (0.7-1.5), potassium (0.7-2.4), sodium (0.8-2.7), and sulfur (0.4-1.4), and trace element compositions (mg kg −1 )we reco pp er(1 8 -102), iron (1,395-11,101), manganese (45-454), selenium (0-0.5), and zinc (28-64). Microalgae contained low TPC (6-13 mg gallic acid equivalents (GAE) g −1 DW), except T. chuii (20 mg GAE g −1 DW). IVPD was high (>90 %) for B. braunii (A), P. aerugineum, and lipid-extracted N. granulata (B); mid-range (80-89 %) for P. tricornutum, N. granulata (A), B. braunii (B), Neochloris oleoabundans, T. chuii, and whole N. granulata (B); and lower (<80 %) for Acutodesmus dimorphus.M i c r o a l g a ls p e c i e sP. tricornutum, B. braunii, N. granulata,a n dT. chuii had high protein (40-52 %), IVPD (82-97 %), and digestible protein (35-50 %), comparable to plant proteins used in animal feeds and aquaculture.

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Studies on the proteins of mass-cultivated, blue-green alga (Spirulina platensis)

Cited by 59 publications

References 2 publications

Optimization of Protein Extraction from Spirulina platensis to Generate a Potential Co-Product and a Biofuel Feedstock with Reduced Nitrogen Content

Optimization of Protein Extraction from Spirulina platensis to Generate a Potential Co-Product and a Biofuel Feedstock with Reduced Nitrogen Content

Growth of the Edible Microalga Arthrospira Platensis in Relation to Boron Supply

Chemical composition and nutritional properties of freshwater and marine microalgal biomass cultured in photobioreactors

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