Studies on the metabolism of pyruvic acid in normal and vitamin B1-deficient states

Lu, G. D.

doi:10.1042/bj0330249

Cited by 189 publications

(19 citation statements)

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“…Markees (1951b) has shown that boiling the filtrate for five minutes destroys the aceto-acetic acid (the main competitor of pyruvic acid) without loss of pyruvic acid. Normal Values.-The normal values for blood pyruvic acid in healthy persons obtained in this study (0.53-1.08 mg. %) are in conformity with other reports irrespective of the methods used (Bueding, Wortis, and Stern, 1942;Friedemann and Haugen, 1943;Lu, 1939;Markees, Kaser, and Lanz, 1950).…”

Section: Discussionsupporting

confidence: 92%

“…Since newer methods for its estimation have been introduced by Lu (1939) and by Friedemann and Haugen (1943), several authors have studied the role of pyruvic acid in heart diseases (Altschule and Rosenfeld, 1947;Barenghi, Scarlotti, and Scafidi, 1941;Lasch, 1952;Torre, Teece, and Baldantoni, 1950;Yanof, 1942), and a survey of the changes in pyruvic acid levels in other diseases and in relation to therapy has been published by Markees (1951a).…”

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confidence: 99%

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A Study of the Blood Pyruvic Acid Level in Patients with Congestive Heart Failure

Kleeberg¹,

Gitelson²

1954

Journal of Clinical Pathology

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Section: Discussionsupporting

confidence: 92%

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confidence: 99%

A Study of the Blood Pyruvic Acid Level in Patients with Congestive Heart Failure

Kleeberg¹,

Gitelson²

1954

Journal of Clinical Pathology

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“…Pyruvate in the blood was estimated by a modification, partly suggested by Dr. E. S. Guz' man Barron, of the method of Lu (8). Lactate and pyruvate in the blood were stabilized with fluoride and iodoacetic acid.…”

Section: Methodsmentioning

confidence: 99%

Influence of Methyl Testosterone on Muscular Work and Creatine Metabolism in Normal Young Men1

Samuels

Henschel

Keys

1942

The Journal of Clinical Endocrinology & Metabolism

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“…The d~ uptake was measured, as usual, in Warburg-Barcroft manometers and vessels. Pyruvate was determined colorimetrically by a modification of the method of Lu (14). In experiments in which the pyruvate content of Arbacia eggs was determined, the eggs were centrifuged and 4 volumes of 20 per cent trichloroacetic acid were added.…”

Section: Methodsmentioning

confidence: 99%

The Pyruvate Metabolism of Sea Urchin Eggs During the Process of Cell Division

Goldiger

Barrón

1946

Journal of General Physiology

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Since the classical discovery of Warburg (1) that fertilization of sea urchin eggs produces a sudden increase in the oxygen uptake, numerous investigators have attempted to correlate this increase to chemical changes. Perlzweig and Barron (2) found that the eggs of Arbacia punctulata contain carbohydrates and produce lactic acid. However, no increase in lactic acid formation was detected on fertilization. In 1933, RunnstrSm (3) found that 'during fertilization there is an increase in the formation of acid, the nature of which is still unknown. In 1935, ~3rstrtim (4) reported an increase in ammonia formation, which he attributed to the degradation of purines (5). This ammonia formfftion, in the opinion of Hutchens et al. (6) may be due to protein oxidation; i.e., oxidative deamination. An increased utilization of glycogen was demonstrated by 0rstrSm and Lindberg (7) and by Lindberg (8). A change in the solubility in ether and alcohol of lecithin and cephalin has also been noted: before fertilization all the cephalin is soluble in ether while after fertilization it becomes insoluble (9). Fertilization and membrane formation are essentially anaerobic processes since they can be obtained in the absence of oxygen while nuclear changes and cell division are essentially aerobic processes since they do not occur in the absence of oxygen (10). After fertilization there are an increased permeability of the cell membrane, and a channeling of oxidations via the cytochrome pathway, as shown by the inhibition of respiration of fertilized eggs by HCN and CO in contrast with the insensitivity of the unfertilized eggs to these agents (11). The increased oxygen consumption, added to an increased utilization of glycogen with the formation of an acid is an indication that during the fertilization process there is an increase of the oxidative phase of carbohydrate metabolism. Since the metabolism of carbohydrate in Arbacia eggs under anaerobic conditions ends with the formation of lactic acid, it is reasonable to conclude that the lactic acid comes from the reduction of pyruvic acid. (Arbacia eggs contain from 250 to 500 micrograms of diphosphopyridine nucleotide, the coenzyme for glycolysis, according to Jandorf and Krahl (12).) Hence, the oxidative phase must start either with the oxidation of pyruvic to acetic acid or with its carboxylation to oxaloacetic * An abstract of this work appeared in

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Studies on the metabolism of pyruvic acid in normal and vitamin B1-deficient states

Abstract: Criticisms of some of the previous methods are to be found in references marked with an asterisk.

Cited by 189 publications

References 5 publications

A Study of the Blood Pyruvic Acid Level in Patients with Congestive Heart Failure

A Study of the Blood Pyruvic Acid Level in Patients with Congestive Heart Failure

Influence of Methyl Testosterone on Muscular Work and Creatine Metabolism in Normal Young Men1

The Pyruvate Metabolism of Sea Urchin Eggs During the Process of Cell Division

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