Studies on the metabolism of pyruvic acid in normal and vitamin B1-deficient states

Lu, G. D.

doi:10.1042/bj0330774

Cited by 53 publications

(7 citation statements)

References 8 publications

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“…Details of the experiments are set out in Table 1, and a summary of the results based on the statistical analysis is given in Table 2. The concentration of pyruvate in the blood of normal rabhits was found to agree closely with that reported previously (Lu, 1939), but the normal value for adult sheep was found to he much lower than that observed hy Austin (1947). However, this difference might well he attributed to the different experimental conditions, as it is clear that a state of activity affects the pyruvate concentration in the hlood: to obtain reasonably comparable results it is essential that the animal be maintained in a thoroughly placid condition (Jarrett, Potter and Filsell, 1952).…”

Section: Resultssupporting

confidence: 89%

The Effect of Glucose on Blood Pyruvate in Sheep

Jarrett

1954

Aust J Exp Biol Med

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A correlation exists between the concentration in the blood of total reducing sugar and of pyruvate, an important intermediary in carbohydrate metabolism. Flock, BoUman and Mann (1938) reported that the levels of pyruvate in the blood of dogs were approximately doubled after the injection of a solution of glucose, but no rise occurred after injection of physiological saline. Bueding, Stein and Wortis (1941) observed a temporary rise in blood pyruvate after the administration of glucose to humans and dogs; in a subsequent paper, Bueding, " Wortis and Fein (1942) showed that the ingestion of glucose by human diabetics brought about little increase in blood pyruvate, but if insulin was administered a significant rise occurred under these conditions. Horwitt, Hills and Kreisler (1949) observed a sharp rise in blood pyruvate after the ingestion of glucose by normal human subjects, and that the return to normal was parallel to the blood sugar curve. When glucose is injected intravenously the blood pyruvate remains elevated for approximately 2 hours (Amatuzio et al., 1952).In view of the nature of the intermediary metabolism of the adult ruminant (Marston, 1939;McCandless and Dye, 1950;Jarrett and Potter, 1953), and of the apparent similarity in some respects of a young lamb and a non-ruminant (Jarrett and Potter, 1952), the effect of an injection of glucose on the blood pyruvate level in the young lamb and the adult sheep was compared. METHODS.Five adult Merino ewes that had been sustained on an adequate diet of a wheaten hay and lucerne chaff mixture were fasted overnight prior to each experiment. A small-bore polyethylene tube was inserted for several inches into the jugular vein about an hour preceding the experimental period and filled with a dilute solution of heparin in physiological saline to reduce the possibility of blood clots forming in the tube. Blood samples were obtained with a minimum of handling by attaching a syringe to the end of the tube. This procedure allowed the animal to remain placid throughout the manipulations, a condition which is essential for sueh experiments as these, because activity influences the concentration of pyruvate in the blood.An initial blood sample was taken, and then 100 ml. of glucose solution, containing 1 gm. glucose/kg, body weight were injected slowly over a period of five minutes. Blood samples were taken at intervals of 30, 60 and 90 minutes after the injection, and the concentration of pyruvate determined by the method of Friedemann and Haugen (1943

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Section: Resultssupporting

confidence: 89%

The Effect of Glucose on Blood Pyruvate in Sheep

Jarrett

1954

Aust J Exp Biol Med

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“…For instance, vitamin B 1 deficiency in humans increases blood lactate and pyruvate levels, a finding consistent with an increase in glycolysis and a decrease in glucose oxidation. 367 In addition, many vitamin deficiencies observed in humans can be mimicked in animal models through appropriate dietary manipulations, and the impact on cardiac metabolism can be assessed by standard techniques. For example, vitamin D deficiency results in increased glycolysis and decreased mitochondrial oxidative metabolism in the rat heart.…”

Section: Nongenetic Models For Cardiac Metabolismmentioning

confidence: 99%

Assessing Cardiac Metabolism

Taegtmeyer¹,

Young²,

Lopaschuk³

et al. 2016

Circulation Research

233

147

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In a complex system of interrelated reactions, the heart converts chemical energy to mechanical energy. Energy transfer is achieved through coordinated activation of enzymes, ion channels, and contractile elements, as well as structural and membrane proteins. The heart’s needs for energy are difficult to overestimate. At a time when the cardiovascular research community is discovering a plethora of new molecular methods to assess cardiac metabolism, the methods remain scattered in the literature. The present statement on “Assessing Cardiac Metabolism” seeks to provide a collective and curated resource on methods and models used to investigate established and emerging aspects of cardiac metabolism. Some of those methods are refinements of classic biochemical tools, whereas most others are recent additions from the powerful tools of molecular biology. The aim of this statement is to be useful to many and to do justice to a dynamic field of great complexity.

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“…Bradycardia, as observed in the thiamine deficient rat, has also been attri buted to the accumulation of pyruvate and lactate in the blood and within the myocardium (47)(48)(49). In the present study, perfusionn of rat hearts from both normal and 4-week thiamine deficient animals with neutralized perfusion medium containing pyruvate and lactate levels similar to those reported for severely thiamine deficient rats resulted in only a slight alteration in heart rate that was not of the same magnitude as seen in the deficient animal.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Thiamine Deprivation, and Oxythiamine- And Pyrithiamine-Treatment on Cardiac Function and Metabolism in the Rat

1974

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Studies on the metabolism of pyruvic acid in normal and vitamin B1-deficient states

Cited by 53 publications

References 8 publications

The Effect of Glucose on Blood Pyruvate in Sheep

The Effect of Glucose on Blood Pyruvate in Sheep

Assessing Cardiac Metabolism

The Effects of Thiamine Deprivation, and Oxythiamine- And Pyrithiamine-Treatment on Cardiac Function and Metabolism in the Rat

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